DETERMINATION OF QUANTITATIVE CONTENT OF POLYSACCHARIDES IN LIQUID EXTRACT OF CREEPING THYME, CHOICE OF QUANTITATIVE QUALITY CRITERIA

Abstract

Introduction. Creeping thyme is a valuable medicinal plant characterized by a complex of biologically active substances (BAS) with versatile pharmacological activity, in particular mucolytic, for which, according to literature sources, such BAS as: flavonoids, amino acids, components of essential oil, etc., are responsible. Previous studies, we have established the presence of polysaccharides in extracts from creeping thyme grass, obtained with water and solutions with low alcohol concentrations. Since, pharmaceutical development on the basis of a thick extract of creeping thyme and essential oil of thyme ordinary, provided, first of all, the production of liquid and thick extracts from PE, therefore, the creation of a new domestic mucolytic agent based on two types of thyme requires, precisely, the study of the analyzed BAS not only in medicinal plant materials, but also in the obtained extracts, in order to trace the presence and reproducibility of BAS of the studied raw materials and offer appropriate indicators and quality criteria.

The aim of the study – to determine the quantitative content of polysaccharides in the liquid extract of СТ, the choice of quantitative quality criteria.

Research Мethods. The quantitative determination of polysaccharides in the studied extract was carried out by gravimetry. For the study, liquid extract of creeping thyme, chemical reagents, water bath, vacuum filter, drying cabinet, desiccator, analytical weight were used.

Results and Discussion. The methodological approach to the standardization of liquid extract of creeping thyme was based on the identification and quantitative determination of similar BAS raw materials of СТ, continuing the proposed approach: medicinal plant raw materials – liquid extract – thick extract – finished medicinal product. The quantitative content of polysaccharides was determined by gravimetry, which is accurate, but long-term and labor-intensive. To do this, first select the necessary precipitator, carry out the deposition process itself, after which the resulting precipitate was filtered, washed, dried at the required temperature, cooled in a desiccator and weighed on analytical scales. As a result of the experiment, the content of polysaccharides in the liquid extract of the СТ was determined, the correlation of their content was noted, and the quantitative criterion for the quality of the liquid extract was chosen – the content of polysaccharides, which is (0.26–0.30 %).

Conclusions. The method of gravimetry determined the quantitative content of polysaccharides in the liquid extract of creeping thyme, noted the correlation of their content, established a quantitative quality criterion – the content of polysaccharides (0.26–0.30 %).