DETERMINATION OF THE CONTENT OF HYDROXYCINNAMIC AND ORGANIC ACIDS IN THE LEAVES OF SMOOTH HYDRANGEA
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i4.14380Keywords:
leaves of Smooth Hydrangea, Hydrangea arborescens L., organic acids, hydroxycinnamic acids, quantitative content, spectrophotometry, alkalimetric titrationAbstract
Introduction. Smooth Hydrangea (Hydrangea arborescens L.) is a promising plant raw material for phytochemical and pharmacological research. It is the most common and unpretentious representative of the genus Hydrangea L., which displays significant biological activity. It is not included in the State Pharmacopoeia of Ukraine. There are no pharmaceutical products based on this plant in the domestic pharmaceutical market. However, Hydrangea arborescens L. contains a lot of biologically active compounds and exhibits medicinal properties, as evidenced by its use in folk medicine and homeopathy. The relevance of this study is due to the insufficient study of the chemical composition and pharmacological properties of the leaves of Smooth Hydrangea.
The aim of the stdy – to determine the weight loss during drying and to establish the quantitative content of hydroxycinnamic and organic acids in the leaves of Hydrangea arborescens L.
Research Methods. The object of the research were leaves of Smooth Hydrangea. Physico-chemical (absorption spectrophotometry in the UV region of the spectrum), chemical (gravimetry, alkalimetry) and statistical methods (mathematical processing of the experimental results) were used for research. In the course research, water and water-alcohol extracts of the leaves, class A measuring dishes, chemical reagents that meet the requirements of the SPhU, a water bath, a drying cabinet, an desiccator, analytical balance "Radwag AS 220/C", and spectrophotometer Schimadzu 1800-UV (Japan) were used.
Results and Discussion. The loss in weight after drying the leaves of Hydrangea arborescens L., which was determined by the gravimetric method and amounted to (9.12±0.25) %. The quantitative content of the sum of free organic acids in terms of malic acid was determined by the alkalimetry and amounted to (1.67±0.05) %. The quantitative content of the sum hydroxycinnamic acids in terms of chlorogenic acid and absolutely dry raw materials was determined by absorption spectrophotometry in the UV region of the spectrum and amounted to (2.34±0.03) %.
Conclusions. The determined percentage of organic acids (1.67±0.05 )% and hydroxycinnamic acids (2.34±0.03) % in the leaves of Hydrangea arborescens L. gives reason to consider the plant as a promising raw material for further research.
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