VITAMIN D LEVELS IN BLOOD SERA OF TERNOPIL REGION RESIDENTS WITH LYME BORRELIOSIS AND CHRONIC EPSTEIN-BARR VIRUS INFECTION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2024.i1.14598Keywords:
Lyme borreliosis, chronic Epstein-Barr virus infection, serum vitamin D level, indirect immunofluorescence reaction, BIOCHIP technology, polymerase chain reaction, enzyme-linked immunosorbent assay, immunoblotAbstract
Introduction. Scientists suggest that vitamin D, can control the antiviral immune response. Therefore, it is necessary to find out its management in patients with viral and bacterial mix infections.
The aim of the study – to determine the level of 25(OH)D in the blood sera of Ternopil region residents with Lyme borreliosis (LB) and chronic Epstein-Barr virus infection (EBV) separately and combined.
Research Methods. 81 patients with clinical manifestations of LB and EBV were examined. LB was diagnosed clinically and confirmed serologically (ELISA and immunoblot). Chronic EBV was diagnosed by indirect immunofluorescence, BIOCHIP technology. The active phase of the EBV was determined by the high level of viral DNA in both patients blood and saliva by PCR method. The level of 25(OH)D was determined by chemiluminescent immunoassay. The results were interpreted according to the recommendations of the First Ukrainian Consensus on Vitamin D Management (2023): <20 ng/ml – vitamin D deficiency (VDD); ≥20–29 ng/ml – vitamin D insufficiency (VDI); >30–50 ng/ml – sufficient vitamin D level (SDA); >50–60 ng/ml – safe but not target vitamin D level (SBTVD); >60–100 ng/ml – zone of uncertainty with potential benefits or risks; >100 ng/ml – excess/zone of vitamin D toxicity.
Results and Discussion. VDD and VDI (combined) were more frequently recorded in patients with chronic EBV in the active phase, combined with LB, and only for this disease compared with patients with EBV in the latent phase separately, p<0.05. SDA and SBTVD (combined) were more prevalent in patients with chronic EBV in the latent phase separately or in combination with LB compared with patients with EBV in the active phase with LB or separately, p<0.05. The mean 25(OH)D level was higher in patients with chronic EBV in the latent phase than in patients with the active phase of EBV, both with and without LB, p<0.05, and higher in patients with chronic EBV in the latent phase in combination with LB compared with patients with active EBV separately, p<0.05.
Conclusions. Determination of vitamin D level in Ternopil region residents with LB and chronic EBV by 25(OH)D level was performed first time and allowed to establish a significant difference in its content in patients with EBV in the active or latent phases, as well as in combination with LB.
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