ACTIVITY OF BIOENERGY PROCESSES IN THE ORGANISM OF RATS IMMEDIATELY AFFECTED BY CARBOPHOS AND TETRACHLOROMETHANE AND AFTER CORRECTION BY MEXIDOL
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i3.9575Keywords:
carbophos, carbon tetrachloride, mexidol, succinate dehydrogenase, cytochrome oxidase, glucose, glycogenAbstract
Introduction. Getting into the human body and animals, organophosphorous substances cause the activation of free radical processes, suppress protective and compensatory forces, inhibit the enzymes involved in the energy supply of cells. Tetrachloromethane, a xenobiotic used in industry, agriculture and everyday life, has a toxic effect on the liver. In real life, there is often a combined effect of several toxic factors, which, depending on the conditions, can disrupt the balance of oxidative processes in the body and lead to serious illnesses.
The aim of the study– to learn the intensity of energy supply processes in the body of rats under the conditions of simultaneous destruction of their organophosphorus compounds and carbon tetrachloride, as well as the effective of using the antioxidant mexidol under the given conditions.
Research Methods. The studies were carried out on white male rats, which daily for 30 days were administered carbophos (20 mg/kg) and tetrachloromethane twice – every other day (1 ml/kg). The animals were excised from the experiment under thiopental anesthesia on the 10th and 30th days of carbophosic poisoning and on the 4th and 7th days after the defeat with carbon tetrachloride. In the liver and heart of animals, the activity of succinate dehydrogenase, cytochrome oxidase and glycogen content was determined, and the glucose content in blood serum.
Results and Discussion. During the entire experiment, there was a progressive decrease in succinate dehydrogenase and cytochrome oxidase activity in the liver and myocardium of affected animals, which were the lowest by the end of the study. The content of glucose in the blood serum after the defeat by toxicants was maximized at the end of the experiment. In the same period, the maximum decrease in glycogen content in the liver and myocardium of affected animals was observed. The use of mexidol led to an increase in the activity of mitochondrial enzymes, and also normalized the level of glucose in blood serum and glycogen in the liver and myocardium of affected rats, which confirms its antihypoxic properties.
Conclusions. The combined defeat of rats with tetrachloromethane and carbophos leads to significant disturbances in the energy supply of the organs of the rats. The effective of mexidol as an antihypoxant has been proved.
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