CHANGES IN THE CONTENT OF MACROELEMENTS IN THE BONE OF THE TEMPОROMANDIBULAR JOINT AT ITS DIABETIC DAMAGE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i3.12580Keywords:
temporomandibular joint, diabetic arthropathy, macroelements, temporal bone, mandibleAbstract
Introduction. Joint damage in patients with diabetes is a fairly common complication. Peculiarities of the macroelements composition of the temporal bone and mandible and structural changes of the temporomandibular joint in diabetes have been insufficiently studied.
The aim of the study – to learn the features of the macroelements composition of the temporal bone and mandible at diabetic arthropathy of the temporomandibular joint.
Research Methods. Atomic absorption spectral analysis examined the content of macroelements (calcium, phosphorus, magnesium, sodium), the ratio of calcium to phosphorus in the temporal bone of the mandible of 45 laboratory adult white male rats, which were divided into 3 groups. Group 1 consisted of 15 intact almost healthy animals, group 2 – 15 rats with monthly diabetic arthropathy of the temporomandibular joint, group 3 – 15 animals with two-month specified pathology. Histological micronutrients were also made of the joint, temporal bone and mandible, on which the relative volumes of damaged chondrocytes and synoviocytes were determined, and the relative volumes of osteoblasts and osteoclasts in bone tissue. A correlation analysis was performed between the studied morphometric parameters and the content of macroelements. Diabetes mellitus was simulated by a single intraperitoneal injection of streptozotocin by Sigma at a dose of 50 mg/kg. Quantitative indicators were processed statistically.
Results and Discussion. It was established that in diabetic arthropathy of the temporomandibular joint the content of phosphorus, calcium and magnesium decreases disproportionately and unevenly, sodium content increases in the bone tissue of the temporal bone and mandible. It was found that the calcium content in the temporal bone in monthly diabetic arthropathy decreased by 4.8 %, and in the mandible – by 8.8 %, in two-month arthropathy, the studied indicators changed by 8.6 % and 11.6 %, respectively (p < 0.001, phosphorus – by 10 %, 15.4 %, 14.7 % and 18.9 %, respectively. The magnesium content in monthly diabetic arthropathy of the temporomandibular joint in the temporal bone decreased by 6.1 %. in the mandible – by 11.4 %, with a two-month course of simulated pathology – by 15.8 % and 21.8 %, respectively (p<0.001). The sodium content in the temporal bone and mandible under the studied experimental conditions increased. In simulated hyperglycemia, the Ca/P ratio varied significantly and depended on the duration of diabetic arthropathy and dominated in mandible.
Conclusions. Diabetic arthropathy of the temporomandibular joint leads to an uneven, disproportionate decrease in calcium, phosphorus, magnesium and an increase in sodium in the temporal bone and mandible. The most pronounced imbalance between the content of the studied macroelements was found in two-month diabetic arthropathy of the temporomandibular joint and in the mandible. Macroelements imbalance in the temporal bone and mandible in experimental diabetes mellitus affects the disruption of chondrogenesis, mineralization and osteogenesis.
References
Орленко В. Л. Гормональні характеристики діабет-асоційованих остеоартритів // Вісн. проблем біології та медицини. – 2020. – Вип. 2 (159). – С. 138–143.
Кризина О. В. Трофічні порушення тканин нижніх кінцівок при цукровому діабеті 2 типу // Клініч. ендокринологія та ендокринна хірургія. – 2018. – № 1 (6). – С. 15–24.
Лукьянцева Г. В. Особенности химического состава костей белых крыс после двухмесячного употребления натрия бензола и возможности его коррекции // Укр. морфол. альм. – 2014. – 12, № 4. – С. 61–66.
Berenbaum F. Diabetes induced osteoarthritis from a new paradigm to a new phenotype / F. Berenbaum. – Ann. Rheum. Dis. – 2011. – 70 (8). – P. 1354–1356.
Брицке Э. М. Атомно-абсорбционный спектральный анализ / Э. М. Брицке. – М. : Химия, 1982. – 244 с.
Горальський Л. П. Основи гістологічної техніки і морфофункціональні методи дослідження в нормі і при патології / Л. П. Горальський, В. П. Хомич, О. І. Кононський. – Житомир : Полісся, 2011. – 288 с.
Автандилов Г. Г. Основы количественной патологической анатомии / Г. Г. Автандилов. – М. : Медицина, 2002. – 240 с.
Гжибовский А. И. Сравнение количественных данных двух парных выборок с использованием программного обеспечения Statistika i SPSS: параметрические и непараметрические критерии / А. И. Гжибовский, О. И. Иванов, М. А. Горбатова // Наука и здравоохранение. – 2016. – № 3. – С. 5–25.
Резніков О. Г. Загальні етичні принципи експериментів на тваринах / О. Г. Резніков // Ендокринологія. – 2003. – № 8 (1). – С. 142–145.
Мікроелементний склад довгих та мішаних кісток скелета в нормі / Є. В. Гусак, М. В. Погорелов, Г. Ф. Ткач, С. М. Данильченко // Укр. морфол. альм. – 2010. – 8, № 4. – C. 51–55.
Зміни хімічного складу кісток щурів під впливом жовтого барвника тартразину / Г. В. Лук’янцева, В. А. Пастухова, О. Г. Ковальчук, У. М. Дутчак // Світ медицини та біології. – 2019. – № 4 (70). – С. 203–208.
REFERENCES
Orlenko, L. (2020). Hormonalni kharakterystyky diabet-asotsiiovanykh osteoartrytiv [Hormonal characteristics of diabetes-associated osteoarthritis]. Visnyk problem biolohii ta medytsyny – Bulletin of Problems of Biology and Medicine, 2 (159), 138-143 [in Ukrainian].
Kryzyna, O.V. (2018). Trofichni porushennia tkanyn nyzhnikh kintsivok pry tsukrovomu diabeti 2 typu [Trophic disorders of the tissues of the lower extremities in type 2 diabetes]. Klinichna endokrynolohiia ta endokrynna khirurhiia – Clinical Endocrinology and Endocrine Surgery, 1 (6), 15-24 [in Ukrainian].
Lukyantseva, G.V. (2014). Osobennosti khimicheskogo sostava kostey belykh krys posle dvukhmesyachnogo upotrebleniya natriya benzola i vozmozhnosti yego korrektsii [Features of the chemical composition of the bones of white rats after two months of use of benzene sodium and the possibility of its correction]. Ukrainskyi morfolohichnyi almanakh – Ukrainian Morphological Almanac, 12 (4), 61-66 [in Ukrainian].
Berenbaum, F. (2011). Diabetes induced osteoarthritis from a new paradigm to a new phenotype. Ann. Rheum. Dis., 70 (8), 1354-356.
Britske, E.M. (1982). Atomno-absorbtsionnyy spektralnyy analiz [Atomic absorption spectral analysis]. Moscow: Khimiya [in Russian].
Horalskyy, L.P., Khomych, V.P., & Konopskyy, O.I. (2011). Osnovy histolohichnoi tekhniky i morfofunktsionalni metody doslidzhennia v normi i pry patolohii [Fundamentals of histological technique and morphofunctional research methods in normal and in pathology]. Zhytomyr: Polissia [in Ukrainian].
Avtandylov, G.G. (2002). Osnovy kolichestvennoy patologicheskoy anatomii [Basis of quantative pathological anatomy]. Moscow: Meditsyna [in Russian].
Gzhibovskiy, A.I., Ivanov, O.I., & Gorbatova, M.A. (2016). Sravneniye kolichestvennykh dannykh dvukh parnykh vyborok s ispolzovaniyem programmnogo obespecheniya Statistika i SPSS: parametricheskiye i neparametricheskiye kriterii [Comparison of quantitative data of two paired samples using Statistika i SPSS software: parametric and nonparametric criteria]. Nauka i zdravookhraneniye – Science and Healthcare, 3, 5-25 [in Ukrainian].
Reznikov, O.H. (2003). Zahalni etychni pryntsypy eksperymentiv na tvarynakh [General ethical principles of animal experiments]. Endokrynolohiia – Endocrinology, 8 (1), 142-145 [in Ukrainian].
Husak, Ye.V., Pohorelov, M.V., Tkach, H.F., & Danylchenko, S.M. (2010). Mikroelementnyi sklad dovhykh ta mishanykh kistok skeleta v normi [Microelement composition of long and mixed skeletal bones in norm]. Ukrainskyi morfolohichnyi almanakh – Ukrainian Morphological Almanac, 8 (4), 51-55 [in Ukrainian].
Lukyantseva, H.V., Pastukhova, V.A., Kovalchuk, O.H., & Dutchak, U.M. (2019). Zminy khimichnoho skladu kistok shchuriv pid vplyvom zhovtoho barvnyka tartrazynu [Changes in the chemical composition of rat bones under the influence of the yellow dye tartrazine]. Svit medytsyny ta biolohii – The World of Medicine and Biology, 4 (70), 203-208 [in Ukrainian].
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
