THE ROLE OF GENDER IN DISORDERS OF BILE-FORMING FUNCTION OF THE LIVER UNDER CONDITIONS OF EXPERIMENTAL CRANIOSKELETAL TRAUMA AND THEIR CORRECTION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2024.i2.14772Keywords:
craniocerebral trauma, skeletal trauma, male rats, female rats, liver, bile formation, 2-ethyl-6-methyl-3-hydroxypyridine succinateAbstract
Introduction. The severe trauma is one of the most pressing problems of the third millennium. Among them, the combined cranioskeletal trauma (CST) significantly stands out, which is characterized by a severe course and high mortality. There are gender differences in the course of severe trauma. However, the peculiarities of the course of cranioskeletal trauma in individuals of different sexes continue to be controversial. There is no single opinion on the effectiveness of antioxidants in reducing the systemic manifestations of traumatic disease under these conditions.
The aim of the study – to determine the role of gender in disorders of bile-forming liver function under conditions of experimental CST and the effectiveness of correction with 2-ethyl-6-methyl-3-hydroxypyridine succinate.
Research Methods. The experiments were performed on white Wistar line rats weighing 200-220 g, which included 78 female and 78 male rats, and divided into control and two experimental groups. Under thiopental sodium anesthesia (40 mg·kg- 1), CST was modeled in experimental group 1; in experimental group 2, rats with CST were corrected with a solution of 2-ethyl-6-methyl-3-hydroxypyridine succinate. At 1, 3, 7, 14, and 28 days after modeling of CST, rats of experimental group 1 had the common bile duct catheterized and bile collected during 1 hour under thiopental sodium anesthesia. The content of total bile acids (TBA) was determined in the obtained portion of bile. In rats of experimental group 2, similar examinations were performed and the rats were taken out of the experiment after 7, 14 and 28 days of the posttraumatic period.
Results and Discussion. Under the influence of CST in rats of different sexes, the concentration of TBA in bile significantly decreased. In female rats, the content of TBA reached a minimum value after 3 days, remained at the same level until day 14, and increased by day 28, reaching the control level. In male rats, the pattern of response of the bile-forming function of the liver was similar, the index reached a minimum value after 7 days, remained at the same level until day 14 and increased until day 28, but did not reach the control level. In the dynamics of CST, the index in male rats after 1, 7, 14 and 28 days was significantly lower compared to female rats. Under the influence of correction, compared with rats without correction, the content of TBA in bile became statistically significantly higher in female rats starting from the 14th day of the experiment, and in male rats – starting from the 7th day of the experiment.
Conclusions. Gender differences in the dynamics of TBA content in bile have been established, the degree of decrease of which under the influence of CST is significantly greater in male rats than in female rats with a maximum of disorders after 7 days of experiment and recovery by 28 days. The use of 2-ethyl-6-methyl-3-hydroxypyridine succinate at a dose of 100 mg·kg-1 in rats of different sexes under conditions of CST causes less disturbances of TBA content in the bile and is more pronounced in male rats, starting from the 14th day of the experiment.
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