DYNAMICS OF INDICATORS OF ENDOGENOUS INTOXICATION, ANTIOXIDANT AND IMMUNE SYSTEM IN MODELED TROPHIC ULCER IN RATS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2026.i1.16021Keywords:
trophic ulcer; PRP therapy; antioxidant system; endogenous intoxication.Abstract
Introduction. Trophic ulcers are a serious complication of various diseases, such as chronic venous insufficiency, diabetes mellitus and occlusive vascular diseases. Traditional treatment methods often do not provide a complete cure, therefore it is necessary to explore effective regenerative approaches. The aim of the study. To experimentally trace the dynamics of the development of endogenous intoxication, antioxidant and immune systems in the body of white laboratory nonlinear rats in trophic tissue ulcers. Materials and Methods. The experimental study was conducted on sexually mature white male rats weighing 200–220 g, randomly selected from the vivarium of the I. Ya. Horbachevsky National Medical University of the Ministry of Health of Ukraine. Experimental model of trophic ulcer. All experimental procedures were performed in accordance with the ethical principles approved by the First National Congress on Bioethics and in accordance with the requirements of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. Statistical processing of the obtained data was carried out using the Statistica 10.0 software (StatSoft Inc., USA). Results and Discussion. The results obtained indicate that the development of the simulated trophic ulcer is accompanied by a significant increase in the synthesis of ceruloplasmin, which reflects the activation of the AOS system in response to the increase in the intensity of free radical processes. Complete normalization of MSM(254) on the 28th day is an effective elimination of low-molecular toxins, while a slight hyperconcentration of MSM(280) indicates the presence of residual intoxication phenomena. Conclusions. The development of experimental trophic ulcers is accompanied by activation of lipid peroxidation processes, an increase in the level of DC, and an increase in the level of malondialdehyde. Only by the 28th day is a tendency to normalization observed, which may be a consequence of reparative processes and stabilization of membrane structures.
References
Al-Kun, O., & Boudina, S. (2013). Cardiac dysfunction and oxidative stress in the metabolic syndrome: An update on antioxidant therapies. Current Pharmaceutical Design, 19 (27), 4806–4817. DOI: https://doi.org/10.2174/1381612811319270003
Kábelová, A., Malínská, H., Marková, I., Oliyarnyk, O., Chylíková, B., & Šeda, O. (2021). Ellagic acid affects metabolic and transcriptomic profiles and attenuates features of metabolic syndrome in adult male rats. Nutrients, 13 (3), 804. DOI: https://doi.org/10.3390/ nu13030804
Dzubanovsky, I. Y., Pidruchna, S. R., Melnyk, N. A., Andreychyn, S. M., Vervega, B. B., & Nychyk, N. A. (2020). Dynamics of cytokine profile indicators changes in animals with acute generalized peritonitis on the background of diabetes mellitus. Journal of Medicine and Life, 13 (3), 404–409.
Ofosu, F. K., Mensah, D. F., Daliri, E. B. M., & Oh, D. H. (2021). Exploring molecular insights of cereal peptidic antioxidants in metabolic syndrome prevention. Antioxidants, 10 (4), 518. DOI: https://doi.org/10.3390/ antiox10040518
Pidruchna, S. R., Benedyk, V. V., Piatnochka, V. I., Melnyk, N. A., & Zakharchuk, U. M. (2021). Changes of pro- and antioxidant indicators in experimental animals under acute small bowel obstruction. Journal of Medicine and Life, 14 (1), 32–36. DOI: https://doi.org/10.25122/jml-2020-0066
Dzhyvak, V. H., & Klishch, I. M. (2020). The effectiveness of platelet-rich plasma in inducing muscle tissue healing in an experimental study. Hospital Surgery (Journal named after L. Ya. Kovalchuk), (3), 36–43. DOI: https://doi.org/10.11603/2414-4533.2020.3.11461 (in Ukrainian)
Dzhyvak, V., Klishch, I., Datsko, T., & Khlibo- vska, O. (2020). Influence of PRP on morphological changes in muscle in the early period after traumatic muscle injury in the experiment. Journal of Education, Health and Sport, 10 (6), 171–178. DOI: http://dx.doi.org/ 10.12775/JEHS.2020.10.06.019
Castrejón-Téllez, V., Villegas-Romero, M., Rubio- Ruiz, M. E., et al. (2020). Effect of a resveratrol/quercetin mixture on the reversion of hypertension induced by a short-term exposure to high sucrose levels near weaning and a long-term exposure that leads to metabolic syndrome in rats. International Journal of Molecular Sciences, 21 (6), 2231. DOI: https://doi.org/10.3390/ijms21062231
Pidruchna, S. R., Melnyk, N. A., Mochulska, O., Horishniy, I. M., & Sheremet, M. I. (2019). Dynamics of indicators of cellular immunity in conditions of acute generalized peritonitis in rats. Biointerface Research in Applied Chemistry, 9 (6), 4663–4666. DOI: https://doi. org/10.33263/BRIAC96.663666
Farhangi, M. A. (2020). Dietary total antioxidant capacity significantly interacts with VEGF +405 G/C (rs2010963) gene polymorphisms in terms of cardio- metabolic risk factors in patients with metabolic syndrome. BMC Research Notes, 13, 145. DOI: https://doi.org/10.1186/s13104-020-04993-8
State Institution “V. Ya. Danilevsky Institute of Endocrine Pathology Problems of the National Academy of Medical Sciences of Ukraine”. (2019). Modeling of metabolic syndrome of different genesis in experimental animals: Guidelines.
Andreeva, L. I., Kozhemyakin, L. A., & Kish- kun, A. A. (1988). Modification of the method for determination of lipid peroxidation products using thiobarbituric acid. Laboratornoye Delo, (11), 41–43.
Kolb, V. G., & Kamyshnikov, V. S. (1982). Handbook of Clinical Chemistry. Minsk: Belarus.
Korolyuk, M. A., Ivanova, L. I., & Mayo- rova, I. G. (1988). A method for determining catalase activity. Laboratornoye Delo, (11), 16–18.
Levitsky, A. P., Pochtar, V. M., Makarenko, O. A., & Gridina, L. I. (2006). Antioxidant-prooxidant index of serum in rats with experimental stomatitis and its correction with dental elixirs. Odesa Medical Journal, (1), 22–25.
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Accepted 2026-04-07
Published 2026-04-28
