PECULIARITIES OF OXIDATIVE PROCESSES AT EXPERIMENTAL NONPENETRATING MECHANICAL CORNEAL TRAUMA INJURY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i3.9585Keywords:
corneal injury, oxidative stress, dynamics, experimentAbstract
Introduction. Disorganization of peroxidation processes plays an important role in the pathogenesis of a mechanical nonpenetrating corneal injury [11], but these interrelationships were not been studied sufficiently, which led us to study the proper direction of this.
The aim of the study – to investigate the changes in the intensity of peroxide oxidation of lipids, proteins, and the content of nitrogen monooxide metabolites in rabbit blood under conditions of mechanical nonpenetrating corneal injury in dynamics.
Materials and Methods. Experimental studies were carried out on mature rabbits of the Chinchilla breed. For the study of the features of free radical oxidation at experimental mechanical nonpenetrating eye injury spectrophotometrically, the concentration of hydroperoxides, the content of TBA-reactants, degree of oxidative modification of proteins, level of metabolites of nitrogen monoxidewere determined.
Results and Discussion.It was established the increase in the content of proteins and lipid peroxidation products in rabbits during the mechanical nonpenetrating corneal injury. The results of our study indicate a wave-like dynamics of changes in end products of nitrogen monoxide in serum of rabbits with mechanical non-penetrating corneal injury.
Conclusions. Under the condition of an experimental non-penetrating corneal injury, there is a significant increase of lipid peroxidation (increased levels of hydroperoxides and TBA-active products) and oxidation modification of proteins (aldehyde- and ketone derivatives of dinitrophenylhydrazones of neutral and basic nature), compare to control, with minimum values by 7 days and maximum – by 14 days (p<0.05).Experimental non-penetrating corneal injury is accompanied by a decrease in the level of end products of nitrogen monoxide by 3–7 days on average by 26.0%, followed by an increase of 10 and 14 days, respectively, by 8.0% and 24.9%, relative to the control group ( p<0.01).
References
Patel Daksha (2015). Eye injuries: improving our practice. Cоmmunity Eye Health Journal, 28 (91), 41-42.
Squissato, V., Brown, G., & Baxter, S. (2015). Get out of a scrape! An approach to corneal foreign bodies and abrasions for the primary care physician. Occup. Med. Health Aff., 3, 202. doi:10.4172/2329-6879.1000202
Bhogal, G., Tomlins, P. J., & Murray, P. I. (2007). Penetrating ocular injuries in the home. J. Public Health, 29, 72-74.
Chudnyavtseva, N.A. (2010). Vosstanovitelnoye lecheniye bolnykh s travmami glaza, oslozhnennymi iridodializom [Restorative treatment of patients with eye injuries complicated by iridodialysis]. Oftalmologicheskiy zhurnal – Ophthalmological Journal, 1, 102-105 [in Russian].
Xiang, H., Stallones, L., Chen, G., & Smith, G.A. (2005). Work related eye injuries treated in hospital emergency departments in the US. Am. J. Ind. Med., 48, 57-62.
Chen, S.Y., Fong, P.C., Lin, S.F., Chang, C.H., & Chan, C.C. (2009). A case-crossover study on transient risk factors of work-related eye injuries. Occup. Environ. Med., 66, 517-522.
Harris, P.M. (2006). Nonfatal occupational injuries involving the eye, 2004. US Bureau of Labor Statistics, 1-7.
Ho, C.K., Yen, Y.L., Chang, C.H., Chiang, H.C., Shen, Y.Y., et al. (2007). Epidemiologic study on work-related eye injuries in Kaohsiung, Taiwan. Kaohsiung J. Med. Sci., 23, 463-469.
Reheda, M.S., & Baida, M.L. (2013). Rol porushen perekysnoho okysnennia lipidiv ta aktyvnosti fermentiv antyoksydantnoho zakhystu v trakhei morskykh svynok u patohenezi eksperymentalnoho alerhichnoho alveolitu ta yikh korektsiia tiotriazolinom [Role of violations of lipid peroxidation and activity of antioxidant enzymes in the guinea pig trachea in the pathogenesis of experimental allergic alveolitis and their correction by thiotriazolinum]. Eksperymentalna ta klinichna fiziolohiia ta biokhimiia – Experimental and Clinical Physiology and Biochemistry, 1, 47-51 [in Ukrainian].
Beskyi, V.O., Hryshchuk, L.A., & Marushchak, M.I. (2014). Pokaznyky vilnoradykalnoho okysnennia krovi ta bronkhoalveoliarnoho lavazhu pry syndromi hostroho ushkodzhennia lehen [Indicators of free radical oxidation of blood and bronchoalveolar lavage with acute lung injury syndrome]. Suchasni medychni tekhnolohii – Modern Medical Technology, 2, 48-54 [in Ukrainian].
Turchyn, M.V., & Klishch, I.M. (2014). Osoblyvosti protsesiv vilnoradykalnoho okysnennia krovi ta vodianystoi volohy za umov eksperymentalnoi mekhanichnoi nepronykaiuchoi travmy rohivky [Features of processes of free radical oxidation of blood and aqueous humor under the conditions of experimental mechanical non-penetrating corneal trauma]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Researches, 4, 103-107 [in Ukrainian].
Vlizlo, V.V., Fedoruk, R.S., & Ratych, I.B. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni [Laboratory methods of research in biology, livestock and veterinary medicine]. Lviv: SPOLOM [in Ukrainian].
Fedorova, T.N., Korshunova, T.S., & Larskiy, E.G. (1983). Reaktsii s tiobarbiturovoy kislotoy dlya opredeleniya malonovogo dialdegida krovi metodom fluorimetrii [Reactions with thiobarbituric acid for the determination of malonic dialdehyde by fluorometry]. Laboratornoe delo – Laboratory Work, 3, 25-28 [in Russian].
Meshchyshen, I.F. (1998). Metod vyznachennia okysniuvalnoi modyfikatsii bilkiv plazmy (syrovatky) krovi [Method of determination of oxidative modification of plasma proteins (serum)]. Bukovynskyi medychnyi visnyk – Bukovyna Medical Bulletin, 2, 1, 156-158 [in Ukrainian].
Emchenko, N.L., Tsyganenko, O.I., & Kovalevskaya, T.V. (1994). Universalnyiy metod opredeleniya nitratov v biosredakh organizma [A universal method for the determination of nitrates in the body's biomass]. Klinicheskaya laboratornaya diagnostika – Clinical Laboratory Diagnostics, 6, 19-20 [in Russian].
Oshida, E., Matsumoto, E., & Arai, К. (2010). Free radicals in the aqueous humor of patients with glaucoma. Clinical Ophthalmology, 4, 653-660.
Vinson, J.A. (2006). Oxidative stress in cataracts. Pathophysiology, 13, 151-162.
Dikal, M.V. (2013). Okysniuvalna modyfikatsiia bilkiv u pechintsi shchuriv za umov uvedennia 2,4-dynitrofenolu [Oxidation modification of proteins in the liver of rats under the conditions of 2,4-dinitrophenol]. Klinichna ta eksperymentalna patolohiia – Clinical and Experimental Pathology, XII, 2(44), 71-73 [in Ukrainian].
