FEATURES OF THE FUNCTIONAL STATE OF THE LIVER IN RATS OF DIFFERENT AGE WITH MODELED SECONDHAND TOBACCO SMOKING COMBINED WITH ADMINISTRATION OF MONOSODIUM GLUTAMATE

Authors

  • N. V. Hetsko I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • T. Y. Yaroshenko I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2021.i3.12470

Keywords:

secondhand tobacco smoking, monosodium glutamate, functional state of the liver, biochemical markers

Abstract

Introduction. Every year tobacco smoking kills more that 8 million people all over the world; 1.2 of them are secondhand smokers. Meanwhile, the characteristic feature of modern food technologies is the usage of food supplements which are not always safe for human health.

The aim of the study – to investigate the changes of the liver functional state in blood serum of rats of different age in case of secondhand tobacco smoking combined with administration of monosodium glutamate.

Research Methods. Experiments were conducted on 32 outbred mature white male-rats 180–200 g weight and 32 outbred immature white male-rats 60–80 g weight. Each group of animals was divided into four subgroups: I – control (n=8); ІІ – rats with modeled secondhand tobacco smoking (n=8); ІІІ – rats, which were injected with monosodium glutamate (n=8); IV – rats with modeled secondhand tobacco smoking combined with the monosodium glutamate injection (n=8). In the blood serum of rats, the content of the general bilirubin, the activity of alkaline phosphatase (ALP) and the activity of the alaninaminotransferase (ALT) were determined.

Results and Discussion. In case of secondhand tobacco smoking in the blood serum of mature rats, the content of the general bilirubin significantly decreased by 35.3 %, the ALP activity significantly increased by 38.3 %, the activity of ALT was significantly increased by 2.6 times, which indicated a violation of the functional state of the liver and the development of cytolysis and cholestasis. Secondhand tobacco smoking on the background of the use of monosodium glutamate was accompanied by a decrease in the content of the total bilirubin by 55.3 %; p<0.001 vs control group, which is 31.0 % (p=0.002) below this indicator with an isolated action of tobacco smoke; an increase in ALP activity by 2.1 times; p<0.001 vs control group, which is 53.2 % (p<0.001) exceeded the data with an isolated action of tobacco smoke; an increase in the activity of ALT by 4.5 times; p<0.001 vs control group, which is 68.8 % (p<0.001) exceeded the data with an isolated action of tobacco smoke. In immature rats, the intensity of changes of biochemical markers of the liver functional state exceeds the rates of mature rats.

Conclusions. The results indicate that monosodium glutamate enhances the toxic effect of tobacco smoke on the liver, which is manifested by more pronounced changes in the biochemical markers of its functional state in case of the combined effect of tobacco smoke and monosodium glutamate versus to the isolated tobacco smoke action. In the age aspect, more pronounced changes are observed in immature rats.

References

(2019). World Health Organization. Tobacco; WHO: Geneva, Switerland. Retrieved from: https://www.who. int/news-room/fact-sheets/detail/tobacco

Pinkas, J., Kaleta, D., & Zgliczy ́nski, W.S. (2019). The prevalence of tobacco and E-cigarette use in Poland: A 2019 Nationwide Cross-Sectional Survey. Int. J. Environ. Res. Public Health, 16, 4820.

Yeshchenko, K.N., Zhadan, A.V., & Shustval, N.F. (2013). Serdechno-sosudistaya sistema i kureniye [Cardiovascular system and smoking]. Liky Ukrainy – Medicines of Ukraine, 4 (170), 12-17 [in Russian].

Polka, N.S., Dobryanskaya, O.V., Turos, Ye.I., Dardynskaya, I.V., & Zeygler, D. (2016). Osobennosti rasprastraneniya tabakokureniya sredi shkolnikov Ukrainy [Features of the spread of smoking among school-children of Ukraine]. Zdorovye rebenka – Child’s Health, 6 (74), 27-29 [in Russian].

Ilchenko, S.I., & Fialkovska, A.O. (2018). Personifikovanyy pidkhid do profilaktyky tiuiyunokurinnia u pidlitkiv [Personalized approach to tobacco prevention in adolescents]. Scientific Journal “Science Rise: Medical Science”, 1 (21), 35-38 [in Ukrainian].

Balakiryeva, O.M., Pavlova, D.M., Nhuyen, N.M., Levtsun, O.H., Pyvovarova, N.P., Sakovych, O.T., & Flyarkovska, O.V. (2019). Kurinnia, vzhyvannia alkoholiu ta narkotychnykh rechovyn sered pidlitkiv, yaki navchaiutsia: poshyrennia y tendentsii v Ukraini: Za rezultatamy doslidzhennia 2019 roku v ramkakh mizh­narodnoho proektu “Yevropeyske opytuvannia uchniv shchodo vzhyvannia alkoholiu ta inshykh narkotychnykh rechovyn–ESPAD”. [Smoking, alcohol and drug use among adolescents studying: prevalence and trends in Ukraine: According to the results of a 2019 study within the international project “European survey of students on alcohol and other drug use-ESPAD”.] Kyiv: Polihrafichnyy tsentr Foliant [in Ukrainian].

Pikas, O.B. (2015). Pro stan kurinnia tsyharok u suchasnykh umovakh, yoho vplyv na vynyknennia zakhvoriuvan v orhanizmi liudyny [On the state of cigarette smoking in modern conditions, its impact on the occurrence of diseases in the human body]. Bukovynskyi medychnyi visnyk – Bukovynian Medical Bulletin, 19, 4, 227-230. [in Ukrainian].

Akhavan Rezayat, A., Dadgar Moghadam, M., Ghasemi, Nour. M., Shirazinia, M., Ghodsi, H., Rouhbakhsh Zahmatkesh, M.R., Tavakolizadeh Noghab, I.M., Hoseini, B., Akhavan Rezaya, K. (2018). Association between smoking and non-alcoholic fatty liver disease: A systematic review and meta-analysis. SAGE Open Med, 6, Retrieved from: doi:10.1177/2050312117745223.

Azzalini, L., Ferrer, E., Ramalho, L.N., Moreno, M., Domínguez, M. & Colmenero, J. (2010). Cigarette smoking exacerbates nonalcoholic fatty liver disease in obese rats. Hepatology, 51 (5), 1567-7156.

Zein, C.O., Unalp, A., Colvin, R., Liu, Y.C. & McCullough, A.J. (2011). Nonalcoholic Steatohepatitis Clinical Research Network. Smoking and severity of hepatic fibrosis in nonalcoholic fatty liver disease. J. Hepatol., 54 (4), 753-759.

Dass, B.P., Jaganmohan, P. & Sravanakumar, P. (2013). Changes in hematological and biochemical parameters in smokeless tobacco (ST) Chewers in Costal Belt of Andhra Pradesh, India. European Journal of Biological Sciences, 5 (1), 29-33.

Jang, E.S., Heong, S., Hwang, S., Kim, H., Ahn, S., Lee, J. & Lee, D. (2012). Effects of coffee, smoking and alcohol on liver function tests: a comprehensive cross-sectional study. BMC. Gastroenterology, 12 (1), 145.

Bevzo, V.V. (2016). Doslidzhennia toksykody-namikiv hlutamatu natriiu na tryvalist shchuriv dlia tryvaloho yoho vvedennia [Investigation of the toxi co-dynamics of glutamate sodium in rats under the condition of its long introduction]. Klinichna ta eksperymentalna patolohiia – Clinical and Experimental Pathology, XV, 2 (56), 2, 13-16 [in Ukrainian].

Lizurchik, L.V., & Sheyda, E.V. (2014). Vliyanie tabachnogo dyma na soderzhaniye toksichnykh ele-mentov v organizme krys [Effect of tobacco smoke on the content of toxic elements in the body of rats]. Vestnik OGU – Journal of the Orenburg State University, 6 (167), 71-74 [in Russian].

Santiago, H.A., Zamarioli, A.,Sousa Neto, M.D., & Volpon, J.B. (2017). Exposure to secondhand smoke impairs fracture healing in rats. Clin. Orthop. Relat. Res., 475(3), 894-902.

Falaleeva, T.M., Samonina, H.Ye., Berehova,T.V., Dzyubenko, N.V., & Andreeva, L.A. (2010). Vliyanye gliprolynov na strukturno-funktsyonalnoe sostoyanye slyzystoy obolochky zheludka i massa tela krys v uslo­viyakh dlitelnogo vvedenniya hlutamata natriya. Fizyka zhyvoho – Physics of the Living, 18 (1), 154-159 [in Russian].

Jansen, T., & Daiber, A. (2012). Direct antioxidant properties of bilirubin and biliverdin. Is there a role for biliverdin reductase? Front. Pharmacol., 3, 30.

Alsalhen, K.S., & Abdalsalam, R.D. (2014). Effect of cigarette smoking on liver functions: a comparative study conducted among smokers and non-smokers male in El-beida City, Libya. International Current Pharmaceutical Journal, 3 (7), 291-295.

Tawfik, M.S., & Al-Badr, N. (2012). Adverse effects of monosodium glutamate on liver and kidney functions in adult rats and potential protective effect of vitamins C and E. Food and Nutrition Sciences, 3, 651-659.

Korkin, A.L., Ugorelova, Ye.A., & Gribache­va, A.V. (2016). Diagnosticheskoye znacheniye laboratornykh pokazateley kholestaza i fermentativnogo profilya transaminaz dlya otsenki stepeni bakteriokholii u patsiyentov s zabolevaniyami zhelchnykh putey [Diagnostic value of laboratory parameters of cholestasis and enzymatic profile of transaminases for assessing the degree of bacteriocholia in patients with biliary tract diseases.]. Vestnik SurGU. Meditsina – Bulletin of SURSU. Medicine, 4 (30), 26-29 [in Russian].

Okediran, B.S., Olurotimi, A.E., Rahman, S.A., Michael, O.G. & Olukunle, J.O. (2014). Alterations in the lipid profile and liver enzymes of rats treated with monosodium glutamate. Sokoto Journal of Veterinary Scien­ces, 12 (3), 42-46.

Krynytska, I., Marushchak, M., Svan, O., Akimova, V., Mazur, L. & Habor, H. (2018). The indices of endogenous intoxication in rats with carrageenan solution consumption. Georgian Med. News, 279, 196-200.

Published

2021-12-14

How to Cite

Hetsko, N. V., & Yaroshenko, T. Y. (2021). FEATURES OF THE FUNCTIONAL STATE OF THE LIVER IN RATS OF DIFFERENT AGE WITH MODELED SECONDHAND TOBACCO SMOKING COMBINED WITH ADMINISTRATION OF MONOSODIUM GLUTAMATE . Medical and Clinical Chemistry, (3), 89–96. https://doi.org/10.11603/mcch.2410-681X.2021.i3.12470

Issue

Section

ORIGINAL INVESTIGATIONS