NITRITE-ANIONS CONTENT CHANGES IN THE VENTRICLES AND ATRIA MYOCARDIUM OF RATS UNDER CONDITIONS WITH DEVELOPMENT OF ELECTROLYTE-STEROID CARDIOMYOPATHY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i3.12586Keywords:
heart, NO2, dexamethasone, salt, L-carnitineAbstract
Introduction. Arterial hypertension and metabolic disorders are often the result of long-term steroid treatment. Eating a diet with high NaCl level can significantly increase this risk. A serious complication due to these disorders is often the development of cardiomyopathy. One of the preventive measures in this case is the nitric monooxide system. L-carnitine, which is an antioxidant, has the ability to influence energy metabolism, apoptosis processes, DNA transcription and it can be a promising means of correcting disturbances in the nitric monooxide system.
The aim of the study – to learn the content of NO2- in the heart of rats under prolonged exposure to dexamethasone, an increased content of NaCl (4 %) in drinking water and under correction with L-carnitine.
Research Methods. The experiment was carried out on 96 white nonlinear rats in compliance with the bioethics standards. The content of nitrite anion was determined in homogenates of the atria and ventricles by the Gris method. Steroid cardiomyopathy was modeled by daily dexamethasone administration for 15 days. Half of the animals received a 4 % NaCl solution in tap water for drinking. Correction was performed with L-carnitine given per OS.
Results and Discussion. It was found that long-term use of dexamethasone and high salt content in water lead to a decrease in NO2- levels in the heart ventricles of rats of both sexes (24 %, p<0.05). L-carnitine demonstrates the ability to restore metabolite levels. The difference between the sexes appears in animals with normal NaCl levels in water. With a high level of salt in the water, the negative changes are more intense and the difference between the sexes is no longer so pronounced. In the atria, only a difference in the content of NO2- between the sexes was found (13 % (p<0.05) lower in males than in females at all stages of the study). Dexamethasone or salt does not affect the metabolite in this tissue.
Conclusions. Steroid-electrolyte damage of the heart is accompanied with a decrease in nitrite anion in the ventricular myocardium, L-carnitine effectively neutralizes these changes. In the atrial myocardium, a difference in the content of nitrite anion was found only depending on gender; dexamethasone or high salt content did not affect this indicator.
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