MICROBIOLOGICAL ANALYSIS OF WOUND CONTENT IN PATIENTS WITH TYPE 2 DIABETES MELLITUS WITH DIABETIC FOOT SYNDROME
DOI:
https://doi.org/10.61751/ijmmr/1.2024.06Keywords:
metabolic disease, diabetic foot infection, microorganisms, antibiotics, antibiotic resistanceAbstract
An important component of the comprehensive conservative treatment of diabetic ulcers is antibacterial therapy, as without timely and correct treatment, patients may develop toxic shock syndrome, leading to multiple organ failure. The aim of the study was to examine the species variety of the wound content in diabetic foot ulcers in patients with type 2 diabetes and to determine the susceptibility of the isolated microorganisms to antimicrobial drugs. Given the results of the bacteriological examination of the wound content, the most frequently isolated microorganisms were: Staphylococcus aureus, Staphylococcus haemolyticus, Klebsiella spp., Pseudomonas aeruginosa, Corynebacterium spp., Escherichia coli, Proteus spp., and fungi of the genus Candida spp. The study not only investigated the microbial variety in diabetic foot syndrome but also established the effectiveness of applying antibacterial agents to specific isolated pathogens. The most effective antibiotics were tigecycline and vancomycin, with the susceptibility of the specified microorganisms ranging from 89.6% to 100.0%, respectively. The susceptibility of S. aureus and S. haemolyticus to amikacin was 75.9% and 62.1%, respectively. P. aeruginosa was susceptible to only 3 out of the 12 antibiotics included in the study, namely: amikacin, vancomycin, and ciprofloxacin. Klebsiella spp. showed moderate susceptibility (45.5%) only to doxycycline. Corynebacterium spp. was resistant to most of the studied antimicrobial agents, and only to amikacin, tigecycline, vancomycin, and chloramphenicol, the susceptibility level was within 50.0-100.0%. E. coli demonstrated high susceptibility (100.0%) to such antimicrobial drugs as ciprofloxacin, ceftazidime, chloramphenicol, amikacin, azithromycin, tigecycline, and ertapenem. Proteus spp. was susceptible (100.0%) to chloramphenicol, amikacin, azithromycin, tigecycline, and ertapenem. The identification of the microbial diversity of the wound contents of a diabetic ulcer and a broad antibiotic profile will allow the optimization of antibiotic therapy in accordance with the treatment protocol for this pathology and prevent the development of antibiotic resistance
Received: 19.02.2024 | Revised: 01.05.2024 | Accepted: 28.05.2024
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