Metabolic processes in the small intestine wall, heart and liver in experimental intake of carrageenan
DOI:
https://doi.org/10.11603/mcch.2410-681X.2017.v0.i3.8201Keywords:
κ-carrageenan, lipid peroxidation, small intestine, heart, liver, experiment.Abstract
Introduction. In the scientific literature there is a large number of publications on the chemical modification of carrageenans, the relationship between the chemical structure and their biological activity, and new opportunities for their usage in medicine. That is whyit is modern to study the properties and the state of organs and systems of the body in case of carrageenan intake.
The aim of the study – to learn the indices of metabolic processes in the wall of the small intestine, myocardium and liver of experimental animals using 1% carrageenan solution.
Research Methods. The study was carried out on 24 white non-linear male rats. The animals of the experimental group were provided with free access to a 1.0% of carrageenan solution for 1 month. In the selected samples of the small intestine, heart and liver was evaluated the intensity of the processes of lipid peroxidation by the content of hydroperoxides and TBA-active products.
Results and Discussion. It was found that the intake of a 1% solution of κ-carrageenan led to activation of lipid peroxidation both in the wall of the small intestine and in the tissues of the liver and myocardium, however, their intensity was different. The lipoperoxidation activity was highest in the small intestine wall, while the hydroperoxides and TBA-active products were less in the myocardial tissues. So, the oral usage of carrageenan in the form of a 1% water solution has a direct and indirect damaging effect on the free radical processes in the body.
Conclusions. Oral administration of a 1% carrageenan solution in experimental animals leads to statistically significant activation of free radical oxidation processes in the small intestine wall, heart and liver tissues, characterized by an increase in the content of both primary and secondary products of lipoperoxidation (p<0.05).
References
Martynov, S.V., & Marenkova, T.I. (2015). Zastosuvannia kappa-karahinanu u skladi kharchovykh produktiv [The use of kappa-carrageenan in food products]. Materialy naukovoi konferentsii studentiv Sumskoho NAU – Materials of the Conference of Students of Sumy NAU, 182 [in Ukrainian].
Keppeler, S., Ellis, A.,& Jacquier, J.C. (2009). Cross-linked carrageenan beads for controlled release delivery systems. Carbohydr Polym., 78, 973-977.
Yermak, I.M.,& Khotimchenko, Y.S. (2003). Chemical properties, biological activities and applications of carrageenan from red algae. Recent Advances in Marine Biotechnology, 23, 207-255.
Podobii, O., & Ladonko, M. (2016). Vplyv karahinanu na fizyko–khimichni vlastyvosti zubnykh past [Effect of carrageenan on the physical and chemical properties of toothpastes]. II Vseukrayinska naukovo-praktychna konferentsiia “Aktualni problemy khimii ta khimichnoi tekhnolohii” – II Ukrainian Scientific and Practical Conference "Actual Problems of Chemistry and Chemical Technology",Kyiv [in Ukrainian].
Sokolova, E.V., Barabanova, A.O., Bogdanovich, R.N., Khomenko, V.A., Soloveva, T.F., & Yermak, I.M. (2011). In vitro antioxidant properties of red algal polysaccharides. Biomedicine and Preventive Nutrition, 1, 3. 161-167.
Yermak, М.І., Byankina (Barabanova), А.О., & Sokolova E.V. (2014). Structural peculiarities and biological activity of carrageenans – sulphated polysaccharides from red algae collected from the Russian Pacifi c coast. Bulletin of the Far Eastern Branch of the Russian Academy of Sciences, 1, 80-92 [in Russian].
Kharchenko, Ye.A., Sklyaruk, D.O., &Tkachenko, A.S. (2017). Vliyanie peroralnogo upotrebleniya pishchevoy dobavki E407A (κ- karraginan) na tonkiy kishechnik[Effect of oral use of food supplement E407A (κ-carrageenan) on the small intestine]. “KhIST” Vseukraїnskyi medychnyi zhurnal molodykh vchenykh – "HIST" All-Ukrainian Medical Journal of Young Scientists, 19, 58[in Ukrainian].
Tkachenko,A.S., Gopkalov, V.G., & Orlova, M.A. (2014). Sistema generatsii oksida azota pri eksperimentalnom gastroenterokolite [System of generation of nitrogen oxide with experimental gastroenterocolitis].Bukovynskyi medychnyi visnyk – Bukovyna Medical Journal, 18, 2 (70), 109-112 [in Ukrainian].
Tobacman, J.K. (2001). Review of harmful gastrointestinal effects of carrageenan in animal experiments. Environmental Health Perspectives, 109, 10, 983-994.
Moyana, T.N., & Lalonde, J.M. (1990). Carrageenan-induced intestinal injury in the rat – a model for inflammatory bowel disease. Ann. Clin. Lab. Sci., 20, 6, 420-426.
Hubina-Vakulyk, H.I., Kolousova, N.H., Ivanenko, Т.О., Horbach, T.V., Korobchanskyi, V.O. Sposib modeliuvannia khronichnoho hastroenterokolitu [Method of modeling chronic gastroenterocolitis]. Patent Ukraina, № a201014510, 2012.
Nesterova, L.A., Smurova, E.A., & Manukhin, B.N. (1995). Characterization of specific binding blocker [+ H]-quinuclidinylbenzilate M-cholinergic receptors in rat brain cortex membranes. Reports of the Academy of Sciences, 343 (2), 268-271 [In Russian].
Lowry, O.H. (1951). Protein measurement with the Folin phenol reagent. Journal of Biоlogical Chemistry,193(1), 404-415.
Oleksyuk, N.P.,& Yanovych, V.G. (2010). The activity of pro- and antioxidant systems in the liver of freshwater fish in different seasons. The Ukrainian Biochemical Journal, 82 (3), 41-48.
Timirbulatov, R.R.,& Selezneva, E.I. (1981). Method for increasing the intensity of free radical oxidation of lipid-blood components and its diagnostic value. Laboratory Work, 4, 209-211.
Glanc, S. (1999). Mediko-biologicheskaya statistika[Medical and Biological Statistics]. Moscow: Praktika [in Russian].
Rebrova, O.Yu. (2002). Statisticheskiyanalizmeditsinskikhdannykh. PrimeneniepaketaprikladnykhprogrammSTATISTICA [Statistical analysis of medical data. Application of software package STATISTICA]. Moscow: MediaSfera [in Russian].
Moyana, Terence, &Lalonde, J.M.Antoine. (2016). Carrageenan-induced Intestinal Injury: Possible Role of Oxygen Free Radicals. Annals of Clinical and Laboratory Science, 21, 4, 258-263.
Tkachenko, A.S., Gopkalov, V.G., & Martyinova, S.N. (2015). Sposob korrektsii khronicheskogo karraginan-indutsirovannogo gastroenterokolita vitaminami-antioksidantami[The method of correction of chronic carrageenan-induced gastroenterocolitis with antioxidant vitamins]. Visnyk problem biolohii i medytsyny –Journal of Biology and Medicine, 3, 1(122), 315-317 [in Ukrainian].
Nesterov, Yu.V., Chumakova, A.S., & Turchenko, N.V. (2010). Vliyanie stress-indutsirovannykh vozdeystviy raznoy modalnosti i antioksidanta na svobodnoradikalnyie protsessy v legkih i pecheni belykh krys [The influence of stress-induced effects of different modalities and antioxidant on free-radical processes in the lungs and liver of white rats]. Estestvennyyenauki – NaturalSciences, 3,122-126 [in Russian].
Hidirova, L.D. (2010). Izmenenie balansa mezhdu aktivnostyu perekisnogo okisleniya lipidov, antioksidantnoy zaschitoy i soderzhaniem zheleza u krys pri eksperimentalnom infarkte miokarda [Change in the balance between lipid peroxidation activity, antioxidant protection and iron content in rats with experimental myocardial infarction]. Ratsionalnaya farmakoterapiya v kardiologii – Rational Pharmacotherapy in Cardiology, 3, 122-126[in Russian].
Chumakova, A.S., Teplyiy, D.L., & Nesterova, Yu.V. (2009). Izmenenie svobodnoradikalnykh protsessov v razlichnykh organakh krys raznogo vozrasta pri ostrom stresse [Changes in free radical processes in various organs of rats of different ages in acute stress]. Biologicheskie issledovaniya –Biological Research, 4, 34-37 [in Russian].
