infertility, endometriosis, hormones, sclerotherapy, inositol, vitamin D3


Background. Endometriosis-associated infertility (EAI) has a number of specific features, which are crucial in the choice of medical treatment.

Objective. The aim of the study is to analyze endocrine profile in women with EAI before and after sclerotherapy and pregravid preparation (PP), which includes a vitamin complex FT 500 plus with inositol and vitamin D3.

Methods. The study involved 70 women aged 21-40 years with endometriosis-associated infertility. The comparison group included 30 women with tuboperitoneal infertility. ELISA was used to determine concentrations of Anti-Mullerian hormone (AMH), follicle stimulating hormone (FSH), luteinizing hormone (LH), progesterone and estradiol in blood serum using a standard kit by Diagnostic Systems Laboratories, Inc (USA). During two menstrual cycles the FT 500 plus was prescribed once a day from the 2nd/3rd day of the cycle, vitamin D3 was prescribed at the dose of 2,000 IU for women without its deficiency and in therapeutic doses in case of hypovitaminosis. The sclerotherapy with 95% ethanol solution was performed on the 6th-8th day of menstrual cycle.

Results. It was established that in women with EAI undergoing PP and sclerotherapy the level of AMH was lower (by 12.90%) as well as progesterone (by 9.84%), while FSH (by 14.47%), LH (by 21.14%) and estradiol (by 35.55%) was higher compare to the comparison group. At the same time, FSH (by 21.98%), LH (by 32.89%) and estradiol (by 32.23%) concentrations were significantly lower compare to their primary indices before sclerotherapy.

Conclusions. Sclerotherapy and PP with a vitamin complex, inositol and vitamin D3 has a positive effect on endocrine profile in women with endometriosis-associated infertility

Author Biographies


PhD student, Department of Obstetrics and Gynecology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine


Professor of the Department of Obstetrics and Gynecology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine


Abbas S, Ihle P, Koster I, Schubert I. Prevalence and incidence of diagnosed endometriosis and risk of endometriosis in patients with endometriosis-related symptoms: findings from a statutory health insurance-based cohort in Germany. Eur J Obstet Gynecol Reprod Biol 2012;160:79–83.

Giudice LC. Clinical Practice: Endometriosis. N Engl J Med 2010; 362: 2389-98.

DOI: https://doi.org/10.1056/NEJMcp1000274

Bulun SE. Endometriosis. N Engl J Med 2009;360(3): 268-79.

DOI: https://doi.org/10.1056/NEJMra0804690

Shestakova IG, Ipastova ID. [Endometriosis: a new consensus — new solutions: Global consensus on the management of patients with endometriosis as a first step towards the creation of industry standards: News bulletin]. Moscow: Status Praesens, 2014; 16 p. (In Russian).

Shcherbyna NA, Demidenko DI, Demidenko AD. [Melatonin’s tocolytic activity in threatening preterm birth]. Emergency Medicine. 2014;5:100-102. (In Russian).

Borham MM. Comparison between omen­toplasty and partial cystectomy and drainage (PCD) techenques in surgical management of hydatid cysts liver in endemic area (Yemen). J. Egypt. Soc. Parasitol. (JESP) 2014; 44(1):145-50.

DOI: https://doi.org/10.12816/0006454

Shakiba K, Bena JF, M McGill K. Surgical treat­ment of endometriosis: a 7-year follow-up on the requirement for further surgery. Obstetrics and Gynecology. 2008;111(6):1285-92.


Khamoshina MB, Vakhabova MI, Kalinina EA. [Pharmacotherapy of endometriosis: opportunities and perspectives]. Meditsnskyi Sovet. 2013;8: 23-7. (In Russian).

Matsuzaki S, Houlle C, Darcha C. Analysis of risk factors for the removal of normal ovarian tissue during laparoscopic cystectomy for ovarian endo­metriosis. Human Reproduction. 2009; 24(6):1402-06.

DOI: https://doi.org/10.1093/humrep/dep043

Singh AK, Chattopadhyay R, Chakravarty B, Chaudhury K. Markers of oxidative stress in follicular fluid of women with endometriosis and tubal infertility undergoing IVF. Reprod Toxicol. 2013; 42:116-24.

DOI: https://doi.org/10.1016/j.reprotox.2013. 08.005

Augoulea A, Mastorakos G, Lambrinoudaki I, Christodoulakos G, Creatsas G. The role of the oxi­dative-stress in the endometriosis-related infertility. Gynecol Endocrinol.2009; 25: 75-81.

DOI: https://doi.org/10.3917/eres.strei.2009. 01.0075

Rubod C, Jean Dit Gautier E, Yazbeck C. Surgical management of endometrioma: Different alternatives in term of pain, fertility and recurrence. CNGOF-HAS Endometriosis Guidelines. Gynecol Obstet Fertil Senol. 2018 Mar;46(3):278–89.

Miquel L, Preaubert L, Gnisci A, Netter A, Courbiere B, Agostini. Transvaginal ethanol scle­rotherapy for an endometrioma in 10 steps. Fertility and Sterility. 2020.Oct;115(1):259-64.

DOI: https://doi.org/10.1016/j.fertnstert. 2020.08.1422

Gatta G, Parlato V, Di Grezia G, Porto A, Cappabianca S, Grassi R. Ultrasound-guided aspi­ration and ethanol sclerotherapy for treating endo­metrial cysts. Radiol Med.Torino. 2010 Dec;115(8): 1330–9.

DOI: https://doi.org/10.1007/s11547-010-0586-0

Aflatoonian A, Rahmani E, Rahsepar M. Assessing the efficacy of aspiration and ethanol injection in recurrent endometrioma before IVF cycle: A randomized clinical trial. Iran J Reprod Med. 2013 Mar;11(3):179–84.

Fisch JD, Sher G. Sclerotherapy with 5% tetracycline is a simple alternative to potentially complex surgical treatment of ovarian endo­met­riomas before in vitro fertilization. Fertil Steril. 2004;82(2):437-41.

DOI: https://doi.org/10.1016/j.fertnstert.2004. 01.031

Tsoumpou I, Kyrgiou M, Gelbaya TA, Nardo LG. The effect of surgical treatment for endometrioma on in vitro fertilization outcomes: a systematic review and meta-analysis. Fertil Steril. 2009;92(1):75-87.

DOI: https://doi.org/10.1016/j.fertnstert.2008. 05.049

André GM, Vilarino FL, Christofolini DM, Bianco B, Barbosa CP. Aspiration and ethanol scle­rotherapy to treat recurrent ovarian endometriomas prior to in vitro fertilization–a pilot study. Einstein (Sao Paulo). 2011 Dec;9(4):494-8.

DOI: https://doi.org/10.1590/s1679-45082011ao2081

Wang XT, Meng B, Liu JF, Jin FX and Xu H. The influences of ultrasound guided radiofrequency ablation assisted by polyol on fibroid volume and endocrine function among patients with uterine fibroid. Modern Practical Medicine 2018; 30: 1425-28.


Alborzi S, Namavar Jahromi B, Ahmadbeigi M. Recovery Rate of Patients with Recurrent Ovarian Endometriomas Using Sclerotherapy with 95% Ethanol. Journal of Obstetrics, Gynecology and Cancer Research (JOGCR). 2018 Sep 10;3(3):105-10.

DOI: https://doi.org/10.1002/epi4.12285

Khmil SV, Kulyk II, Mykula RP. [Antioxidant therapy in women with infertility caused by endo­metriosis]. Bulletin of social hygiene and health care organization. 2018; 4 (78):56-63 (In Ukrainian).

DOI: https://doi.org/10.11603/1681-2786.2018. 4.10031

Tsysar Yv, Andriets OA. [The influence of pathology of thyroid gland on menstrual function in girls of pubertal age]. Bukovinian Medical Bulletin. 2011;15(2):130–4. (In Ukrainian).




How to Cite

Kulyk, I. I., & Khmil, S. V. (2021). ENDOMETRIOSIS-ASSOCIATED INFERTILITY: THE ROLE OF HORMONES AND ITS CORRECTION. International Journal of Medicine and Medical Research, 6(2), 5–10. https://doi.org/10.11603/ijmmr.2413-6077.2020.2.12011