THE EFFECT OF L-ARGININE AND AMINOGUANIDINE ON THE DEVELOPMENT OF OXIDATIVE STRESS IN THE LUNGS IN EXPERIMENTAL ANTIPHOSPHOLIPID SYNDROME
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i4.13573Keywords:
antiphospholipid syndrome, oxidative stress, L-arginine, aminoguanidineAbstract
Introduction. Antiphospholipid syndrome is an autoimmune disease accompanied by the formation of antiphospholipid antibodies which entering into blood coagulation reactions, cause thrombosis of veins and arteries, as well as contribute to miscarriage. At the same time, the processes of free radical oxidation are activated, which is a consequence of ischemia, the antioxidant system is depleted, which deepens lung damage.
The aim of the study – to investigate the state of the prooxidant-antioxidant system in the lung tissue with experimental APS and when using modulators of nitric oxide synthesis (L-arginine and aminoguanidine).
Research Methods. Modeling of the antiphospholipid syndrome was performed on female BALB/c mice. The level of oxidation products and the activity of antioxidant enzymes were determined under APS and with the use of L-arginine and aminoguanidine in the lung tissue.
Results and Discussion. Under conditions of experimental APS in the lungs of mice, an increase in the content of lipid hydroperoxides and TBA-active products, inhibition of the activity of superoxide dismutase and catalase, depletion pool of the reduced glutathione, and impaired function of respiratory chain enzymes – succinate dehydrogenase and cytochrome oxidase. Positive dynamics was noted in restoring the activity of the antioxidant system and blocking the destructive action of free radicals on the lung tissue with the introduction of L-arginine and aminoguanidine and their combination.
Conclusions. Thrombosis and ischemia that occur during APS disrupt the balance of the prooxidant-antioxidant system, increasing the formation of reactive oxygen species and suppressing protective functions. The combined use of L-arginine and aminoguanidine in antiphospholipid syndrome helps to reduce the manifestations of oxidative stress in the lungs, in particular, to reduce the level of lipid peroxidation, restore the activity and content of the components of the antioxidant system, which is accompanied by the restoration of the activity of respiratory chain enzymes.
References
Janowiak P., Siemińska A., Porzezińska M., Smoleńska Z., Suchanek H., Jassem E. (2018). Difficulties in the treatment of recurring diffuse alveolar hemorrhage accompanying primary antiphospholipid syndrome: a case report and literature review. Advances in Respiratory Medicine, 86, 126-130.
Vaz, C.O., Mazetto, B.M., Vasconcelos P.E.N.S., Bastos L.B., Aparecida Cursino, M., Quintanilha, J.C.F., et al. (2021). Association between plasmatic oxidative stress and thrombosis in primary antiphospholipid syndrome. Journal of Thrombosis and Thrombolysis, 52, 730-737. https://doi.org/10.1007/s11239-021-02509-0
Yaremchuk, O.Z., Posokhova, K.A., Kuzmak, I.P., Kulitska, M.I., Klishch, I.M., Korda, M.M. (2020). Indexes of nitric oxide system in experimental antiphospholipid syndrome. Ukrainian Biochemical Journal, 92 (1), 75-83. ISSN 2409-4943
Knight, J.S., Kanthi, Y. (2022). Mechanisms of immunothrombosis and vasculopathy in antiphospholipid syndrome. Seminars in Immunopathology, 44, 347-362.
Chaturvedi, S., Brodsky, R.A., McCrae, K.R. (2019). Complement in the pathophysiology of the antiphospholipid syndrome. Front. Immunol., 10, 449. DOI: 10.3389/fimmu.2019.00449
Maioli G., Calabrese G., Capsoni F., Gerosa M., Meroni P.L., Chighizola C.B. (2019). Lung Disease in Antiphospholipid Syndrome. Semin. Respir. Crit. Care Med., 40 (2), 278-294. DOI: 10.1055/s-0039-1683994.
Forman, H.J., & Zhang, H. (2021). Targeting oxidative stress in disease: promise and limitations of antioxidant therapy. Nature Reviews Drug Discovery, 20, 689-709.
Delgado-Roche, L., Mesta, F. (2020). Oxidative stress as key player in severe acute respiratory syndrome coronavirus (SARS-CoV) infection. Archives of Medical Research, 51 (5), 384-387.
Nocella, C., Bartimoccia, S., Cammisotto, V., D’Amico A., Pastori, D., Frati, G., Sciarretta, S., Rosa, P., Felici, C., Riggio, O., Calogero, A., Carnevale, R. (2021). Oxidative stress in the pathogenesis of antiphospholipid syndrome: Implications for the atherothrombotic process. Antioxidants, 10 (11), 1790. https://doi.org/10.3390/antiox10111790
Mamenko, T.P., Kots, S.Ya. (2020). Peroxide oxidation of lipids of cell membranes in the formation and regulation of protective reactions of plants. Ukrainian Botanical Journal, 77 (4), 331-343. https://doi.org/10. 15407/ukrbotj77.04.331 [in Ukrainian].
Garasym, N., Verbeshchuk, M., Bodnarchuk, N., Galan, M., Sanagurskyi, D. (2020). Intensity of free radical processes in blood plasma of rats under the influence of histamine and quercetin. Bulletin of Lviv University. Biological Series, 82, 36-52. DOI: http://dx.doi.org/10. 30970/vlubs.2020.82.03 [in Ukrainian].
ABlair, I. (2001). Lipid hydroperoxide-mediated DNA damage. Experimental Gerontology, 36, 1473-1481. https://doi.org/10.1016/S0531-5565(01)00133-4
Tsekhmistrenko, S.I., Tsekhmistrenko, O.S., Polishchuk, V.M. (2010). The effect of different forms of selenium on indicators of lipid peroxidation in the kidneys of quail under cadmium load. Scientific Bulletin of Veterinary Medicine, 6 (79), 142-145 [in Ukrainian].
Tyazhka, O.V., Zagorodnya, Y.M. (2016). State of lipid peroxidation and antioxidant system in children of different ages. Perinatology and Pediatrics, 2 (66), 101-105. DOI 10.15574/PP.2016.66.101 [in Ukrainian].
Govorukha, O.Yu., Shnaiderman, O.Yu. (2016). The importance of the interaction of lipid peroxidation and antioxidant systems in the development of pathological processes. Experimental and Clinical Medicine, 4 (73), 10-14 [in Ukrainian].
Guberuk, V.O. (2008). Peroxidation of lipids and the antioxidant system of the body's protection. Scientific Bulletin of Gzhitskyi, 10, 3 (38) Part 1, 51-55 [in Ukrainian].
Antonova, O.I., Lutsenko, B.O., Shapoval, A.V. (2014). Effects of the complex action of lack, excess of melatonin and increased peroxidation on the liver. South Ukrainian Medical Scientific Journal, 9, 58-61 [in Ukrainian].
Yaremchuk, O.Z., Posohova, K.A., Kulytska, M.I. (2020). Influence of L-arginine and aminoguanidine on the development of oxidative stress in the brain in experimental antiphospholipid syndrome. Sciences of Europe, 3 (48), 20-24 [in Ukrainian].
Posohova, K.A., Sak, I.U., Sampara, S.R. (2014). Obstetric antiphospholipid syndrome and the nitric oxide system (literature review and results of own research). Medicinal Chemistry, 16 (1), 73-80 [in Ukrainian].
Zaychenko, H.V., Laryanovska, Yu.B., Deyeva, T.V. (2011). The morphological state of the uterus and placenta during experimental modeling of gestational antiphospholipid syndrome in mice. Ukrainian Medical Almanac, 14 (4), 136-141 [in Ukrainian].
Chevari, S., Chaba, I., Sekey, I. (1985). The role of superoxide dismutase in oxidative processes of the cell and the method of its determination in biological materials. Lab. Case, 11, 678-681 [in Russian].
Korolyuk, M.A., Ivanova, L.I., Mayorova, I.G. (1988). Method for determining catalase activity. Lab. Case, 1, 16-19 [in Russian].
Ellman, G.L. (1959). Tissue sulfhydryl groups. Arch. Biochem. Biophys., 82, 70-77 [in Russian].
Gavrilov, V.B., Myshkorudnaya, M.I. (1983). Spectrophotometric determination of lipid hydroperoxide content in blood plasma. Lab. Case, 3, 33-35 [in Russian].
Andreeva, L.I., Kozhemyakin, A.A. (1988). Kishkun modification of the method for determining lipid peroxides in a test with thiobarbituric acid. Lab. Case, 11, 41-43 [in Russian].
Eshchenko, H.G. Volsky, N.D. (1982). Determination of the amount of succinic acid and the activity of succinate dehydrogenase. Methods of biochemical research. L.: Izd-vo Leningrad. University, 207-210 [in Russian].
Kryvchenkova, R.S. (1977). Determination of cytochrome oxidase activity in mitochondrial suspension. Modern methods in biochemistry. Orehovicha, V.N. (Ed.). Moscow: Meditsina [in Russian].
Lowry, O.M., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193 (1), 265-275 [in Russian].
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
