IMMUNE STATUS AND DYNAMICS OF INDICATORS OF THE SYNDROME OF ENDOGENOUS INTOXICATION IN EXPERIMENTAL ACUTE GENERALIZED PERITONITIS AGAINST THE BACKGROUND OF STREPTOSOTOCIN-INDUCED DIABETES MELLITUS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i2.12175Keywords:
acute generalized peritonitis, streptozotocin-induced diabetes mellitus, immune status, syndrome of endogenous intoxicationAbstract
Introduction. The study of pathogenetic features of acute generalized peritonitis against the background of hyperglycemia is an actual issue due to the severity of acute peritonitis, its atypical clinical picture and high mortality in this combined pathology.
The aim of the study – to investigate the immune status and assess the dynamics of endogenous intoxication syndrome in rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes mellitus.
Research Methods. The experimental study was performed on 48 adult white male rats. The main group of animals was divided into three subgroups, in which studied the immune response (Ig G, Ig M, Ig A; CD3+, CD4+, CD8+, CD16+) and indicators of the endogenous intoxication syndrome (MMM254 and MMM280, EII). Diabetes mellitus was simulated by a single intraperitoneal injection of streptozotocin (60 mg/kg). On day 14 of diabetes mellitus, rats were injected with 0.5 ml of 10 % filtered faecal suspension into the abdominal cavity and initiated acute generalized peritonitis. In the control group, rats were injected subcutaneously with 0.9 % NaCl.
Results and Discussion. The tendency to decrease the levels of Ig G and Ig A during all terms of development of the combined pathology is revealed. Excessive accumulation of Ig M and Ig A in the blood on the first day and an increase of level of Ig M on the third day of peritonitis development were observed compared with the control group. Deficiency of T-cell immunity has been established, which is manifested by a decrease in the levels of CD3+, CD4+, CD8+, CD16+ in all subgroups. During the experiment, the progressive increase in endogenous intoxication was accompanied by an increase in level of MMM254 and MMM280 and increase of erythrocyte intoxication index.
Conclusions. In animals with acute generalized peritonitis against the background of streptozotocin-induced diabetes processes of endogenous intoxication progress, which is manifested by an increase of MMM254 and MMM280 in the blood and the value of EII in the dynamics of the combined pathology. Low rates of cellular immune response against the background of high values of peptides with average molecular weight confirm the severity of acute peritonitis under conditions of hyperglycemia.
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