STUDY OF ANTI-EXUDATIVE ACTIVITY OF DRY EXTRACT FROM REISHI MUSHROOMS

Abstract

Introduction. The problem of treating inflammatory process is an important task today, because long-term use of non-steroidal anti-inflammatory drugs can lead to erosion of the mucous membrane of the stomach, duodenum, and negatively affect the kidneys and heart. Therefore, the search and creation of new drugs of natural origin that will exhibit anti-inflammatory and anti-exudative effects is relevant.

The aim of the study – to study the anti-exudative activity of dry extract from reishi mushrooms in an experiment on white rats with carrageenan-induced paw edema of animals.

Research Methods. The experimental work was performed on white outbred male rats weighing 190–230 g. Acute aseptic inflammation was modeled by subplantar injection of 0.1 ml of 1 % carrageenan solution. The dry extract of reishi mushrooms was administered intragastrically at a dose of 100 mg/kg of the animal’s body weight once, one hour before phlogogen administration. The volume of rat paws was measured after 1, 3, 6 and 24 h using a mechanical oncometer. The anti-exudative activity of the studied extract was determined by the degree of reduction of animal paw edema. Diclofenac sodium was chosen as a comparison drug, which was administered at a dose of 8 mg/kg of rat body weight.

Results and Discussion. It was established that the dry extract of reishi mushrooms at a dose of 100 mg/kg of the animal’s body weight exhibits pronounced antiexudative activity in the rat paw carrageenan edema model. The maximum swelling volume of the paw of the animals was recorded 3 hours after the introduction of carrageenan and was 2.8 times greater than that of the control group of animals. The maximum antiexudative activity of the studied extract, which was 43.69 %, was noted 24 hours after the introduction of phlogogen. The comparison drug also showed a pronounced anti-inflammatory effect, with maximum activity at the end of the study – 48.58 %.

Conclusion. It has been proven that the dry extract from reishi mushrooms exhibits pronounced anti-exudative activity in the model of acute inflammation induced by the introduction of carrageenan.