INFLUENCE OF L-ARGININ DRUGS ON THE CHANGE OF LIPID PEROXIDATION AND ANTIOXIDANT SYSTEMS IN BLOOD UNDER CONDITIONS OF COMBINATION PATHOLOGY – IMMOBILIZATIONAL STRESS AND ADRENALIN DAMAGE OF MYOCARDIUM
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i3.9577Keywords:
peroxide lipid oxidation and antioxidant system, immobilization stress, adrenal damage to the myocardium, L-arginineAbstract
Introduction. The activation of adrenergic and hypothalamic-pituitary-adrenal systems leads to the Selye stress triad, the first component of which is argenal gland hypertrophy and increased synthesis of glucocorticoids and catecholamines. Arginine is a necessary component for the synthesis of proteins and number of biologically important molecules, such as ornithine, proline, polyamines, creatine and agmatine. However, the main role of arginine in human body – to be a substrate nitrogen oxide synthesis.
The aimof the study – to learn the effect of L-arginine on changes in the processes of lipid peroxidation and antioxidant system in the blood of white rats under the influence of acute immobilization stress and adrenaline.
Research Methods. The experiment was carried out on white male rats of the Vistar line weighing 180–200 g, which were divided into 3 groups (two experimental and one control, 10 animals in each).
Results and Discussion. It was revealed that under conditions of combined pathology of immobilization stress and adrenal damage of myocardium before treatment with L-arginine, blood activity in the antioxidant system of catalase and superoxide dismutase decreased in the 5th day of the experiment. The increase of the products of LP – diene conjugates and malonic aldehyde was established. 5days administration of L-arginine results in increased activity of CT (107.2 %) and SOD (74.5 %) in comparison with a group of animals with a combined pathology in the experiment (IS + AIM) prior to treatment, indicating the antioxidant effect of the drug. The use of L-arginine for 5 days reduced the content of diene conjugates by 80.16 % (p <0.05) and MDA by 42.26 % (p <0.05), indicating a decrease in lipid peroxidation processes.
Сonclusion. Consequently, L-arginine is expedient in the future and perspective to be studied in the experiment and clinic in order to correct metabolic disorders in the conditions of experimental IC and APM and to develop methodological recommendations.
References
Koptev, M.M. (2013). Otsinka morfo-funktsionalnykh zmin perypherychnykh viddiliv leheni pislia hostroho immobilizatsiinoho stresu [Evaluation of morpho-functional changes of peripheral lung organs after acute immobilization stress]. Aktualni problemy suchasnoi medytsyny: Visnyk Ukrainskoi medychoi stomatolohichnoi akademii – Current Problems of Modern Medicine: Journal of the Ukrainian Medical Stomatological Academy, 13, 121-124. [in Ukrainian].
Tymoshenko, A.V., & Diahulenko, D.O., & Koptev, M.M. (2014). Otsinka vplyvu hostroho immobilizatsiinoho stresu na sertse shchura [Assessment of the effect of acute immobilization stress on the heart of a rat]. Aktualni problemy suchasnoi medytsyny: Visnyk Ukrainskoi medychoi stomatolohichnoi akademii – Current Problems of Modern Medicine: Journal of the Ukrainian Medical Stomatological Academy, 14, 229-231 [in Ukrainian].
Shkurupii, O.A. (2015). Eksperymentalne obhruntuvania rozvytky nekardiohennoho nabriaky lehen [Experimental study of the development of non-cardiogenic pulmonary edema in surgical patients with limited motor activity]. Visnyk Problem Biolohii I Medytsyny – Bulletin of Biology and Medicine, 4, 68-70 [in Ukrainian].
Meerson, F.Z., & Pshennikova, M.G. (1988). Adaptatsiya k stressornym situatsiyam i fizicheskim nagruzkam [Adaptation to stressful situations and physical loads]. Moscow: Meditsina [in Russian].
Selye, G. (1980). Stress bez stressa [Stress without distress]. Moscow: Meditsina [in Russian].
Radchenko, O.M. (2004). Adaptatsiini reaktsii, ikh znachennia v klinitsi vnutrishnikh khorob [Adaptation reactions, their significance in the clinic of internal diseases]. Doctor’s thesis.Lviv [in Ukrainian].
Radchenko, O.M. (2004). Adaptatsiini reaktsii v klinitsi vnutrishnikh khorob [Adaptation reactions in the clinic of internal diseases]. Monograph. Lviv: Liha-press [in Ukrainian].
Markova, O.O. (1998). Miokardiodystrofiia i reaktyvnist orhanizmu [Myocardial dystrophy and reactivity of the organism]. Ternopil Ukrmedknyha [in Ukrainian].
Böger, R.H. (2007). The pharmacodynamics of L-arginine]. J. Nutr., 137, 1650-1655.
Chatterjee, A., & Catravas, J.D. (2008). Endothelial nitric oxide (NO) and its pathophysiologic regulation. Vascul. Pharmacol., 49, 134-140.
Babushkina, A.V. (2009). L-arginin z tochki zreniya dokazatelnoy meditsiny [L-arginine in terms of evidence-based medicine]. Ukrainskyi medychnyi chasopys – Ukrainian Medical Chronicle, 6, 43-48 [in Ukrainian].
Korolyuk, M.A., Ivanova, L.I., Mayorova, I.G., & Tokarev, V.E. (1998). Metod opredeleniya aktivnosti katalazy [Method of determination of the activity of catalase]. Laboratornoye delo – Laboratory Business, 1, 16-19 [in Russian].
Gurevich, V.S., Kontorshchikov, K.N., & Shatilina, L.V. (1990). Sravnitelnyy analiz dvukh metodov opredeleniya aktivnosti SOD [Comparative analysis of the method-determination of the activity of the SOD]. Laboratornoye delo – Laboratory Business, 4, 44-47 [in Russian].
Kamyshnikova, V.S. (2000). Spravochnik po kliniko-biokhimicheskoy laboratornoy diagnostike [Directory for clinical and biochemical laboratory diagnostics. In two volumes]. Minsk: “Belarus” [in Russian].
Timirbulatov, R.A. (1988). Metod povysheniya intensivnosti SRO lipidsoderzhashchykh komponentov krovi i ego diagnosticheskoe znachenie [Method of increasing the intensity of SRO lipid – containing blood components and its diagnostic test]. Laboratornoye delo – Laboratory Business, 4, 209-211 [in Russian].
Gorizontov, P.D., Belousova, O.I., & Fetodov, M.I. (1983). Stress i sistema krovi [Stress and blood system]. Moscow: Meditsina [in Russian].
Tkach, Ye.I. & Storozhuk, V.P. (2009). Zahalna teoriia statystyky [The general theory of statistics]. Kyiv: Tsentr uchbovoi literatury [in Ukrainian].
