STUDY OF THE COMPONENT COMPOSITION OF THE PHENOLIC COMPOUNDS OF THE HERB ASTRAGALUS DASYANTHUS PALL.
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i3.14137Keywords:
Astragalus dasyanthus Pall., herb, hydroxycinnamic acids, flavonoidsAbstract
Introduction. The genus Astragalus of the Fabaceae family is one of the most numerous and has 2.500–3.500 species. Astragalus dasyanthus Pall. (A. dasyanthus) is a perennial herbaceous plant, the plant material of which is grass. The chemical composition of plant raw materials is quite diverse. There are polysaccharides, mucus, organic acids, triterpene saponins, flavonoids, mineral compounds. The herb extract is a popular hypotensive, sedative, vasodilator, diuretic, antiedematous, hemostatic, antiemetic agent. The plant raw materials of domestic production are practically not studied. Therefore, conducting a pharmacognostic study of the herb A. dasyanthus was relevant.
The aim of the study – to determine the component composition of hydroxycinnamic acids and flavonoids in the domestically harvested herb of A. dasyanthus.
Research Methods. Series of A. dasyanthus grass were harvested during 2020–2021 from private plots in Ukraine. The component composition of hydroxycinnamic acids and flavonoids was determined by HPLC on an Agilent Technologies 1200 liquid chromatograph.
Results and Discussion. The component composition of the herb A. dasyanthus, which is represented by 5 hydroxycinnamic acids and 8 flavonoids, was studied using the HPLC method. The chlorogenic acid (615.88±1.68) μg/g was dominated by quantitative content from the identified hydroxycinnamic acids and the content of neohesperidin (1885.06±1.04) μg/g and rutin (1390.15±17.41) µg/g was dominated from identified flavonoids.
Conclusions. The component composition of hydroxycinnamic acids and flavonoids of the herb A. dasyanthus harvested in Ukraine was studied using HPLC. It was established that the dominant component of hydroxycinnamic acids of the herb A. dasyanthus is chlorogenic acid (615.88±1.68) μg/g and flavonoid compounds are neohesperidin (1885.06±1.04) μg/g and rutin (1390.15±17.41) μg/g. A promising direction of further research is the study of the component composition of phenolic compounds of the A. dasyanthus herb series harvested in different regions of Ukraine and from plants of different ages.
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