M. I. Marushchak, M. M. Khudobiak, I. Ya. Krynytska, I. V. Antonyshyn


Background. The injured with polytrauma are classified as extremely severe patients, as their emergency care and intensive care is followed by significant difficulties, frequent development of complications, high mortality and survivors disability.

Objective. This study is aimed to evaluate the effect of lipid peroxidation in heart, lungs, liver and blood of rats in early and late periods of combined traumatic injury of chest and hips and to establish their influence on the development of multiple organ failure.

Methods. The study was conducted on 70 adult male white nonlinear rats. It was determined the content of lipid hydroperoxides and concentration of TBA-active products in erythrocyte mass, heart, lungs and liver.

Results. The data prove activation of free radical oxidation at the first day of post-traumatic period. Analysis of the data evidences the increase in of lipid hydroperoxides (HPL) rate in liver homogenate in group E1 in 1.5 times, in all subsequent periods of the research the increase in rate fluctuated within 1.9-2.0 times. In blood and heart the HPL rate increased twice in group E1, reached maximum in group E2 and gradually decreased till the end of the experiment, data exceeded the control group. The highest level of HPL was determined in lung tissues (r≤0.01). In the post traumatic period the highest TBA-AP was detected in lungs and liver.

Conclusions. In case of simulated trauma (injury of chest with fractures of both hips) hyperactivation processes of free radical oxidation is observed in 1 day, reaching a peak in 7-14 days of post-traumatic period in blood, tissues, liver, heart and lungs if compared to the control group causing multiple organ failure.


trauma; lipid peroxidation; lung; liver; heart.

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Guriev SA, Tanasienko PV, Satsyk SP. Clinical and epidemiological characteristics of patients with infectious complications of trauma due to accidents. Medicine today and tomorrow 2012; 1: 54.

Gao JM, Gao YH, Zeng JB, Wang JB. Polytrauma with thoracic and / or abdominal injuries: experience in 1540 cases. J. Traumatol 2006; 9: 108-114.

WHO. Trauma Care Checklist [electronic resource]. - Access: index.html.

Aghajanian VV, Ustyantseva IM, Pronskiy AA. Polytrauma. Emergency care and transportation. Novosibirsk: Nauka 2008; 320.

Malysh IR, Dvorskyy PD, Zhrzheblovskaya LV, Krylyuk VA. The strategy of empirical antibiotic therapy in patients with severe polytrauma. Materials of All-Ukrainian scientific-practical conference with international participation. Zaporizhzhia 2010; 95-97.

Tsybulyak GN. Treatment of severe damage. St. Petersburg: Hyppokrat 1995; 424.

Adams HA, Trenz O. Uter Mitarbeit der IAG Schock der DIVI. Anaesthesieologie und Intensiv medizin 2007; 73–97.

Prydruha SM, Borys RM. A violation of the humoral component of immunity during the period of late manifestations of wound dystrophy and its correction by thiotriazoline. Bukovyn. Med. Visn 2013; 17 (1): 96–101.

Vlizlo VV, Fedoruk RS, Ratych IB. Laboratory methods of research in biology, animal husbandry and veterinary medicine. Lviv: Spolom, 2012. 764. (In Ukrainian).

Korobeynikova E. A modification of lipid peroxidation products assessment in the reaction with thiobarbituric acid. Lab. delo. 1989;(7):8-9. (In Russian).

lowry OH, Rosebrough NJ, Farr Al, Randall RJ. Protein measurement with the Folin VP Rosalovsky, SV Grabovska, YuT Salyha 132 ISSN 2409-4943. Ukr. Biochem. J., 2015, vol. 87, N 5 phenol reagent. J. Biol. Chem 1951; 193 (1): 265–275.

Marushchak M, Krynytska I, Petrenko N, Klishch I. The determination of correlation linkages between level of reactive oxygen species, contents of neutrophiles and blood gas composition in experimental acute lung injury. Georgian medical news. 2016; 253:98-103.

Dushianthan A, Grocott MP, Postle AD, Cusack R. Acute respiratory distress syndrome and acute lung injury. Postgrad. Med. J. 2011; 87(1031): 612–622.

Henynh TP, Kseyko DA. Indicator peroxide oxidation and antioxidant protection lipids in the system 'blood sera - erythrocytes "in acute circulation hypoxi.a Success of modern natural science 2004; 4: 17-20.

Malysh IR, Kozlov VK, Zhrzheblovskaya LV. Profile of cyrculating cytokines and their mononuclear production in dynamics in post traumatical period injuired with polytrauma. Cytokines and Inflammation 2007;6(3): 49-56.

D. Kozak Features indices of lipid peroxidation in the dynamics of early and late periods of trauma. Actual problems of emergency 2012; 3 (29): 103-106.



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