THE EFFECT OF INOSITOL ON THE CONTENT OF NITRITE ANION IN THE HEART AND EYES OF DIFFERENT SEXES RATS WITH EXPERIMENTAL STEATOHEPATOSIS

Abstract

Introduction. Steatohepatosis is one of the main problems today, which is often complicated by cardiovascular disorders, impaired blood supply, and retinal pathology. At the same time, cell damage occurs due to the development of oxidative stress, disruption of nitric oxide metabolism. Antioxidants counteract this, including inositol.

The aim of the study – to evaluate changes in the content of nitrite anion (NiA) in the heart and eyes of different sexes rats with steatohepatosis and the effect of inositol preparations on it.

Research Methods. Experiments were performed on 216 white rats of different sexes, at the time of withdrawal from the experiment they were 7.5–8 months old. The animals were divided into 9 groups: 1 – control, 2 – myo-inositol (MI), 3 – D-chiro inositol (DI), 4 – steatohepatosis 2 months, 5 – steatohepatosis 2 months + MI 2 months, 6 – steatohepatosis 2 months + DI 2 months, 7 – steatohepatosis 4 months, 8 – steatohepatosis 4 months+ MI 2 last months, 9 – steatohepatosis 4 months+DI 2 last months. Steatohepatosis was induced by drinking of 20% fructose solution (Fr) for 2 months in groups 4, 5 and 6, and for 4 months in groups 7, 8 and 9. Inositols were administered intragastrically (MI – 400 mg/kg of weight, DI – 30 mg/kg of weight) 2 months after the beginning of simulating steatohepatosis. The content of nitrite anion (NiA) was determined in heart and eye homogenates.

Results and Discussion. In group 1 of rats, the NiA in the heart was higher by 60.9 % (p<0.001) and in the eyes by 6.8 % (p<0.05) in females. MI caused a significant increase in NiA in the heart of males by 6.1 % (p<0.05), and DI – by 3.5 % (p<0.05), in the heart of females only MI increased NiA by 5.4 % ( p<0.05). In the eye homogenate, MI and DI did not cause a significant increase in NiA in males, while in females it increased under the influence of MI by 3.2 % (p<0.05). In groups 2 and 3, higher NiA in females remained in the heart and eyes. In rats with striatohepatosis, an increase in the content of NiA in the heart and eyes was noted, which depended on the duration of Fr use only in females. The effect of Fr on the heart, compared to the eyes, regarding the increase of NiA was greater. In the heart and eyes of males, higher NiA was noted after 2 months, and lower after 4 months. The introduction of MI or DI contributed to a significant reduction of NiA in the heart and eyes, but the return to control values was only in the eyes of males and females of groups 5 and 6. When stopping the use of Fr, MI and DI acted the same. In the heart and eyes of males and females that continued to receive Fr and simultaneously injected MI or DI, a significant decrease in NiA was noted, the return to control values was only in the eyes of males and females.

Conclusion. Steatohepatosis in different sexes rats causes the development of nitrosidative stress in the heart and eyes. In the heart and eyes of females, it directly depends on the duration of fructose consumption, and in males, the content of nitrite anion does not differ reliably after 2 and 4 months of drinking fructose. Myo-inositol and D-chiro-inositol decrease nitrite anion content in rat heart and eyes. When fructose supplementation is stopped, myo-inositol and D-chiro inositol have a better effect on the heart of females. With the introduction of myo-inositol and D-chiro inositol, the content of nitrite anion in the eyes of males and females is completely restored, which does not depend on the duration of fructose consumption.