ACETAMINOPHEN EFFECT ON FREE RADICAL OXIDATION INDICES IN RATS WITH TYPE 2 DIABETES MELLITUS
Keywords:acetaminophen, malondialdehyde, diene and triene conjugates, Schiff bases, diabetes mellitus.
Background. Acetaminophen is a drug used to relieve pain syndrome. It is used both independently and in composition of combined drugs. Type 2 diabetes is an age-related disease that is associated with a violation of insulin synthesis by pancreas.
Objective. The aim of the research was to study the effect of acetaminophen on major free radical oxidation indices of rats with type 2 diabetes mellitus in time dynamics.
Methods. We conducted two series of experiments. The first series comprised rats with type 2 diabetes mellitus and acute acetaminophen toxic lesions. The second series involved rats with type 2 diabetes mellitus and acetaminophen administration at a dose of 55 mg/kg for the period of 7 days.
Results. Administration of acetaminophen for rats with type 2 diabetes mellitus caused the increase in the content of malondialdehyde, diene and triene conjugates and Schiff bases in blood plasma and malondialdehyde, diene and triene conjugates in liver homogenate. The maximum increase in these indices was observed on the first day of the experiment. Gradually these indices decreased on the 3rd, 5th and 7th days of the experiment.Conclusions. Free radical oxidation increased in both series of the experiment. This process developed in rats with type 2 diabetes mellitus and acute acetaminophen toxic lesions more intensively, than in rats with type 2 diabetes mellitus and administration of acetaminophen at the highest therapeutic dose during 7 days.
Stepanov YuM, Filippova AYu, Kononov IN. Medicinal lesions of liver: pathogenesis, classification, diagnosis, treatment. Pharmacist. 2006;5.
Ushkalova YeA. Medicinal lesions of liver. Gastroenterology. 2003;10(73):72–75.
Buyeverov AO. Fundamental understanding of medicinal lesions of liver. Clinical prospects of gastroenterology, hepatology. 2002;4:7–11.
Minushkin ON. Some hepatoprotectors in the treatment of hepatic diseases. Therapist. 2002;6:55–58.
Sheen CL, Dillon JF, Bateman DN, et al. Paracetamol toxicity: epidemiology, prevention and costs to the health-care system. Q J Med. 2002:95:9:609–619.
Volchegorskiy IA, Nalimov AG, Yarovinskiy BG, Livshits RI. Comparison of different approaches to the determination of lipid peroxidation in heptane-isopropanol extracts of blood.
Questions of medical chemistry. 1989;1:127.
Islam S, Choi H. Nongenetic Model of Type 2 Diabetes: A Comparative Study. Pharmacology. 2007;79:243–249.
Islam S, Loots DT. Experimental rodent model soft type 2 diabetes: a review. Methods Find Exp Clin Pharmacol. 2009;31(4);249–261.
Stalnaya ID, Garishvili TG. The method for determining malonic dialdehyde by means of thiobarbituric acid. Moscow: Medicine: 1977. p. 66–68.
Kozhemyakin YuM, Khromov OS, Filonenko MA. The Guideline Principles for the Care and Use of Laboratory Animals. Kyiv: Avitsena; 2002. p. 156.
Lapach SN, Chubenko AV, Babich PN. Statistical methods in biomedical research using Excel. Kyiv: Morion; 2000. p. 320.
Deryugina AV, Koryagin AS, Kopylova SV, Talamanova MN. Methods of studying the stress and adaptive reactions of the body according to blood parameters. Nizhniy Novgorod: Publishing house of Nizhny Novgorod State University; 2010. p. 25.
Khyshiktuyev BS, Khyshiktuyeva NA, Ivanov VN. Methods of determining the products of lipid peroxidation in an exhaled breath condensate and their clinical significance. Clinical laboratory diagnostics. 1996;3:13–15.
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