Exploring water disinfection through electrolytic ozonation for application in wartime conditions

Authors

DOI:

https://doi.org/10.61751/bmbr/1.2024.43

Keywords:

ozone, water disinfection, inactivation of microorganisms, portable ozone generator, armed conflict

Abstract

Clean and safe water is a fundamental requirement for human survival and well-being. The destruction of civilian
infrastructure during wars or natural disasters can severely limit access to clean water for the local population, military,
rescue workers and volunteers. The objective of the study was the evaluation of the efficiency of fresh water disinfection by
electrolytic ozonation. The ozone concentration was measured photometrically by the intensity of the colour change in the
reaction with N, N-diethyl-p-phenylenediamine sulfate. The water quality was assessed microbiologically by determining
the total microbial count of the samples and by analysing the colour of the bottom of the flasks in which the water samples
were stored. The efficiency of ozonation of artesian water in the field was evaluated. Electrolytic ozonation of 500 mL of
spring water for two and five minutes produced an aqueous ozone solution with a concentration of 0.74 and 1.72 mg/L,
respectively. Electrolytic ozone decomposed slowly and was detected in the samples three days after the water treatment.
Ozonation of spring water for 5 and 10 minutes effectively inactivated all natural microorganisms (2.11 log CFU/mL),
the water remained sterile and without any colour changes for a month. Water samples ozonated for 2 minutes did not
demonstrate any visible changes after a month of storage, however the natural microflora partially recovered. Planktonic
microorganisms in the amount of 2.33 log CFU/mL, as well as biofilms on the walls of the flasks, were detected in a month
in the control water samples. The colour of the bottom of the control flasks changed to dirty yellow. In the field, ozonation
of water for 10 minutes prevented its spoilage even when the recommended treatment volume was increased by 10 times.
The obtained results indicate that electrolytic ozonation is an effective method of freshwater disinfection, and portable
ozonators can play an important role in emergency water purification in areas of armed conflicts or natural disasters

Received: 07.11.2023 | Revised: 18.01.2024 | Accepted: 27.02.2024

Author Biographies

Taras Pyatkovskyy, I. Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

PhD in Medical Sciences, Associate Professor 46001, 1 Maidan Voli, Ternopil, Ukraine

Olena Pokryshko, I. Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

PhD in Medical Sciences, Associate Professor 46001, 1 Maidan Voli, Ternopil, Ukraine

Serhii Danylkov, Bogomolets National Medical University

PhD in Medical Sciences, Assistant Professor 01601, 13 Taras Shevchenko Blvd, Kyiv, Ukraine

References

Pereira P, Bašić F, Bogunovic I, Barcelo D. Russian-Ukrainian war impacts the total environment. Sci Total Environ. 2022;837:155865. DOI: 10.1016/j.scitotenv.2022.155865

Shumilova O, Tockner K, Sukhodolov A, Khilchevskyi V, De Meester L, Stepanenko S, et al. Impact of the Russia–Ukraine armed conflict on water resources and water infrastructure. Nat Sustain. 2023;6:578–86. DOI: 10.1038/s41893-023-01068-x

Cahoon LB. Water purification: Treatment of microbial contamination. In: Advances in water purification techniques: Meeting the needs of developed and developing countries. San Diego: Elsevier Inc.; 2019. Chapter 15; p. 385–95.DOI: 10.1016/B978-0-12-814790-0.00015-6

Lindmark M, Cherukumilli K, Crider YS, Marcenac P, Lozier M, Voth-Gaeddert L, et al. Passive in-line chlorination for drinking water disinfection: A critical review. Environ Sci Technol. 2022;56(13):9164–81. DOI: 10.1021/acs.est.1c08580

Pandiselvam R, Subhashini S, Banuu Priya EP, Kothakota A, Ramesh SV, Shahir S. Ozone based food preservation: A promising green technology for enhanced food safety. Ozone Sci Eng. 2019;41(1):17–34. DOI: 10.1080/01919512.2018.1490636

Zheng D, Huang J, Fang Y, Deng Y, Peng C, Dehaen W. Fluorescent probes for ozone-specific recognition: An historical overview and future perspectives. Trends Environ Anal Chem. 2023;38:e00201. DOI: 10.1016/j.teac.2023.e00201

Chuwa C, Vaidya D, Kathuria D, Gautam S, Sharma S, Sharma B. Ozone (O3): An emerging technology in the food industry. Food Nutr J. 2020;5:224. DOI: 10.29011/2575-7091.100124

Buntasana S, Seankongsuk P, Vilaivan T, Padungros P. Household ozone disinfector as an alternative ozone generator for ozonolysis of alkenes. Am J Org Chem. 2021;10(5):1141–52. DOI: 10.1002/ajoc.202100024

Grignani E, Mansi A, Cabella R, Castellano P, Tirabasso A, Sisto R, et al. Safe and effective use of ozone as air and surface disinfectant in the conjuncture of COVID-19. Gases. 2021;1(1):19–32. DOI: 10.3390/gases1010002

Li X, Ma J, He H. Recent advances in catalytic decomposition of ozone. J Environ Sci. 2020;94:14– DOI: 10.1016/j.jes.2020.03.058

Epelle EI, Macfarlane A, Cusack M, Burns A, Amaeze N, Richardson K, et al. Stabilisation of ozone in water for microbial disinfection. Environments. 2022;9(4):45. DOI: 10.3390/environments9040045

Pyatkovskyy TI, Pokryshko OV, Danylkov SO. Assessing the effectiveness of ozonation for fresh water disinfection. Achiev Clin Exp Med. 2023;(4):113–18. DOI: 10.11603/1811-2471.2023.v.i4.14189

Okada F, Nay K. Electrolysis for ozone water production. In: Linkov V, Kleperis J, editors. Electrolysis. London: IntechOpen; 2012. P. 243–72. DOI: 10.5772/51945

Kepler G. Image color extract [Internet]. [cited 2024 Jan 23]. Available from: http://www.coolphptools.com/color_extract

UNESCO. Universal Declaration on Bioethics and Human Rights [Internet]. [cited 2024 Jan 23]. Available from: https://www.unesco.org/en/legal-affairs/universal-declaration-bioethics-and-human-rights?hub=66535

Vu HP, Nguyen LN, Zdarta J, Nga TTV, Nghiem LD. Blue-green algae in surface water: Problems and opportunities. Curr Pollut Rep. 2020;6:105–22. DOI: 10.1007/s40726-020-00140-w

Shevchenko OL, Bezrodnyi DA. Artificial salinization of the aquifer in Poltava sequence deposits in the village of Tarasivka, Kyiv region. Geochem Technog. 2020;4:40–49. DOI:10.15407/geotech2020.32.040

Hirahara Y, Iwata K, Nakamuro K. Effect of citric acid on prolonging the half-life of dissolved ozone in water. Food Saf. 2019;7(4):90–94. DOI: 10.14252/foodsafetyfscj.D-19-00005

Ziyaina M, Rasco B. Inactivation of microbes by ozone in the food industry: A review. Afr J Food Sci. 2021;15(3):113–20. DOI: 10.5897/AJFS2020.2074

Varol K. Determination of maximum ozone concentrations and half-life of ozone in some drinking water and high-quality pure water produced in new system devices. Fresenius Environ Bull. 2022;31:2285–94.

Seki M, Ishikawa T, Terada H, Nashimoto M. Microbicidal Effects of stored aqueous ozone solution generated by nano-bubble technology. In Vivo. 2017;31(4):579–83. DOI: 10.21873/invivo.11097

Meunier L, Canonica S, von Gunten U. Implications of sequential use of UV and ozone for drinking water quality. Water Res. 2006;40(9):1864–76. DOI: 10.1016/j.watres.2006.02.030

Gorito AM, Pesqueira JFJR, Moreira NFF, Ribeiro AR, Pereira MFR, Nunes OC, Almeida CMR, Silva AMT. Ozone-based water treatment (O3, O3/UV, O3/H2O2) for removal of organic micropollutants, bacteria inactivation and regrowth prevention. J Environ Chem Eng. 2021;9(4):105315. DOI: 10.1016/j.jece.2021.105315

Zhang T, Tao YZ, Yang HW, Chen Z, Wang XM, Xie YF. Study on the removal of aesthetic indicators by ozone during advanced treatment of water reuse. J Water Process Eng. 2020;36:101381. DOI: 10.1016/j.jwpe.2020.101381

Ren X, Wu Q, Shu J, Chen C, Tiraferri A, Liu B. Efficient removal of organic matters and typical odor substances in rural drinking water using Ozone-BAC-UF combined system to meet new water quality standards in China. Sep Purif Technol. 2023;327:124899. DOI: 10.1016/j.seppur.2023.124899

Tripathi S, Hussain T. Water and wastewater treatment through ozone-based technologies. In: Development in Wastewater treatment research and processes: Removal of emerging contaminants from wastewater through bio-nanotechnology. Amsterdam: Elsevier; 2022. Chapter 7; p. 139–72. DOI: 10.1016/B978-0-323-85583-9.00015-6

Manasfi T. Ozonation in drinking water treatment: an overview of general and practical aspects, mechanisms, kinetics, and byproduct formation. Comprehensive Anal Chem. 2021;92:85–16. DOI: 10.1016/bs.coac.2021.02.003

Marino M, Maifreni M, Baggio A, Innocente N. Inactivation of foodborne bacteria biofilms by aqueous and gaseous ozone. Front Microbiol. 2018;9:2024. DOI: 10.3389/fmicb.2018.02024

Oliver JC, Bredarioli PAP, Leandro FD, Ferreira CBRJ, Veiga SMOM, Dias ALT. Ozone against Pseudomonas aeruginosa biofilms in contact lenses storage cases. Rev Inst Med Trop Sao Paulo. 2019;61:e23. DOI: 10.1590/S1678-9946201961023

Zhang L, Graham N, Li G, Zhu Y, Yu W. Excessive ozonation stress triggers severe membrane biofilm accumulation and fouling. Environ Sci Technol. 2024;58(13):5899–10. DOI: 10.1021/acs.est.3c10429

Hendrickson C, Oremo J, Akello OO, Bunde S, Rayola I, Akello D, et al. Decentralized solar-powered drinking water ozonation in Western Kenya: An evaluation of disinfection efficacy. Gates Open Res. 2020;4:56. DOI: 10.12688/gatesopenres.13138.2

Hafeez A, Shamair Z, Shezad N, Javed F, Fazal T, Rehman Su, Bazmi AA, Rehman F. Solar powered decentralized water systems: A cleaner solution of the industrial wastewater treatment and clean drinking water supply challenges. J Clean Prod. 2021;289:125717. DOI: 10.1016/j.jclepro.2020.125717

Downloads

Published

2024-03-21

How to Cite

Pyatkovskyy, T., Pokryshko, O., & Danylkov, S. (2024). Exploring water disinfection through electrolytic ozonation for application in wartime conditions. Bulletin of Medical and Biological Research, (1), 43–51. https://doi.org/10.61751/bmbr/1.2024.43