ANALYSIS OF LIPOPHILIC FRACTION OF THE COMMON PUSSYTOES HERB
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v0.i1.9995Keywords:
common pussytoes, lipophilic fraction, carotenoids, chlorophyllsAbstract
Introduction. The lipophilic fractions of many medicinal plants today are poorly understood, despite the fact that they contain such important biologically active substances as chlorophylls, carotenoids, tocopherols, phytosterols, fatty acids, phospholipids, etc., which according to the literature show reparative, anti-inflammatory, antiseptic, immunostimulant, antitumor activity. Compounds of lipophilic nature are carotenoids and chlorophylls are of great importance. Carotenoids increase the body's resistance to mutagens and carcinogens, reduce age-related degenerative changes in tissues, inhibit proliferation of malignant cells, activate the synthesis of cytokines and interleukins, participate in the regulation of gene transcription, and also exhibit immunomodulatory effects. The antioxidant properties of carotenoids cause their photo-protective, radioprotective, anti-mutagenic and anti-carcinogenic effects. Chlorophyll in the human body promotes the formation of hemoglobin and can be used in medicine as a valuable therapeutic agent that accelerates the processes of hematopoiesis, affects the blood's formula, as well as stabilizes the functioning of the circulatory system. It has the ability to activate various functions of the human body, in particular thyroid gland, antitoxic function of the liver, as well as a tonic, normalizes metabolic processes and improves immunity.
The aim of the study – to obtain a lipophilic extract from the herb of the common pussytoes, to study its qualitative composition and to determine the quantitative content of carotenoids and chlorophylls.
Research Methods. The lipophilic fraction from the common pussytoes herb was maintained by the way of exhausting extraction of the raw materials chloroform in the Sockslet apparatus. The obtained lipophilic fraction was used for the determination of chlorophylls and carotenoids. The definitions are followed by the TLC method. Determination of the quantitative content of chlorophylls and carotenoids was carried out by spectrophotometric method. Content of the amount of chlorophylls was determined at 670 nm in terms of chlorophyll A; carotinoids – for the benefit of a wave of 450 nm, based on β-carotene.
Results and Discussion. The results of the studies showed that the yield of the lipophilic fractions of the common pussytoes herb was equal to the absolute dry material (6.17±0.12) %. Lipophilic fraction of the common pussytoes herb is a thick butter indiscrete mass of dirty-green color with a pleasant specific odor; practically insoluble in water purified P and ethanol 96 % P, readily soluble in chlorophyll P.
The content of chlorophylls and carotenoids in the investigated extract was (0.085±0.003) % and (0.029±0.001) %. The content of chlorophylls in the common pussycat herb was 2.9 times higher than carotenoids.
Conclusions. The lipophilic fraction of the common pussytoes herb was obtained, the output of which was 6.17 %. In the lipophilic fraction, the quantitative content of chlorophylls and carotenoids was determined, which was in the common pussytoes herb 0.085 % and 0.029 %, respectively.
References
Harna, S.V., Vetrov, P.P., Rusynov, O.I., & Heorhiiants, V.A. (2011). Optymizatsiia tekhnolohii ekstraktsii lipofilnykh kompleksiv z likarskoi roslynnoi syrovyny. 1. Vybir ekstrahentu [Optimization of the technology of extraction of lipophilic complexes from the medicinal plant raw materials. 1. Selection of extractant]. Zaporozhskiy meditsinskiy zhurnal – Zaporizhzhia Medical Journal, 12 (3), 92-94 [in Ukrainian].
Kovalev, S.V. (2013). Khimicheskoe issledovanie lipofilnoy fraktsii travy lyutserny serpovidnoy [Chemical study of the lipophilic fraction of alfalfa sickle grass]. Zaporozhskiy meditsinskiy zhurnal – Zaporizhzhia Medical Journal, 3 (78), 94-97 [in Russian].
Marchyshyn, S.M., & Ambroziuk, O.B. (2011). Doslidzhennia lipofilnoi fraktsii travy perstachu husiachoho (Potentilla anserina L.) [Investigation of the lipophilic fraction of the silverweed cinquefoil herb (Potentilla anserina L.)]. Medychna khimiia – Medical Chemistry, 13 (3), 96-99 [in Ukrainian].
Harnyk, T.P., Vikhtinskaya, I.L., & Isakova, T.I. (1998). Obzor ofitsialnogo i perspektivnogo lekarstvennogo rastitelnogo syrya [Overview of officinal and promising medicinal plant materials]. Fitoterapiya v Ukraine – Phytotherapy in Ukraine, 1, 10-11 [in Russian].
Maltseva, N.M., Haievskyi, A.P., & Derevianko, K.Yu. (2011). Vplyv biolohichno aktyvnykh rechovyn ta yikh komposytsii na vmiat fotosyntetychnykh pihmentiv u lystkakh ozymoi pshenytsi v umovakh defitsytu fosforu [Influence of biologically active substances and their compositions on the content of photosynthetic pigments in leaves of winter wheat under conditions of phosphorus deficiency]. Fiziologiya i biokhimiya kult. rasteniy – Physiology and Biochemistry of Cultivated Plants, 43 (5), 4403-411.
Simonova, M. (2010). Karotynoidy: budova, vlastyvosti ta biolohichna diia. Biolohichni studii [Carotenoids: structure, properties and biological effects. Biological Studios]. Studia Biologica, 4 (2), 159-170 [in Ukrainian].
Lyakhovych, V.V., Vavilin, V.A., & Zenkov, N.K. (2006). Aktivnaya zashchita pri okislitelnom sresse. Antioksidant-responsivnyy element [Active protection with oxidative stress. Antioxidant-resisting element]. Biokhimiya – Biochemistry, 71 (9), 1183-1197 [in Russian].
Jyonouchi, H., Sun, S., Iijima, K., & Myron D. Gross (2000). Antitumor activity of astaxanthin and its mode of action. Nutr. Cancer, 36 (1), 59-65.
Wang, J.Y., Wen, L.L., & Huang, Y.N., Chen, Y.T., & Ku, M.C. (2006). Dual effects of antioxidants in neurodegeneration: direct neuroprotection against oxidative stress and indirect protection via suppression of glia-mediated inflammation. Curr. Pharm. Des., 12 (27), 3521-3533.
Krinsky, N. I., & Johnson, E.J. (2005). Carotenoid actions and their relation to health and disease. Mol. Aspects Med., 26 (6), 459-516.
Voutilainen, S., Nurmi, T., Mursu, J., & Rissanen, T.H. (2006). Carotenoids and cardiovascular health. Am. J. Clin. Nutr., 83 (6), 1265-1271.
Olson, J.A. (1986). Carotenoids, vitamin A and cancer. J. Nutr., 116 (6), 1127-1130.
Chwiej, M. (1983). Studia i opracowanie metody oraz zalozen technologiczno-projecto-wych do wydobycia likopenu z odpadow pomidorowych. Warszawa.
Counsen, J.N., & Kneustubb, C.J. (1983). Some of natures’s food colours-carotenoids and ribofla-vins. Food Flavour., Clnged. Process. and Packag., 5 (8), 18-19.
Merzlova, H.V., & Merzlova, H.V. (2014). Vmist khlorofilu u biomasi spiruliny za dii riznykh doz mikroelementiv u pozhyvnomu seredovyshchi [The content of chlorophyll in biomass spirulina for the effects of various doses of trace elements in the nutrient medium]. Biolohiia tvaryn – Animals’ Biology, 16 (2), 71-76 [in Ukrainian].
Pihmenty. Khimichna pryroda [Pigments Chemical nature]. [Electronic resource] Korotkyi dovidnyk biolohichno aktyvnykh rechovyn: Pihmenty – Short guide to biologically active substances: Pigments. Retrieved from: http://beclan.org/practical/rechovin_pgmenti.htm [in Ukrainian].
Ternynko, I.I., & Burtseva, O.V. (2010). Vyvchennia lipofilnykh kompleksiv travy ta zerna vivsa posivnoho (Avena sativa L.) [Study of lipophilic complexes of herb and grains of oat seeding (Avena sativa L.)]. Farmatsevtychnyi zhurnal – Pharmaceutical Journal, 1, 89-95 [in Ukrainian].
Marchyshyn, S.M., & Stoiko, L.I. (2015). Pihmentnyi sklad lipofilnoi fraktsii travy zolototysiachnyka zvychainoho [The pigment composition of the lipophilic fraction of the herb of the common centuary]. Ukrainskyi biofarmatsevtychnyi zhurnal – Ukrainian Biopharmaceutical Journal, 1, 65-68 [in Ukrainian].
