Iu. M. Ordynskyi, O. V. Denefil


Background. Cardiovascular morbidity is a topical issue; stress is an essential contributing factor. Pathogenic links in damaging stress impact on the animal units of different reactivity is promising in disease prevention and development of individual correction methods.

Objective. Stress-induced development of cardiovascular pathology is undeniable, the stress impact depending on individual systemic response, age and sex.

Methods. 96 hypoxia high- and low-resistant (HR and LR, respectively) Wistar rats aged 5.5-6 months were used in experiments. Changes of lipid peroxidation processes, as well as protein oxidative modification, nitrite anion content and the indices of antioxidant defence under immobilizing stress were studied in the research. 

Results. Immobilizing stress causes the development of oxidative and carbonyl stress in HR and LR rats that is more pronounced in LR group; and the activation of antioxidant defence system. In males, stress development is concomitant with increased catalase activity as well as that of blood peroxidase, ceruloplasmin and reduced glutathione content, whereas increased catalase and ceruloplasmin activity has been found in HR females, and that of superoxide dismutase and ceruloplasmin – in LR group. As compared with the females, more intensive oxidative and nitroxidative stress, protein oxidative modification, and stress-related accumulation of circulating immune complexes have been found in the males.

Conclusion. The most intensive oxidative and nitroxidative stress, protein oxidative modification, and stress-related accumulation of circulating immune complexes have been found in the hypoxia low-resistant males.


immobilizing stress; resistance to hypoxia; heart; damage.

Full Text:



Rodin R, Bonanno GA, Rahman N, et al. Expressive flexibility in combat veterans with posttraumatic stress disorder and depressionю J. Affect. Disord 2016; 207: 236–241.

Crea F, Battipaglia I, Andreotti F. Sex differences in mechanisms, presentation and management of ischaemic heart disease. Atherosclerosis 2015; 241 (1): 157–168.

Pimple P, Shah AJ, Rooks C, et al. Angina and mental stress-induced myocardial ischemia. J. Psychosom. Res 2015; 78 (5): 433–437.

Berezovsky VA. Hypoxia and individual features of reactivity. K.: Naukova Dumka 1978; 216. (In Russian).

Kulinskiy VI, Olkhovskiy IA. Two adaptation strategies in adverse conditions: resistant and tolerant. The role of hormones and receptors. Successes of modern biology 1992; 112: 697–711. (In Russian).

Khyshiktuyev BS, Khyshiktuyeva NA, Ivanov VN. Methods of determination of lipid peroxidation products in exhaled breath condensate and their clinical significance. Clinical Laboratory Diagnostics 1996; 3: 13–15. (In Russian).

Preclinical studies of medication: guidelines; ed. by Corr. Members of AMS of Ukraine A.V. Stefanov. K., Avicenna 2001; 528. (In Ukrainian).

Archakov AI, Mikhosoev IM. Modification of proteins by active oxygen and their decomposition. Biochemistry 1998; 54 (2): 179–185. (In Russian).

Green IC, Davie AW, Golawski J et al. Analisis of nitrate, nitrite and [15N] nitrate in biological fluids. Anal. biochem 1982; 126 (1): 131–138.

Hayevska MYu. Circulating immune complexes in the conditions of norm and pathology. Journal of scientific research 2000; 4: 37–40. (In Ukrainian).

Chevari C, Chaba I, Sokey I.The role of superoxide dismutase in cell oxidation process and the method of its determination in biological materials. Lab. bus 1985; 11: 678–681. (In Russian).

Koroljuk MA, Ivanova LI, Mayorova IG, Tokarev VE. The method of determining the activity of catalase. Lab. Bus 1988; 1: 16–19. (In Russian).

Clinical and laboratory diagnosis. Normative directive legal documents. K: Medinform 2003; 856. (In Ukrainian).

Popov T, Neykovska L. Method of determining the peroxidase activity of blood. Hygiene and sanitation 1971; 10: 89–93. (In Russian).

Moffat JA, Armstrong PW, Marks GS. Investigations into the role of sulfhydryl groups in the mechanism of action of the nitrates. Canadian Journal of Physiology and Pharmacology 1982; 60 (10): 1261–1266.

Kruhlіkova HO, Shtutman IM. Glutathione peroxidase and glutathione reductase activity of the liver of rats after introduction of sodium selinate. Ukrainian biochemical journal 1976; 2: 227–233. (In Ukrainian).

DOI: http://dx.doi.org/10.11603/ijmmr.2413-6077.2016.2.7030


  • There are currently no refbacks.