DETERMINATION OF QUANTITATIVE CONTENT OF AMINO ACIDS IN LIQUID EXTRACT OF CREEPING THYME, CHOICE OF CRITERIA OF ACCEPTABILITY

Abstract

Introduction. According to the literature, it is known that creeping thyme (CT) contains a diverse complex of biologically active substances (BAS). Important flavonoids, phenolic acids, polysaccharides characterized by anti-inflammatory, mucolytic, secretolytic, antiviral and antimicrobial action are among the BAS of thyme herb. Previous studies have also established the presence of amino acids in raw materials that perform important functions in the human body. In recent years, the number of studies related to their study has increased significantly. Pharmaceutical development on the basis of thick extract of CT and essential oil of thyme involved obtaining a liquid extract of CT, which was obtained by a suitably selected method with maximum preservation and reproduction of BAS of this medicinal plant material. In the previous work, we covered the issue of identification of amino acids in the studied extract and selected the identification marker of the quality of liquid extract of CT – glycine. However, for its standardization it is necessary to determine not only their qualitative composition, but also the quantitative content, which will further offer indicators of quality of liquid extract of the state of emergency and choose the appropriate eligibility criteria.

The aim of the study – quantitative determination of amino acids in liquid extract of CT, selection of appro­priate acceptance criteria.

Research Мethods. Spectrophotometer "Cary-50", liquid extract of creeping thyme, FSЕ: glycine (Sigma), 0.2 % ninhydrin solution R, water bath.

Results and Discussion. Determination of amino acids in the studied extract of creeping thyme was performed by spectrophotometry in the visible part of the spectrum using the known and used photometric reaction to form a colored compound of amino acids with ninhydrin. It was found that the content of amino acids varies within certain limits and is determined by their content in the raw material. Therefore, in order to standardize the liquid extract of CT, you can choose a quantitative quality criterion – the content of amino acids and offer appropriate acceptance criteria – not less than 0.03 % in terms of glycine.

Conclusions. Quantitative determination of amino acids in liquid extract of CT was performed. As a result of the experiment, their quantitative content was determined and appropriate eligibility criteria were proposed.