INFLUENCE OF THICK EXTRACT FROM MAITAKE MUSHROOMS ON SIGNS OF INFLAMMATORY PROCESS IN EXPERIMENTAL TOXIC HEPATITIS
DOI:
https://doi.org/10.11603/ijmmr.2413-6077.2021.1.12100Keywords:
maitake mushrooms, paracetamol, acute hepatitis, inflammationAbstract
Background. The priority of the contemporary pharmaceutical industry is to create effective, safe and inexpensive drugs to ensure the highest quality of care and optimal use of available raw materials.
Objective. The aim of our study was to investigate anti-inflammatory properties of the Maitake mushrooms thick extract in the experiment on rats with paracetamol(acetaminophen)-induced hepatitis.
Methods. 60 white male rats, weighing 180-210 g, randomized into 10 groups of 6 animals in each, were used for the experiment. Paracetamol hepatitis was simulated by acetaminophen intragastric administering in a dose of 1250 mg/kg 1 time per day (for 2 days) as a suspension in 2% starch gel solution. Maitake mushrooms thick extract, which was administered intragastrically 2 hours before the administration of acetaminophen and daily after the lesion in a dose of 150 mg/kg of the animal’s body weight, was used for the toxic lesion correction. “Silibor” was selected as the comparison drug, which was administered according to the same scheme as the investigated extract in a dose of 20 mg/kg of the animal’s body weight. Euthanasia was conducted on the 3rd, 7th and 10th day of the experiment with sodium barbamyl. Liver homogenate and animal serum were used for the studies. The development of inflammatory processes was studied by the content of pro-inflammatory and anti-inflammatory cytokines, as well as C-reactive protein in the serum of rats with toxic hepatitis and after the application of Maitake mushroom extract and the comparison drug.
Results. It was found that the introduction of acetaminophen to animals for the acute hepatitis simulation is accompanied by changes in the cytokine profile, i.e. an increase in the level of IL-6 and a decrease in the level of IL-4 in the serum of rats. Inflammatory development is evidenced by the content of C-reactive protein increase in the blood of the affected animals. The application of Maitake mushroom extract facilitated bringing the studied indicators almost to the level of intact control.
Conclusions. Reduction of inflammation signs in rats with the simulated paracetamol hepatitis under the influence of Maitake mushrooms thick extract confirms its anti-inflammatory properties.
Objective. The aim of our study was to investigate anti-inflammatory properties of the Maitake mushrooms thick extract in the experiment on rats with paracetamol(acetaminophen)-induced hepatitis.
Methods. 60 white male rats, weighing 180-210 g, randomized into 10 groups of 6 animals each, were used for the experiment. Paracetamol hepatitis was simulated by acetaminophen intragastric administering in a dose of 1250 mg/kg 1 time per day (for 2 days) as a suspension in 2% starch gel solution. Maitake mushrooms thick extract, which was administered intragastrically 2 hours before the administration of acetaminophen and daily after the lesion in a dose of 150 mg/kg of the animal’s body weight, was used for the toxic lesion correction. "Silibor" was selected as the comparison drug, which was administered according to the same scheme like the investigated extract in a dose of 20 mg/kg of the animal’s body weight. Euthanasia was conducted on the 3rd, 7th and 10th day of the experiment with sodium barbamyl using. Liver homogenate and animal serum were used for the studies. The development of inflammatory processes was studied by the content of pro- and anti-inflammatory cytokines, as well as C-reactive protein in the serum of rats with toxic hepatitis and after the application of Maitake mushroom extract and the comparison drug.
Results. It was found that the introduction of acetaminophen to animals for the acute hepatitis simulation is accompanied by changes in the cytokine profile, namely, an increase in the level of IL-6 and a decrease in the level of IL-4 in the serum of rats. The inflammatory process development is evidenced by the content of C-reactive protein increasing in the blood of affected animals. The application of Maitake mushroom extract helped to bring the studied indicators closer to the level of intact control.
Conclusions. The application of the Maitake mushrooms thick extract as a corrective factor at the simulated paracetamol hepatitis confirms its anti-inflammatory properties.
KEYWORDS: Maitake mushrooms, paracetamol, hepatitis, inflammatory processes, thick extract, anti-inflammatory properties.
References
Beger RD, Bhattacharyya S, Yang X, et al. Translational biomarkers of acetaminophen-induced acute liver injury. Arch Toxicol. 2015;89(9):1497-522.
Caparrotta TM, Antoine DJ, Dear JW.: Are some people at increased risk of paracetamol-induced liver injury? A critical review of the literature. Eur J Clin Pharmacol. 2017;74(2):147-160.
Xiao C, Wu Q, Xie Y, et al. Hypoglycemic effects of Grifola frondosa (Maitake) polysaccharides F2 and F3 through improvement insulin resistance in diabetic rats. Food and Function. 2015; Issue 11: 29.
Ji H-Y, Yu J, Liu A. Structural characterization of a low molecular weight polysaccharide from Grifola frondosa and its antitumor activity in H22 tumor-bearing mice. Journal of Functional Foods. 2019;V.61:10342.
DOI: 10.1016/j.jff.2019.103472
Herasymets II, Fira LS, Medvid II. Study of the conditionally therapeutic dose of thick extract from Maitake mushrooms. Сolloquium-journal. 2020; 13(65):5-9.
DOI: 10.24411/2520-6990-2020-11853.
Li Q, Zhang F, Chen G, et al. Purification, characterization and immunomodulatory activity of a novel polysaccharide from Grifola frondosa. Int J Biol Macromol. 2018;V.111:1293-1303.
Xiang Q, Zhang W, Li Q, et al. Investigation of the uptake and transport of polysaccharide from Se-enriched Grifola frondosa in Caco-2 cells model. Int J Biol Macromol. 2020;V.158:1330-1341.
Ma X, Zhou F, Chen Y, et al. A polysaccharide from Grifola frondosa relieves insulin resistance of HepG2 cell by Akt-GSK-3 pathway. Glycoconjugate Journal. 2014;31:335-363.
Zhang Y, Sun D, Meng Q, et al. Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway. International journal of molecular medicine. 2017; 40(4):1089-1095.
Gross D, Tolba R. Ethics in Animal-Based Research. Eur Surg Res. 2015;55(1-2):43-57.
Stefanov AV.: Preclinical trials of medicines. Guidelines: under. ed. Corresponding Member AMS of Ukraine AV Stefanov. К: Avitsenna. 2002;568.
Vashkeba-Bitler ЕМ. Determination of anti-inflammatory and antimicrobial activity of the extract from the aerial part of horseradish. Pharmaceutical Review. 2014;4:122-124.
Rybolovlev YR, Rybolovlev RS. Dosing of substances for mammals according to the constants of biological activity. Reports of the USSR Academy of Sciences. 1979;Т.247,6:1513-1516.
Zhou B, Shu B, Yang J, et al. C-reactive protein, interleukin-6 and the risk of colorectal cancer: a meta-analysis. Cancer Causes Control. 2014; 25(10): 1397–1405.
Altynbaeva YI, Teplova SN. Cell damage markers, cytokines and terminal stable metabolites of nitric oxide in saliva in smoking patients in the early stages of chronic obstructive pulmonary disease. Cytokines and inflammation, 2011;4:15-22.
Alybaeva KM, Berdyyarova NA, Mukhamedzhanova NK, et al. Analysis of the quantitative determination of the level of C-reactive protein and procalcitonin in patients with infectious diseases. Bulletin of AGIUV. 2015;1-2:36-40.
Jannot AS, Agoritsas T, Gayet-Ageron A, et al. Citation bias favoring statistically significant studies was present in medical research. J Clin Epidemiol. 2013; 66(3): 296-301.
DOI: 10.1016/j.jclinepi.2012.09.015.
Shelamova MA, Insarova NI, Lieshchienko VH. Statistical analysis of medical and biological data using the EXCEL program. Minsk, BGMU. 2010; 96.
Zhang X, Liu S, Zhou Y.: Circulating levels of C-reactive protein, interleukin-6 and tumor necrosis factor-α and risk of colorectal adenomas: a meta-analysis. Oncotarget. 2016; 27, 7(39): 64371–64379.
Heikkilä K, Harris R, Lowe G, et al.: Associations of circulating C-reactive protein and interleukin-6 with cancer risk: findings from two prospective cohorts and a meta-analysis. Cancer Causes Control. 2009;20:15–26.
DOI: 10.1007/s10552-008-9212-z.
Nam J, Park K, Park E, et al.: Interleukin-13/-4-induced oxidative stress contributes to death of hippocampal neurons in aβ1-42-treated hippocampus in vivo. Antioxid Redox Signal. 2012;16(12):1369-83.
DOI: 10.1089/ars.2011.4175.
Gaber W, Azkalany GS, Gheita TA.: Clinical significance of serum interleukin-6 and -174 G/C promoter polymorphism in Rheumatoid arthritis patients. Egypt Rheumatol. 2013;35(2):107-113.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 International Journal of Medicine and Medical Research
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who sent their manuscript to International Journal of Medicine and Medical Research agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License CC-BY-NC that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
2. Authors able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
