Analytical support of hygienic control of оxathiapiprolin residual amounts in environmental objects and potatoes
DOI:
https://doi.org/10.11603/mcch.2410-681X.2017.v0.i3.8189Keywords:
оxathiapiprolin, liquid chromatography, air, water, soil, potatoes.Abstract
Introduction. Providing of analytical control over the application of pesticides in environmental objects and agricultural products – is a mandatory component of preregistration studies of new compounds. The most common method for the control of pesticides application in theUkraine is high performance liquid chromatography.
The aim of the study – to develop analytical methods for оxathiapiprolin determination in the air, water, soil and potatoes for hygiene monitoring of compliance with hygienic standards in the environment and agricultural raw materials.
Research methods. Оxathiapiprolin – is a compound from new рiperidinyl-thiazole-isoxazolines chemical class. For investigation the HPLC method was used.
Results and Discussion. Optimal conditions of oxathiapiprolin chromatography were established: mobile phase – acetonitrile mixture + bidistilled water (75+25), UV detector wavelength – 260 nm, thermostat column temperature – 30 °С. Retention time – 4.6±0.1 minutes. Linear detection range is 0.5–10.0 mg/ml. Dependence of calibration area (S) peaks of the substance on its concentration (r) described by linear regression equation: Soxathiapiprolin = 272.4 + 36315.7´r.
The best results are obtained when concentrating the air on a filter paper “blue ribbon” followed by extraction with acetone. When choosing extractants to оxathiapiprolin determination in water, the best result obtained with ethylacetate; soil – with acetone, potatoes – with a mixture of acetonitrile + 0.5 % aqueous phosphoric acid, followed by purification of extracts by adsorption.
Conclusions. Thus, developed analytical methods meet modern requirements, are selective and allow to control oxathiapiprolin content in water at detection limit level – 0.002 mg/dm3, air of working zone – 0.2 mg/m3, atmosphere air – 0.003 mg/m3, soil – 0.03 mg/kg, potato – 0.003 mg/kg.
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