STUDY OF AMINO ACID COMPOSITION OF MEDICINAL HERBAL MIXTURE WITH ANTIDIABETIC ACTIVITY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.i4.11747Keywords:
amino acids, herbal antidiabetic mixture, gas chromato-mass-spectrometry, diabetes mellitusAbstract
Introduction. Diabetes mellitus is one of the priority problems of the WHO, which requires immediate solution, as the epidemiological situation is becoming alarming – the number of patients is growing rapidly each year, leading to increased disability and mortality due to the development of micro- and macroangiopathy. In order to solve this problem, it is necessary to optimize the existing pharmacotherapy, in particular with the help of phytomixtures, which can affect various parts of the pathogenetic mechanism of the disease and its complications due to a wide range of biologically active substances. In this regard, amino acids deserve special attention because they play a key role in many metabolic processes.
The aim of the study – to learn the qualitative composition and quantitative content of amino acids in the herbal antidiabetic mixture and substantiate their effectiveness for the treatment and prevention of diabetes.
Researsch Methods. Determination of the qualitative composition and quantitative content of amino acids was performed by gas chromato-mass-spectrometry.
Results and Discussion. During the study, 8 free amino acids were identified, such as alanine, glycine, valine, threonine, isoleucine, proline, aspartic acid and phenylalanine and 12 related, such as alanine, glycine, valine, leucine, threonine, isoleucine, proline and proline, aline, phenylalanine, lysine and tyrosine. It was found that the studied herbal mixture contains the largest amount of leucine (11.92 mg/g) in bound form, as well as proline in free and bound form – 7.67 mg/g and 11.10 mg/g, respectively.
Conclusions. The results of chromatographic examination indicate a sufficient content of amino acids, in particular essential, which have the ability to stimulate insulin secretion, reduce hyperglycemia and regulate metabolic processes in patients with diabetes. The predominant amino acid component in the herbal antidiabetic mixture is leucine, an essential amino acid with the most pronounced insulin secretolytic activity.
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