THE ROLE OF BIOCHEMICAL DISORDERS IN THE DEVELOPMENT OF ETHANOL FIBROSIS AND CIRRHOSIS OF THE LIVER IN HIGH AND LOW EMOTIONAL RATS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.i4.11739Keywords:
ethanol, liver fibrosis, liver cirrhosis, high- and low-emotional male rats, blood, heartAbstract
Introduction. Excessive alcohol consumption is one of the most common social illness, causing irreversible changes in the body.
The aim of the study – to evaluate the state of the pro- and antioxidant system in the blood and heart of high- and low-emotional male rats (HE, LE) in ethanol fibrosis and cirrhosis of liver.
Research Methods. The experiments were performed on 96 HE and LE outbred rats aged 5.5–6 months. Animals were divided into three groups – control (C), ethanol fibrosis (EF) and ethanol cirrhosis of the liver (EC). Emotionality was determined in the "open field". In blood serum (ser) and heart homogenate (hom) superoxide dismutase (SOD), catalase (Cat) activity, content of diene (DC), triene (TC) conjugates, shiff bases (SB), TBA-active products (TBA) in the blood – peroxidase activity (PA), the content of ceruloplazmin (CP) were determined.
Results and Discussion. In K in HE, in compare with LE, there were smaller DC, TC, TBA, bigger SB. At EF and EC, all indexes in HE and LE increased and were higher in HE. In K in HE OMP370 and OMP430 were bigger in ser, smaller in gom. At EF and EC they increased (except for OMP370 in HE ser). In ser in HE OMP370 were larger. In EC, compared with EF, OMP370 in ser were smaller in HE and LE, and in gom – larger in LE. OMP430 at EC, in comparison with EF, were smaller in ser LE. In K SOD is higher in LE. At EF SOD increased, more in HE, but remained higher in LE. At EC SOD increased more in HE and was higher at them in gom. At EC, in comparison with EF, SOD was bigger. Cat in ser in K was higher in LE. At EF Cat increased and was higher in HE. At EC Cat increased to a greater extent in HE and did not depend on emotionality. At EC, in comparison with EF, Cat was bigger. The CP in ser was higher in HE. At EF and EC it increased, more at LE. There was no difference between CP in EF and EC in HE rats; in LE there were greater values in EC. At EF in HE the content of CP remained higher. PA in K was higher in LE; increased in EF and EC (more). At EC it was higher in HE.
Conclusions. Ethanol fibrosis and cirrhosis of the liver in rats causes damage of the body by an oxidative mechanism. Intensification of LPO is more pronounced in cirrhosis in the serum and heart homogenate of highly emotional animals.
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