IMMUNO-PHENOTYPIC PROPERTIES OF PERIPHERAL BLOOD LYMPHOCYTES OF INFERTILITY MEN WITH COMORBIDITIES
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i2.13088Keywords:
infertility, idiopathic infertility, rheumatoid arthritis, blood serum, population composition of lymphocytesAbstract
Introduction. It is known that approximately 15 % of married couples suffer from infertility, with the malefactor accounting for almost 50 % of cases. According to the traditional method of evaluation, in 30 % of patients, the etiology of the male contribution to infertility remains unexplained and, thus, is identified as idiopathic. Therefore, the diagnosis of male infertility cannot be limited only a simple sperm analysis. The regulation of male reproductive function is multi-level, involving not only the endocrine system, but also the immune system. Male infertility is also observed with other accompanying pathologies, in particular, rheumatoid arthritis. For a better understanding of the immunopathogenic mechanisms of infertility, it is necessary to study the role of various immune factors.
The aim of the study – to evaluate the population composition and activation markers of peripheral blood lymphocytes of men with idiopathic infertility and autoimmune joint pathology.
Research Methods. The research was carried out on blood serum and seminal plasma of 45 infertile men aged 22–48: with idiopathic infertility (n=23) and autoimmune joint pathology – rheumatoid arthritis (n=22). 27 fertile healthy men of the same age were included in the control group. To determine the quantitative parameters of lymphocytes of the main populations and subpopulations in the blood of patients with idiopathic infertility and autoimmune pathology of the joints, the method of flow cytometry with monoclonal antibodies labeled with fluorescein (BD Biosciences, USA) was used. The number of lymphocytes was determined on a flow cytometer BD FacsCalibur (Becton Dickinson, USA).
Results and Discussion. It was shown that the number of lymphocytes of subpopulations practically did not differ in infertile men from similar indicators of the control group in terms of absolute values. However, it was found that in men with idiopathic infertility, there is a probable increase in the content of CD4+CD25+ T-lymphocytes in the blood, from (0.357±0.185) to (0.504±0.220) G/l (р<0.05). No significant changes were found in autoimmune pathology. Infertile men with autoimmune pathology are distinguished from controls by increased numbers of CD8+ cells and activated T-lymphocytes. The most deviations from control were registered in persons with idiopathic infertility: a reduced number of naive CD4+CD25– lymphocytes with a simultaneous increase in the number of CD4+CD25+ regulatory T-cells, which indicates the conversion of naive T-lymphocytes into regulatory cells.
Conclusions. It was found that the development of infertility against the background of various accompanying pathologies is accompanied by changes in indicators of both systemic and local immune reactivity. The nature of these changes differs depending on the accompanying pathology. The study of the characteristic signs of immune reactivity disorders in infertile men with comorbidities make it possible to position some of them as reliable markers for predicting reproductive function disorders.
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