GUMORAL CHANGES IN BLOOD OF RESEARCHED ANIMALS IN THE GYPEREGIC TYPE OF INFLAMMATORY REACTION UNDER CONDITIONS OF EXPERIMENTAL GASTRODUODENITY

Abstract

Introduction. To date, it is proved that the leading factor in the development and progression of various diseases is not the source that caused the pathological condition, but violations caused by altered, strengthened or vice versa, weakened immune processes in the body.

The aim of the study – to estimate humoral changes in the blood of animals with hyperergic type of inflammatory reaction under experimental gastroduodenitis.

Research Methods. Experiments were performed on rats weighing 180–200 g. In all animals, acute gastroduodenitis was modeled. Hyperergic type of inflammatory response was modeled by intramuscular administration of pyrogenal in a physiological solution. Normoergic type of inflammatory response – without the additional administration of any substances. Animals were divided into 3 groups (6 animals per group) for the experiment. Determination of Ig A, M, G in blood serum was carried out using the Chernushenko E. F. method. The results were processed in the department of system statistical research in the software package Statsoft STATISTIKA.

Results and Discussion. In animals with hyperergic type of inflammatory response, the content of circulating immune complexes in comparison with control was higher at the 7^th day of the study – by 84 % (p<0.05), by the 10^th day – by 89.4 % (p<0.05). Comparing the indices of the same group of animals with normoergic type of inflammatory response found that the content of the circulating immune complexes was higher at the 7^th day of the study – by 7.3 % (p<0.05), at the 10^th day – by 6.3 % (p<0.05). In rats with hyperergic type of inflammatory response, the level of immunoglobulins of all three classes was higher at the 7^th day of the study – by 76 % (p <0.05), at the 10^th day – by 78.2 % (p<0.05); Ig M level was higher at the 7^th day of the study – by 24.4 % (p<0.05), for the 10^th day – by 23.4 % (p<0.05); IgG level was higher at the 7^th day of the study – by 69 % (p <0.05), for the 10^th day – by 70.1 % (p<0.05). The levels of Ig A, M, G, and the content of circulating immune complexes for the 10th day of serum blood test in all animal groups were elevated.

Conclusion. The most significant changes in immunological parameters were found in animals with hyperergic type of inflammatory response, and changes in humoral immunity were accompanied by an increase level in immunoglobulins A, M, G classes and circulating immune complexes for 10 ^th days.