MORPHO-FUNCTIONAL CHANGES OF RATS’ OVARY UNDER THE INFLUENCE OF LEAD ACETATE IN THE EXPERIMENT
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i4.12742Keywords:
ovary, female rats, lead, intoxication, morphological changes, biochemical changes in bloodAbstract
Introduction. Lead is a natural toxic metal found in the earth's crust. As a toxicant it is in the focus of attention not only of ecologists, toxicologists and hygienists, but also morphologists and clinicians. This is due to the fact that poisoning by lead compounds is accompanied by dysfunction of the nervous, cardiovascular, digestive, urinary and reproductive systems.
The aim of the study – to learn the effect of low, medium and high doses of lead acetate on biochemical parameters and morphological status of rat ovaries in the experiment.
Materials and Methods. The study was performed on 40 nonlinear female rats, divided into 4 experimental groups. Rats were given 30 days to drink a solution of lead acetate at a rate of 0.05 mg/kg, 10 mg/kg and 60 mg/kg of animal weight. Biochemical, histological and statistic metods were used in the work.
Results and Discussion. It was found that in group III of animals, compared with II, DC were 2.8 times higher, TBA-active products were observed more than 2.1 times. In group IV of animals in comparison with group II, DCs were 4.3 times higher, TB-active products were detected 2.7 times more. In group IV of animals in comparison with group III of animals, DCs were higher by 55.8 %, TB-active products were found by 24.8 %. It was found that in group III animals, compared with II, SOD was lower by 34.7 %, and catalase activity was higher by 32.3 %. In group IV animals, compared with group II, SOD was lower by 63 %, catalase activity – by 86.6 %. In group IV animals, compared with group III, SOD was lower by 43.3 % and catalase activity – by 89.9 %. As the dose of lead acetate increases, morphological changes in both cortex and medulla of rat ovaries deepen, which leads to changes in the thickness of ovarian surface structures and a decrease in the number of follicles, indicating impaired growth and maturation.
Conclussions. Under the influence of small doses of lead acetate changes have adaptive character, and under the large doses – degenerative.
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