ASPARTATE AMINOTRANSFERASE ACTIVITY IN BLOOD SERUM IN THE DYNAMICS OF MECHANICAL TRAUMA OF VARIOUS LOCATIONS, COMPLICATED BY ACUTE BLOOD LOSS

Abstract

Introduction. Trauma is one of the leading causes of mortality and morbidity all over the world. Today, it’s thought that the increase in blood serum aspartate aminotransferase (AST) activity is linked to systemic disorders that are different from parenchymal liver diseases. However, the influence of mechanical trauma of various locations and acute blood loss on the dynamics of blood serum AST activity has not been sufficiently studied, which required special research. The Aim of the Study is to determine blood serum AST activity in the dynamics of traumatic brain injury (TBI), blunt abdominal trauma (BAT), and skeletal trauma (ST) complicated by acute blood loss. Research Methods. Experiments were conducted on white mature male Wistar line rats. Under conditions of thiopental sodium anesthesia, rats were modeled with TBI, BAT, and ST, standardized by mortality rate. In some groups of rats, acute blood loss in the amount of 1,5 % of body weight was additionally simulated. The rats were taken out of the experiment after 3, 7, 14, 21, and 28 days of the post-traumatic period. AST activity was determined in blood serum. Results and Discussion. Modeling of isolated TBI, BAT and ST are accompanied by an increase in systemic membranopathy, accompanied by a significant increase in blood serum AST activity during the period of early and late manifestations of traumatic disease compared to the control. Under conditions of BAT, the indicator is significantly higher compared to other study groups after 7, 14, 21, and 28 days of the post-traumatic period. Complication of simulated injuries with acute blood loss in the amount of 1,5 % of body weight compared to the control group causes a statistically significant increase in blood serum AST activity compared to injured rats with the same trauma: after TBI and acute blood loss – at 3, 7, 21, and 28 days of the post-traumatic period, after BAT and acute blood loss – at all time points of the post-traumatic period, after ST and acute blood loss – at 3-21 days. Starting from the 7th day of the experiment, blood serum AST activity is significantly higher in the context of BAT and acute blood loss compared to other study groups. Conclusions. Mechanical trauma of various localization causes a significant increase in blood serum AST activity during the early and late stages of traumatic disease, which is significantly exacerbated by additional acute blood loss and prevails in rats with BAT.