DIAGNOSTIC AND PROGNOSTIC SIGNIFICANCE OF MICROBIAL FLORA IMBALANCE IN GINGIVAL BIOFILM

Keywords: biofilms, microflora, gingivitis, risk of morbidity

Abstract

Background. Periodontal tissues inflammatory diseases are widespread among young people.

Objective. This study was aimed at elaborating the method to assess risks of periodontal inflammatory diseases and determining its efficacy depending on the state of dental tissues, gum tissues and sex.         

Methods. The study included 182 students (93 men, 89 women) aged 19-29: 22 individuals had no lesions of hard dental tissues and no signs of periodontal disease; 51 individuals were found to have DMF index <6;
52 individuals – DMF index ≥6; 57 individuals were diagnosed with chronic catarrhal gingivitis. Primary groups were formed in autumn; re-examination was carried in spring. The research participants were assessed for detection of risks of periodontal inflammatory disease by the method developes by the authors (Patent UA 54041).

Results. The study revealed that the risk of development of preiodontitis increases in individuals with high caries and gingivitis intensity. In spring, more individuals suffer from microbial imbalance in in the composition of gingival sulcus fluid and decrease in the mean stability coefficient value that indicates an increased risk of inflammatory periodontal disease development. Women were less likely to experience seasonal dysbiotic changes in the gingival sulcus fluid composition compared with men.

Conclusions. The method suggested for assessment of the risk of periodontal inflammatory diseases is of high informativeness. It allows clinicians detecting early pre-nosological signs of oral microbiocenosis imbalance that enhances the effectiveness of early diagnosis of inflammatory periodontal diseases.

Author Biographies

G. A. Loban, UKRAINIAN MEDICAL STOMATOLOGICAL ACADEMY, POLTAVA, UKRAINE

доктор медичних наук, професор, завідувачка кафедри мікробіології, вірусології та імунології Української медичної стоматологічної академії, Полтава, Україна

T. O. Petrushanko, UKRAINIAN MEDICAL STOMATOLOGICAL ACADEMY, POLTAVA, UKRAINE

MD, Ph.D., DSc, Head of Therapeutic Dentistry Department, Ukrainian Medical Stomatological Academy, Poltava, Ukraine

V. V. Chereda, UKRAINIAN MEDICAL STOMATOLOGICAL ACADEMY, POLTAVA, UKRAINE

PhD, Lecturer of Therapeutic Dentistry Department, Ukrainian Medical Stomatological Academy, Poltava, Ukraine

M. O. Faustova, UKRAINIAN MEDICAL STOMATOLOGICAL ACADEMY, POLTAVA, UKRAINE

PhD, Lecturer of Microbiology, Virology and Immunology Department, Ukrainian Medical Stomatological Academy, Poltava, Ukraine

M. M. Ananieva, UKRAINIAN MEDICAL STOMATOLOGICAL ACADEMY, POLTAVA, UKRAINE

MD, PhD, Associate Professor of Microbiology, Virology and Immunology Department, Ukrainian Medical Stomatological Academy, Poltava, Ukraine

Ya. A. Basarab, UKRAINIAN MEDICAL STOMATOLOGICAL ACADEMY, POLTAVA, UKRAINE

MD, Lecturer of Microbiology, Virology and Immunology Department, Ukrainian Medical Stomatological Academy, Poltava, Ukraine

References

Kassebaum NJ, Smith AG, Bernabé E, Fle­ming TD, Reynolds AE, Vos T et al. GBD 2015 Oral Health Collaborators. Global, regional, and national preva­lence, incidence, and disability-adjusted life years for oral conditions for 195 countries, 1990-2015: a syste­matic analysis for the global burden of diseases, inju­ries, and risk factors. J Dent Res. 2017; 96,4:380-7.

doi: 10.1177/0022034517693566

Machado V, Botelho J, Amaral A, Proença L, Alves R, Rua J et al. Prevalence and extent of chronic periodontitis and its risk factors in a Portuguese subpopulation: a retrospective cross-sectional study and analysis of Clinical Attachment Loss. PeerJ. 2018; 24, 6, e5258.

doi: 10.7717/peerj.5258. eCollection 2018

Oppermann RV, Haas AN, Rösing CK, Susin C. Epidemiology of periodontal diseases in adults from Latin America. Periodontol 2000, 2015; 67, 1: 13-33.

doi: 10.1111/prd.12061

Nazarchuk ОА, Paliy VG, Bereza ВМ, Yatsula OV, Zaderey NV, Gonchar OO, et al. The research of qualities of microflora from tooth-gingival sulcus in patients with gingivitis. Herald of Vinnytsia National Medical University. 2016; 2:370-375 [In Ukrainian].

Lof M, Janus MM, Krom BP. Metabolic Inte­ractions between Bacteria and Fungi in Commensal Oral Biofilms. J Fungi (Basel). 2017;14,3,3, pii: E40.

doi: 10.3390/jof3030040

Petrushanko TA, Chereda VV, Loban' GA. The relationship between colonization resistance of the oral cavity and individual-typological characteristics of personality: dental aspects. Wiad Lek. 2017;70,4: 754-7.

Rosier BT, Marsh PD, Mira A. Resilience of the Oral Microbiota in Health: Mechanisms That Prevent Dysbiosis. J Dent Res. 2018;97,4:371-80.

doi: 10.1177/0022034517742139

Faustova MO, Ananieva MM, Basarab YO, Dobrobolska OV, Vovk IM, Loban' GA. Bacterial factors of cariogenicity (literature review). Wiad Lek. 2018; 71,2: 378-82.

WMA declaration of helsinki – ethical principles for medical research involving human subjects. https://www.wma.net/policies-post/wma-declara­tion-of-helsinki-ethical-principles-for-medical-research-involving-human-subjects/

Chereda VV, Petrushanko TO, Loban' GA. A method for assessing the risk of inflammatory periodontal disease. Health Newsletter. Kyiv: Ukrmedpatentinform Ministry of Health of Ukraine. 2012;21,136:3. [In Ukrainian].

Ebersole JL, Dawson D, Emecen-Huja P, Nagarajan R, Howard K, Grady ME, et al. The periodontal war: microbes and immunity. Periodontol 2000. 2017;75,1:52-115.

doi: 10.1111/prd.12222

Herrero ER, Fernandes S, Verspecht T, Ugarte-Berzal E, Boon N, Proost P et al. Dysbiotic Biofilms Deregulate the Periodontal Inflammatory Response. J Dent Res. 2018; 97,5:547-55.

doi: 10.1177/0022034517752675

Rafiei M, Kiani F, Sayehmiri K, Sayehmiri F, Tavirani M et al. Prevalence of Anaerobic Bacteria (P.gingivalis) as Major Microbial Agent in the Incidence Periodontal Diseases by Meta-analysis. J Dent (Shiraz). 2018; 19,3:232-42.

Ananieva MM, Faustova MO, Basarab IO, Loban' GA. Kocuria rosea, Kocuria kristinae, Leuco­nostoc mesenteroides as caries-causing representa­tives of oral microflora. Wiad Lek. 2017;70,2,2:296-8.

Ananieva M, Nazarchuk O, Faustova M, Basarab Y, Loban G. Pathogenicity factors of kocuria kristinae contributing to the development of peri-implant mucositis. Mal J Med Health Sci. 2018;14,3: 34-8.

Published
2020-02-18
How to Cite
Loban, G. A., Petrushanko, T. O., Chereda, V. V., Faustova, M. O., Ananieva, M. M., & Basarab, Y. A. (2020). DIAGNOSTIC AND PROGNOSTIC SIGNIFICANCE OF MICROBIAL FLORA IMBALANCE IN GINGIVAL BIOFILM. International Journal of Medicine and Medical Research, 5(2), 76-82. https://doi.org/10.11603/ijmmr.2413-6077.2019.2.10448