Hematological and cytochemical parameters of peripheral blood of Wistar Hannover rats after exposure to epoxiconazole
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i1.8843Keywords:
epoxiconazole, hematotoxicity, anemia, succinate dehydrogenase, acid phosphatase, naphthol-AS-D-chloroacetateterase, macrophages, "tailed" lymphocytes.Abstract
Introduction. Epoxiconazole is a highly effective triazole fungicide. It is a part of many modern compounds for the control of phytopathogens. Widely used in agriculture in all over the world and in Ukraine, in particular. Despite its hepatotoxic characteristics, this fungicide alters the hematological parameters of blood.
The aim of the study – to investigate the effects of generic triazole fungicide epoxiconazole, 95% on hematological and cytochemical parameters of peripheral blood of Wistar Hannover rats in acute experiment.
Research Methods. 10 healthy males of Wistar Han rats were equally divided into control (0 mg/kg/bw) and experimental groups. Dose 1580 mg/kg/bw of epoxiconazole (1/2 LD50) was administrated once orally by gavage to 5 experimental rats. Peripheral blood was studied at 0 and 1, 3, 7, 14 day after exposure (DAE). RBC, HGB, HCT, erytrocyte indices MCV, MCH, MCHC, WBC and PLT were, hemogram and morphological disturbances of cells were studied,percentage ratio of different types of leukocytes was calculated.The cytochemical status of leukocytes was assessed on the basis of determining the enzymatic activity of specific naphthol-AS-D-chloroacetateterase in neutrophils, succinate dehydrogenase and acid phosphatase in lymphocytes.
Results and Discussion. Erythrocytosis in response to pesticide intoxication with a decrease of mean cellhemoglobin concentration in one erythrocyte; the reduction of hemoglobin concentration in the end of experiment against the background of active polychromasia confirmed the anemic effect of epoxiconazole. Reactive neutrophilia, stimulation of lymphocytopoiesis and monocytopoiesis, appearance of macrophages in the peripheral blood, increased activity of naphthol-AS-D-chloroacetateterase, succinate dehydrogenase and acid phosphatase were considered as compensatory mechanisms. Reverse quantitative changes in the ratio of lymphocytes subpopulations based on the activity of acid phosphatase were noted.
Conclusions. Results of the study of generic fungicide epoxiconazole, 95% hematotoxic activity in the acute experiment on Wistar Hannover male rats showed the development of latent anemia, which was manifested in the late-term study; activation of monocytopoesis; increase of macrophages in the peripheral blood; changes in the immune response of the body and the appearance of atypical normocytes and lymphocytes with nuclear abnormalities.
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