DEVELOPMENT OF THE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF PERINDOPRIL IN TABLETS FROM THE POSITION OF THE PRINCIPLES OF “GREEN” CHEMISTRY

Abstract

Introduction. In the scientific literature, spectrophotometric methods for the determination of perindopril in medicines using various reagents are described, however, the presented methods often have limitations, which significantly narrows the scope of application and possibilities of these analytical methods. Therefore, it is promising to expand the base of “green” analytical methods for the determination of perindopril in tablets.

The aim of the work – to develop a simple, express, “green”, inexpensive UV-spectrophotometric method for the quantitative determination of perindopril in tablets.

Research Methods. The object of the study were “Prenelia” tablets 8 mg, batch No. 0068541, manufactured by “Arterium”, pharmacopoeial standard sample of perindopril tert-butylamine (Sigma-Aldrich, ≥98.0 %, HPLC), ethanol (Honeywell Riedel-de Haen™). To perform the experiment, a double-beam scanning spectrophotometer “Shimadzu UV-1800” (Japan) with UV-Probe 2.62 software, quartz cuvettes (1 cm), laboratory electronic scales “RAD wAG AS 200/C”, ultrasonic bath “Sonorex Digitec DT100H”, class A measuring flasks, were used.

Results and Discussion. Previous studies optimized the sample preparation conditions related to the selection of the solvent, wavelength, concentration of solutions, their stability, etc. As a result of the experimental studies on the choice of the optimal solvent, ethanol turned out to be an excellent choice. After that, the corresponding UV spectrum of the ethanol extraction from the studied tablets was obtained, which shows an intensely expressed absorption band with a maximum at a wavelength of 213 nm. LOD was 2.25 μg/mL, LOQ – 6.82 μg/mL, respectively. The regression equation was also presented as y = 0.0179x – 0.5938 and the correlation coefficient (R₂=0.9993) was calculated. Linearity was observed over the entire range of application of the developed quantitative determination method (44.16–70.66 μg/mL), the systematic error was equal to 0.19 %. The evaluation of the “greenness” of the proposed methodology indicates that the analytical technique is excellent according to the principles of “green” chemistry.

Conclusions. As a result of the conducted research, a UV-spectrophotometric method of quantitative determination of perindopril in tablets has been developed, which is express and “green”. The proposed spectrophotometric method for the determination of perindopril in medicines can be used in routine analysis in pharmaceutical quality control laboratories with a limited budget, as it does not require expensive equipment and reagents.