GENDER AND AGE ASPECTS OF BIOENERGETICS PROCESSES IN EXPERIMENTAL PASSIVE TOBACCO SMOKING AND MONOSODIUM GLUTAMATE ADMINISTRATION
DOI:
https://doi.org/10.11603/ijmmr.2413-6077.2018.2.9810Keywords:
passive tobacco smoking, monosodium glutamate, cytochrome oxidase, succinate dehydrogenaseAbstract
Background. Active smoking and exposure to passive smoke are responsible for more than 5 million deaths each year. At the same time, a characteristic feature of present food technologies is the use of food additives that are not always safe for human health, such as monosodium glutamate (MSG).
Objective. The aim of the research was to determine the changes in mitochondrial enzymes activity in rats in case of passive tobacco smoke combined with prolonged administration of MSG in the sex and age aspects.
Methods. The evaluation of bioenergetics processes in the mitochondria of circulating neutrophils was carried out using succinate dehydrogenase (SDG) and cytochrome oxidase (CO) activity.
Results. Passive tobacco smoke combined with the MSG administration in mature male-rats is accompanied by a significant inhibition of bioenergetics processes, as evidenced by a decrease in succinate dehydrogenase activity by 47.1% (p<0.001) compared to the intact animals, which is by 27.9% (p<0.001) below this index in case of the isolated effect of tobacco smoke and reduction of cytochrome oxidase activity by 27.5% (p<0.001) compared to the control group.
Conclusions. Thus, the findings suggest that low dose intake of monosodium glutamate enhances the ability of tobacco smoke to disrupt the cell's bioenergetics processes by affecting the respiratory chain function and generation of ATP. Therefore, it is advisable to investigate the established toxic doses of E621, as well as to study the molecular mechanisms of the ‘safe’ (allowed) doses of MSG effect on a living organism.
References
Reitsma MB. Smoking prevalence and attributable disease burden in 195 countries and territories, 1990-2015: a systematic analysis from the Global Burden of Disease Study 2015. The Lancet, 2017;389(10082),1885-1906.
Xu X, Bishop EE, Kennedy SM, Simpson SA, Pechacek TF. Annual Healthcare Spending Attributable to Cigarette Smoking: An Update. American Journal of Preventive Medicine; 2015;48(3): 326-33.
Solomenchuk TM, Badzai AO, Protsko VV. Metabolic disorders in women with unstable angina, depending on the smoking habit. BukovinianMedical Bulletin; 2017;21,2(1):85-88 [In Ukrainian].
Peirson L, Muhammad UA, Meghan K, Parminder R, Sherifali D. Interventions for prevention and treatment of tobacco smoking in school-aged children and adolescents: A systematic review and meta-analysis. Preventive Medicine; 2016;85:20-31.
Husarova V, Ostatnikova D. Monosodium Glutamate Toxic Effects and Their Implications for Human Intake: A Review. Journal of Medical Internet Research; 2013;2013:1-12.
Likhatsky PG, Fira LS, Gonsky YaI. Dynamics of changes of markers of bioenergetic processes and cytolysis in rats after damage by sodium nitrite against the background of tobacco intoxication. Bulletin of Biology and Medicine Problems; 2017; 2(136):147-152 [In Ukrainian].
Lizurchik LV, Shade EV. Effect of tobacco smoke on the content of toxic elements in the rat. Bull. OGU; 2014;6(167):71-74 [In Russian].
Falaleeva TM, Samonina GE, Beregovaya TV, Dzyubenko NV, Andreev LA. The effect of glyprolines on the structural and functional state of the gastric mucosa and body weight of rats under conditions of prolonged administration of sodium glutamate. Physics of the Living; 2010;18,1:154-159 [In Russian].
Bilyk A, Negelya A, Garmanchuk L. Activity of cytochrome oxidase and succinate dehydrogenase in primary culture of transfected carcinoma of Lewis lungs at different stages of tumor growth. Bulletin of Taras Shevchenko National University of Kyiv; 2016;2(21):81-85 [In Ukrainian].
Voloshchuk ON, Marchenko MM. Enzymatic activity of components of the system of energy supply of mitochondria of blood leukocytes in the growth dynamics of Guerin's carcinoma. Siberian Cancer Journal; 2013;6(60):36-39 [In Russian].
Vasilenko OV, Bodnar OI, Vinyarskaya GB, Sinyuk YuV., Grubinko VV. Energy and nitrogen metabolism of uchlorella vulgaris beij(chlorophyta) under the influence of sodium selenite. Algology; 2014;24(3):297-301 [In Russian].
Yanbaeva DG, Dentener MA, Creutzberg EC, Wesseling G, Wouters EF. Systemic effects of smoking. Chest; 2007;131(5):1557-66.
Heijink IH, Pouwels SD, Leijendekker C, de Bruin HG, Zijlstra GJ, van der Vaart H, ten Hacken NH, van Oosterhout AJ, Nawijn MC, van der Toorn M. Cigarette Smoke–Induced Damage-Associated Molecular Pattern Release from Necrotic Neutrophils Triggers Proinflammatory Mediator Release. American Journal of Respiratory Cell and Molecular Biology. 2015 May;52(5):554-62.
Lykhatskyi PH., Fira LS., Fedorovich UM. Proteins oxidative modification and mitochondrial enzymes activity in rats of different ages under affection by sodium nitrites and tobacco smoke. Ukrainian Biopharmaceutical Journal; 2017;3(50): 38-46.
Sharma A. Monosodium glutamate-induced oxidative kidney damage and possible mechanisms: a mini-review. Journal of Biomedical Science; 2015; 22:93.
Ward MW, Rego AC, Frenguelli BG, Nicholls DG. Mitochondrial Membrane Potential and Glutamate Excitotoxicity in Cultured Cerebellar Granule Cells J Neurosci. 2000;20(19):7208-19.
Wu S, Zhou F, Zhang Z, Xing D. Mitochondrial oxidative stress causes mitochondrial fragmentation via differential modulation of mitochondrial fission-fusion proteins. FEBS J; 2011;278:941-954.
Kumari S, Mehta SL, Li PA. Glutamate Induces Mitochondrial Dynamic Imbalance and Autophagy Activation: Preventive Effects of Selenium. PLoS ONE; 2012;7(6):e39382.
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