Protein oxidative modification in white rats of different ages under acute poisoning by amanita phalloides toxins
DOI:
https://doi.org/10.11603/mcch.2410-681X.2017.v0.i1.7680Keywords:
Amanita phalloides, poisoning, protein oxidative modification, age-related features of poisoning.Abstract
Introduction. In recent years, scientific articles of domestic and foreign scientists obtained numerical data on the important role of oxidative modification of proteins in the development of many diseases. It is known that entering the organism of toxic compounds, including toxins of Amanita phalloides, sharply oppress antioxidant system increases the contents of peroxidation products lipids and proteins in liver, kidneys.
The aim of the study – to examine the dynamics of oxidative modification of proteins in rats of different ages affected by poison Amanita phalloides toxins in different periods of the experiment.
Methods of the research. In all experimental groups poisoning was modeled by single intraperitoneal administration Amanita phalloides extract obtained by the method of H. Wieland (85 mg / kg body weight). Euthanasia was performed under sodium thiopental anesthesia at 6, 24 and 72 hours after poisoning. Oxidative modification of proteins in blood serum was investigated by Meschyshen method.
Results and Discussion. Protein oxidative modification indices in all age groups rats on the model of toxic injury by Amanita phalloides toxins were investigated. It was found out that the progression of the pathological process in the liver and the depletion of the compensatory capacity in animals with acute poisoning by Amanita phalloides leads to the activation of free radical oxidation of proteins and cause the increasing of protein oxidative modification indices. The most pronounced changes occur in 6- and 18- month rats in 24 hours after intoxication.
Conclusions. It was defined, that the progression of the pathological process in the liver and the depletion of the compensatory capacity as a result of poisoning by toxins of Amanita phalloides cause the increasing of protein oxidative modification indices. It can be considered as one of the earliest markers of damage to organs and tissues including hepatocytes and reliable early predictor of the development and pathology related oxidative stress.
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