CONTENT OF HYDROXYCINNAMIC ACIDS IN HERB AND RHIZOMES AND ROOTS OF SAXIFRAGE PIMPINELLA (PIMPINELLA SAXIFRAGA L.)
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i3.9571Keywords:
saxifragepimpinella, hydroxycinnamic acids, chlorogenicacid, spectrophotometric method, high performance liquid chromatographyAbstract
Introduction.saxifragepimpinella (Pimpinella saxifraga L.) from the family of celery (Apiaceae) has long been used in folk medicine as expectorant, diuretic, anti-inflammatory, sedative, and pain medication. It is recommended for catarrhal airways, tracheitis, bronchitis, pneumonia, laryngitis, asthma, pertussis, edema, salt diathesis, liver disease, intestinal atony, chronic gastritis with low acidity, gout, swelling and flatulence. With therapeutic purposes, mainly, rhizomes and plant roots are used. There are about 200 species of the genus pimpinella(Pimpinella L.), common in Asia, Europe and Africa, there are 5 species in Ukraine; the most common is saxifrage pimpinella.
The aim of the study– to establish the presence and determine the quantitative content of hydroxycinnamic acids in the herb and subterraneous organs of the saxifrage pimpinella.
Research Methods. To detect hydroxycinnamic acidswe used alcohol-water extractor. The reaction with 1 % solution of ferrum (III) chloride (the appearance of green and gray color) indicated the presence of compounds of phenolic nature in the investigated extraction. The quantitative determination of hydroxycinnamic acids in the investigated objectsisbasedonthespectrophotometricmethod. Qualitative composition and quantitative content of individual hydroxycinnamic acids in the herb and subterraneous organs of saxifrage pimpinella were investigated by high performance liquid chromatography (HPLC) on Agilent 1200 3 D LC System Technologies chromatograph (USA).
Results and Discussion. The quantitative content of the sum of hydroxycinnamic acids in the herb of the saxifrage pimpinella was (4.68±0.05)%, in the subterraneous organs – (1.52±0.03)%. The HPLC method in the investigated objects of the saxifrage pimpinella we revealed, identified and established the quantitative content of acids of chlorogenic and rosemary. Chlorogenic acid, the content of which was 3.13% and 0.11% respectively, is mainly the dominant inherb and subterraneous organs of the saxifrage pimpinella. Saxifrage pimpinella herb contains 0.34% of rosemary acid.
Conclusions. The presence and determination of the quantitative content of hydroxycinnamic acids in grass and subterraneous organs of the saxifrage pimpinella which was (4.68±0.05)% and (1.52±0.03) %, respectively, was established. The method of HPLC analysis in the investigated raw material of the saxifrage pimpinella established individual hydroxycinnamic acids – chlorogenic and rosemary, and their quantitative content is determined. The dominant in the herb and subterraneous organs of the saxifrage pimpinella is chlorogenic acid, which content was 3.13% and 0.34%, respectively, therefore it can be recommended for the standardization of raw materials.
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