PECULIARITIES OF ACTIVATION OF LIPID PEROXIDATION PROCESSES IN THE EARLY PERIOD OF CRANIOSKELETAL INJURY COMPLICATED BY BLOOD LOSS IN RATS WITH DIFFERENT RESISTANCE TO HYPOXIA
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i3.11547Keywords:
cranioskeletal trauma, blood loss, resistance to hypoxia, lipid peroxidationAbstract
Introduction. Injury is one of the current problems. Its structure is dominated by a combined craniocerebral and skeletal trauma, which is accompanied by the highest mortality. One of the markers of traumatic disease is the intensification of lipid peroxidation in tissues and organs, which underlies the pathogenesis of multiorgan failure. Under these conditions, the patterns of lipid peroxidation processes in individuals with different resistance to oxygen deficiency remain virtually unexplored.
The aim of the study – to determine the peculiarities of the activation of lipid peroxidation in the early period of cranioskeletal trauma complicated by blood loss in rats with different resistance to hypoxia.
Research Methods. The experiments were performed on 148 nonlinear white male rats weighing 180–200 g, which were on the standard diet of the vivarium. Previously, individual resistance to hypoxia was determined in rats and two groups were identified: high- and low-resistance. In animals, cranioskeletal trauma was simulated and combined with acute blood loss. After 1, 3 and 7 days in the liver of animals resistant to hypoxia, the content of reagents for thiobarbituric acid was determined – a screening marker of lipid peroxidation intensification.
Results and Discussion. In response to cranioskeletal trauma in experimental animals there is an activation of lipid peroxidation of cell membranes of hepatocytes. Under conditions of additional blood loss, there is a layering of the influence of pathogenic mechanisms of trauma and hypoxemia, in which there is an increase in the formation of reagents in the liver to thiobarbituric acid. In highly resistant animals to hypoxia, the degree of accumulation of reagents for thiobarbituric acid in the liver was significantly lower than in low-resistant animals at all times. The obtained results indicate that in highly resistant animals, despite the higher intensity of lipoperoxidation in the initial state, the reaction to trauma is accompanied by less activation of lipid peroxidation processes than in low-resistant animals. This suggests a lower probability of developing multiorgan failure in highly resistant animals, which is of great practical importance and requires further study.
Conclusion. In response to cranioskeletal trauma and its combination with acute blood loss in the acute period and the period of early manifestations of traumatic disease compared with control, there is an increase in the intensity of lipid peroxidation in the liver of highly and low hypoxia-resistant animals.
References
MacKay, E.J., Stubna, M.D., Holena, D.N., Reilly, P.M., Seamon, M.J., Smith, B.P., … Cannon, J.W. (2017). Abnormal calcium levels during trauma resuscitation are associated with increased mortality, increased blood product use, and greater hospital resource consumption: a pilot investigation. Anesthesia and Analgesia, 125, 895-901. DOI: https://doi.org/10.1213/ANE.0000000000002312
Huryev, S.O., Fil, A.Yu., & Tanasiienko, O.M. (2015). Analiz bezposeredikh prychyn smerti u postrazhdalykh iz politravmoiu ta poshkodzhenniam skeleta [Analysis of direct causes of death in victims with polytrauma and skeletal damage]. Travma – Trauma, 16, 4, 7-10 [in Ukrainian].
Inozemtsev, Ye.O., Grigoryev, Ye.G., & Apartsin, K.A. (2017). Aktualnyye voprosy khirurgii sochetannykh povrezhdeniy [Current issue of surgery of associated injuries]. Politravma – Polytrauma, 1, 6-11 [in Russian].
Kardash, A.M., Cherniy, V.Í., Gorodnik, G.A., & Botev, V.S. (2014). Monitoring i lecheniye tyazheloy cherepno-mozgovoy travmy [Monitoring and treatment of severe traumatic brain injury]. Ukrainskyi neirokhirurhichnyi zhurnal – Ukrainian Neurosurgical Journal, 2, 8-19 [in Russian].
Fil, A.Yu. (2014). Khirurhichna kontseptsiia likuvannia postrazhdalykh iz politravmoiu u hostromu periodi [Surgical concept of treatment of victims with polytrauma in the acute period]. Travma – Trauma, 15, 5, 20-23 [in Russian].
Borys, R.M., Hozhenko, A.I., & Hudyma, A.A. (2013). Dynamika pokaznykiv peroksydnoho okysnennia lipidiv u period rannikh i piznikh proiaviv travmatychnoi khvoroby za umov eksperymentalnoi kranioskeletnoi travmy ta yii korektsii klitynnoyu terapiieiu [Dynamics of lipid peroxidation indicators in the period of early and late manifestations of traumatic disease under the conditions of experimental cranioskeletal trauma and its correction by cell therapy]. Klinichna ta eksperymentalna patolohiia – Clinical and Experimental Pathology, KHII, 2 (44), 31-34 [in Ukrainian].
Servatovych, A.M. (2015). Dynamika porushen protsesiv lipidnoi peroksydatsii v period piznikh proiaviv travmatychnoi khvoroby pislia kranioskeletnoi travmy ta efektyvnist korektsii roslynnym ekstraktom [Dynamics of disturbance processes of lipid peroxidation during the late ma nifestations of traumatic disease after cranioskeletal injury and effectiveness of its correction by phytomixtur]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 4, 106-109 [in Ukrainian].
Voloshenyuk, A.N., Zavada, N.V., Stebunov, S.S., Khilimon, D.A., Shnayder, A.G., & Filinov, S.V. (2012). Analiz gospitalnoy letalnosti pri sochetannoy travme [Analysis of hospital mortality in concomitant injury]. Ekstrennaya meditsina – Emergency Medicine, 2, 67-74 [in Russian].
Konovchuk, V.M. (2009). Hemorahichnyi hipovolemichnyi shok ta rozvytok poliorhannoho ushkodzhennia [Hemorrhagic hypovolemic shock and development of multiorgan damage]. Bukovynskyi medychnyi visnyk – Bukovynian Medical Bulletin, 13, 1, 12-17 [in Russian].
Horban, I.I., Hudyma, A.A., Maksymiv, R.V., & Antonyshyn, I.V. (2020). Influence of two-hour tourniquets ischemia of limb and acute blood loss on systemic disorders of the body in the reperfusion period (experimental study). Wiadomości Lekarskie, LXXIII, 7, 1330-1333. DOI: https://doi.org/10.36740/WLek202007104
Saturska, H.S., & Bondarenko, Yu.I. (2014). Osoblyvosti metabolizmu spoluchnoi tkanyny pry eksperymentalnomu dyfuznomu ishemichno-nekrotychnomu kardiosklerozi u shchuriv iz riznoiu stiikistiu do hipoksii [Peculiarities of connective tissue metabolism in experimental diffuse ischemic-necrotic cardiosclerosis in rats with different resistance to hypoxia]. Visnyk VNMU – Bulletin of VNMU, 18, 2, 425-429 [in Ukrainian].
Andreyeva, L.I., Kozhemyakin, L.A., & Kishkun, A.A. (1988). Modifikatsiya metoda opredeleniya perekisey lipidov v teste s tiobarbiturovoy kislotoy [Modification of the method for determining lipid peroxides in the test with thiobarbituric acid]. Lab. delo. – Lab. Matter, 11, 41-43 [in Russian].
Kseyko, D.A., & Gening, T.P. (2012). Protsessy perekisnogo okisleniya lipidov i zashchitnaya rol antioksidantnoy sistemy v pecheni i eritrotsitakh v usloviyakh ostroy krovopoteri [The processes of lipid peroxidation and the protective role of the antioxidant system in the liver and erythrocytes in conditions of acute blood loss]. Fund. Issled. – Fund. Res., 9-2, 304-307 [in Russian].
Bayburina, G.A., Nurgaleyeva, Ye.A., Bashkatov, S.A., & Shibkova, D.Z. (2015). Vzaimozavisimost pokazateley svobodnoradikalnogo okisleniya v pecheni i krovi u krys s raznoy ustoychivostyu k gipoksii posle perenesonnoy anoksii [The interdependence of the indices of free radical oxidation in the liver and blood in rats with different resistance to hypoxia after anoxia]. Kazanskiy meditsinskiy zhurnal – Kazan Medical Journal, 96, 5, 798-802 [in Russian].
Posokhova, K.A., & Berezhna, I.Yu. (2002). Osoblyvosti perebihu hemichnoi hipoksii i efektyvnist preparativ antyoksydantnoi i antyhipoksantnoi dii u tvaryn z riznoiu rezystentnistiu do hipoksii [Peculiarities of hemic hypoxia and efficiency of antioxidant and antihypoxant drugs in animals with different resistance to hypoxia]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 1, 105-108 [in Ukrainian].
Grek, O.R., Sharapov, V.I., Tikhonova, Ye.V., Mishenina, S.V., Shishkanova, A.V., Sharapov, I.V., & Ravilova, Yu.R. (2011). Vliyaniye ostroy gipoksii na antiokislitelnuyu aktivnost tkani pecheni u krys s raznoy ustoychivost’yu k gipoksii [The effect of acute hypoxia on the antioxidant activity of liver tissue in rats with different resistance to hypoxia]. Vestn. nov. med. tekhnol. – Bull. New Med. Tech., 18, 4, 62-64 [in Russian].
