MANIFESTATIONS OF THE ENDOGENOUS INTOXICATION SYNDROME IN RATS UNDER THE INFLUENCE OF CADMIUM CHLORIDE BASED ON THE CONSUMPTION OF DRINKING WATER WITH DIFFERENT PHOSPHATE CONTENT
DOI:
https://doi.org/10.11603/mcch.2410-681X.2024.i3.14918Keywords:
drinking water, rats, heavy metals, endogenous intoxication, phosphates, cadmium, medium mass molecules, erythrocyte intoxication indexAbstract
Introduction. For many years, the pollution of surface water bodies by phosphates and heavy metals has been an urgent problem for Ukraine. The result of their toxic effect on the body of people and animals can be the syndrome of endogenous intoxication.
The aim of the study - to establish the peculiarities of the syndrome of endogenous intoxication in white rats under the influence of cadmium against the background of drinking water with different phosphate content for 30 days.
Research Methods. The study was conducted on 72 purebred white male rats weighing 180-200 g, divided into six groups, which for 30 days consumed: the control group - settled water from the city waterworks, the animals of five experimental groups – water with the addition of sodium monophosphate in doses of 100,0; 10.0; 1.0; 0.1; 0.01 mg/dm3 in terms of elemental phosphorus. On the 25th day of the experiment, each group of rats was divided into two series. The first one continued to drink the same water (n=6), the animals of the second series were injected intragastrically with cadmium chloride at a dose of 1/20 of the maximum permissible concentration for the next five days. The level of endogenous intoxication was assessed by the content of molecules of medium mass (МММ) in blood serum at wavelengths λ=254 and 280 nm and the level of the erythrocyte intoxication index.
Results and Discussion. A negative effect of cadmium chloride was established against the background of drinking water with added phosphates on white rats, as evidenced by a statistically significant increase in the level of endogenous intoxication, as indicated by a significant increase in the content of molecules of medium mass 254 and 280 and an increase in the erythrocyte index of intoxication in blood serum, the most pronounced at a concentration of 100.0 and 10.0 mg/dm3. The level of MМM254 in rats of the 1st group increased by 135 % (p<0.05), in the 2nd group – by 46 %. Indicators of MМM280 in the 1st group increased by 196 % (p<0.001), in the 2nd – by 171 % (p<0.001). The erythrocyte intoxication index in the 1st group exceeded the control by 244 % (p<0.001), in the 2nd – by 188 % (p<0.001).
Conclusions. The obtained results indicate the development of endogenous intoxication under the influence of cadmium chloride against the background of drinking water with different phosphate content
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