EFFECT OF CRANIOSKELETAL TRAUMA COMPLICATED WITH BLOOD LOSS ON THE ACTIVITY OF CYTOLYSIS AND ENDOGENOUS INTOXICATION IN THE EARLY PERIOD IN RATS WITH DIFFERENT HYPOXIA RESISTANCE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i2.12238Keywords:
cranioskeletal trauma, blood loss, resistance to hypoxia, alanine aminotransferase, middle mass moleculesAbstract
Introduction. Injuries are now considered one of the pressing problems of modern urban society. Its structure is dominated by severe multiple and combined lesions, which are often complicated by multiple organ failure, which becomes the direct cause of death. The work of many authors has shown that the basis of systemic disorders in traumatic illness is the strengthening of cytolysis and endotoxicosis. The formation of cytolysis and endotoxicosis syndrome under conditions of cranioskeletal trauma in animals with different constitutional resistance to hypoxia remains insufficiently studied.
The aim of the study – to determine the dynamics of markers of cytolysis and endotoxicosis in cranioskeletal trauma complicated by blood loss in rats with different resistance to hypoxia in the early period of traumatic illness.
Research Methods. The experiments were performed on 148 nonlinear white male rats weighing 180–200 g, which were on the standard diet of the vivarium. Previously, individual resistance to hypoxia was determined in rats and two groups were identified: high- and low-resistant (HR, LR). In animals, cranioskeletal trauma was simulated and combined with acute blood loss. After 1, 3, and 7 days, the activity of alanine aminotransferase (ALT) and the content of middle mass molecules determined at a wavelength of 280 nm (MМM280) were determined in the serum of the liver of animals resistant to hypoxia.
Results and Discussion. In the pathogenesis of cranioskeletal trauma an important place is occupied by the development of cytolysis syndrome and endotoxicosis, which is manifested by an increase in serum activity of the cytoplasmic enzyme ALT and MМM280 fraction, which reflects the appearance of aromatic amino acids as a result of impaired metabolism. It was found that in emergencies before hypoxia of animals detected violations are greater. Under the conditions of additional modeling of acute blood loss in the range of 20–22 % of the circulating blood volume in the groups of LR and HR of animals, deepening of cytolysis and endotoxicosis processes was noted, especially after 3–7 days of the experiment. Under these conditions, the violation in the serum of ALT activity and the content of the MМM280 fraction in the group of animals was significantly less than in the animals.
Conclusion. Under the conditions of modeling of cranioskeletal trauma in the emergency to hypoxia of animals, the intensification of cytolysis and endotoxicosis is greater than in the group of animals. Disorders in the emergency of animals are exacerbated by additional modeling of acute blood loss.
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