Comparative analysis of biochemical changes at the systemic level in acute ischemia-reperfusion with morphological disorders of the soft tissues of the hind limbs of rats using neural network clustering

Abstract

Summary. Ischemia-reperfusion syndrome causes biochemical disorders and changes in the pro- and antioxidant system, both at the local and systemic levels. It is important to optimize the prediction of morphological disorders in relation to biochemical changes.

The aim of the study – to establish the relationship between the state of lipid peroxidation and antioxidant protection at the systemic level and the degree of morphological changes in the soft tissues of the hind limbs of ruts under the development of ischemic-perfusion syndrome using neural network clustering.

Materials and Methods. A biochemical study of the serum of 30 white rats was performed. There were 6 intact animals in the control group. Ischemia was modeled by applying SWAT rubber tourniquets on the hind limbs of the animal, at the level of the inguinal folds, for 2 hours, and reperfusion - by removing the tourniquet and restoring blood circulation 2 hours after its application. Observations of animals were carried out by train for 14 days. Blood for the study was isolated from the apex of the heart of the experimental animals by cardiotomy.

Results. It was found that in the simulation of ischemia and reperfusion the largest deviations of the studied parameters are observed 1 day after removal of the tourniquet, namely a statistically significant increase in creatinine by 28.16 % (p<0.005), total bilirubin by 20.75 % (p<0.05), ALT by 96.14 % (p<0.005), AST by 147.27 % (p<0.005) and alkaline phosphatase by 70.84 % (p<0.005). After 14 days, most serum biochemical parameters approached values close to the control group, however, levels of creatinine, total bilirubin, ALT, AST and alkaline phosphatase activity remained statistically significantly higher than the control group by 9.65 % (p<0.005), 28.96 % (p˂0.05), 21.93 % (p˂0.05), 34.9 % (p˂0.05) and 47.84 % (p˂0.005), respectively. The value of the antioxidant-prooxidant index (API) in all terms of the study was statistically lower than the control group. In the late reperfusion period, there was a tendency to increase, however, this figure at the end of 14 days still remained lower than the group of intact animals by 26.4 % (p<0.05). In order to establish the value of combined changes of certain parameters to predict the severity of morphological disorders in different periods after removal of the turnstile in the development of ischemic-reperfusion syndrome used neural network clustering of biochemical parameters and severity of morphological disorders.

Conclusions. Ischemia-reperfusion is accompanied by significant changes in the biochemical parameters of blood serum in the first hours after the restoration of blood circulation, which indicates an increased load on the organs of the homeostasis system and on the systemic lesions. Analysis of cluster portraits in neural network clustering based on biochemical parameters of serum of white rats in combination with a parameter of the severity of morphological changes in different periods after removal of the tourniquet, found that in predicting the progression of structural changes in extremities the combined changes in creatinine, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), triene-conjugates and TBARS levels are the most important.