OBTAINING AND STANDARDIZATION OF LIQUID ROOT EXTRACT GENTIANA АSСLEPIADEA
DOI:
https://doi.org/10.11603/mcch.2410-681X.2024.i2.14636Keywords:
extract, Gentiana аsсlepiadea, biologically active substances, standardization, spectrophotometry, identification, quantification, adaptogenic activityAbstract
Introduction. One of the important directions of pharmacy development is obtaining extraction preparations from new types of plant raw materials to create safe and effective phytopreparations. Liquid extracts are one of the most common extraction dosage forms, the advantages of which are the relative ease of manufacture, the preservation of active substances in their natural form, the convenience of use as a finished product and the possibility of use as part of complex drugs.
The aim of the study – to prepare the liquid extract of the roots of Gentiana аsсlepiadea and to standardize it.
Research Methods. The raw material for obtaining the liquid extract was the roots of Gentiana (G.) аsсlepiadea, which were harvested in Ivano-Frankivsk region in 2020. The liquid extract was obtained by remaceration, and 40% ethyl alcohol was used as an extractant. The content of the total free organic acids, ascorbic acid, oxidizing phenols in the extracts was determined by titrimetric methods; polysaccharides – by gravimetric method; total polyphenols – spectrophotometrically on the ULAB model 102 spectrophotometer (China). Standardization of the obtained extract was carried out in accordance with the requirements of the monograph “Extracts” (State Pharmacopoeia of Ukraine 2.0).
Results and Discussion. The results of the study showed that a liquid extract of the roots of G. аsсlepiadea contains a sum of polyphenols – 3.96±0.07 %, oxidizing phenols – 4.16±0.09 %, polysaccharides – 0.65±0.01 %, organic acids – 0.49±0.01 %, ascorbic acid – 0.034±0.002 %. During standardization, the test extract passed the test according to the main numerical indicators and microbiological purity. The ethanol content in the extract was 39.4±2.88 %, the dry residue – 20.6±0.76 %, the total polyphenols – at least 3%.
Conclusions. The liquid extract of the roots of G. аsсlepiadea was obtained by remaceration using 40 % ethyl alcohol as extractant. The content of the main groups of biologically active substances was identified and quantified. The resulting extract is standardized in accordance with the monograph “Extracts” (State Pharmacopoeia of Ukraine 2.0). The results of the conducted studies indicate the prospects for further study of the adaptogenic activity of the extract from the roots of G. аsсlepiadea.
References
Kukhtenko, O.S., Bevz, N.Y., Gladukh, Ye.V., & Kukhtenko, H.P. (2019). Study on the development of liquid extract of cardiotonic action. Pharmaceutical journal, 3, 41-48 [in Ukrainian].
Bryda, O.R., Stadnytska, N.Ye., Mylyanych, A.O., Maltyz, I.S., & Shalata, V.Ya. (2020). The advantage of the extraction method with constant movement of the extractant on the example of obtaining an extract of the root of Pelargonium sidoides. Chemistry, Technology and Application of Substances, 3 (1), 110-116 [in Ukrainian].
Matsyuk, O.D., Vyshnevska, L.I., & Buhay, A.V. (2021). Overview of the assortment of medicinal extracts on the pharmaceutical market of Ukraine. Social pharmacy in health care, 7 (3), 31-40 [in Ukrainian].
Bernatoniene, J., Kopustinskiene, D.M., Jakstas, V., Majiene, D., Baniene, R., Kuršvietiene, L., … & Trumbeckaite, S. (2014). The effect of Leonurus cardiaca herb extract and some of its flavonoids on mitochondrial oxidative phosphorylation in the heart. Planta Med., 80 (7), 525-532.
Krvavych, A.S., Hamada, V.R., Konechna, R.T., Mylyanych, A.O., Zhurakhivska, L.R., Buchkevych, I.R., & Novikov, V.P. (2019). Extraction of phenolic compounds from Adonis vernalis grass. Questions of chemistry and chemical technology, 5 (126), 54-57 [in Ukrainian].
Perez-Armada, L., Rivas, S., Gonzalez, B., & Moure, A. (2019). Extraction of phenolic compounds from hazelnut shells by green processes. J. Food Eng, (255), 1-8.
Wang, L., Lin, X., Zhang, J.C., Zhang, W.M., Hu, X.P., Li, W., … & Liu S.X. (2019). Extraction methods for the releasing of bound phenolics from Rubus idaeus L. leaves and seeds. Ind. Crop. Prod., 135, 1-9.
Joksić, G., Tričković, J.F., & Joksić, I. (2019). Potential of Gentiana lutea for the treatment of obesity-associated diseases. Current Pharmaceutical Design., 25 (18), 2071-2076.
Nastasijević, B., Lazarević-Pašti, T., Dimitrijević-Branković, S., Pašti, I., Vujačić, A., & Joksić, G. (2012). Inhibition of myeloperoxidase and antioxidative activity of Gentiana lutea extracts. J. Pharm. Biomed. Anal., 66, 191-196.
Berk, A., Yılmaz, İ., Abacıoğlu, N., Kaymaz, M.B., Karaaslan, M.G., & Kuyumcu, Savan E. (2022). Antidepressant effect of Gentiana olivieri Griseb. in male rats exposed to chronic mild stress. Libyan J Med., 15 (1), 1725991.
Marchyshyn, S.M., Stoyko, L.I., & Dakhym, I.S. (2016). Study of the qualitative composition and quantitative content of hydroxycinnamic acids in Gentiana cruciata L. Pharmaceutical journal, 3-4, 76-81 [in Ukrainian].
Hrodzinskyy, A. M. (Ed.). (1990). Medicinal plants: Encyclopedic reference. Kyiv: Chairman. ed. URE, 120-121 [in Ukrainian].
State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine (Vol. 3, 2nd ed.)]. (2014). Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality [in Ukrainian].
State Register of Medicinal Products of Ukraine. Retrieved from http://www.drlz.com.ua
Aggregator of pharmacies, medical preparations, hygiene, health and beauty products. Retrieved from https://tabletki.ua/
State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine (Vol. 1, 2nd ed.)]. (2015). Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality [in Ukrainian].
Huzio, N., Grytsyk, A., Raal, A., Grytsyk, L., & Koshovyi, O. (2022). Phytochemical and pharmacological research in Agrimonia eupatoria L. herb extract with anti-inflammatory and hepatoprotective properties. Plants, 2(11), 2371.
Romanenko, Ye. A., Koshovyi, O. M., Komisarenko, A. M., & Shtryhol, S. Yu. (2015). Phytochemical study of liquid extract of St. Nettle herb and investigation of its psychotropic activity. Coll. of science works of employees of NMAPO named after P. L. Shupyka, 24 (5), 212-218 [in Ukrainian].
Grytsyk, A., Dubel, N., & Grytsyk, L. (2021). Research of extraction parameters of Alchemilla Subcrenata bus. herb. SSP Modern Pharmacy and Medicine, 1 (2), 1-9.
Hrytsyk, N.Yu., & Erstenyuk, H.M. Study of the acute toxicity of extracts of Gentiana asclepiadea. Art of Medicine, 2023, 2(26), 29-34 [in Ukrainian].
Hrytsyk, N.Yu., & Erstenyuk, H.M. (2022). The study of the hepatoprotective activity of the extracts of Gentiana asclepiadea on the model of acute tetrachloromethane hepatitis. Art of Medicine, 2022, 4 (24), 21-26 [in Ukrainian].
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
