DISTURBANCE OF THE CONTENT OF RESTORATED GLUTATION IN THE LUNGS OF RATS ON THE BACKGROUND OF ACUTE BLOOD LOSS COMPLICATED BY ISCHEMIA-REPERFUSION OF THE LIMBS AND THEIR CORRECTION WITH CARBACETAM
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i3.11537Keywords:
acute blood loss, ischemia-reperfusion of the limb, lungs, reduced glutathione, carbacetamAbstract
Introduction. The lungs are organs that are particularly sensitive to systemic pathogens caused by acute blood loss and ischemic-reperfusion syndrome. They are based on the activation of lipoperoxidation processes with depletion of antioxidant protection. However, the dynamics of the content of reduced glutathione in the lungs under conditions of acute blood loss, ischemia-reperfusion of the limb and their combination is insufficiently studied, there is no data on the corrective effect of carbacetam.
The aim of the study – to determine the features of disorders of the content of reduced glutathione in the lungs of rats in the dynamics of acute blood loss complicated by ischemia-reperfusion of the limb, and the effectiveness of their correction with carbacetam.
Research Methods. The study was performed on 216 white nonlinear male rats weighing 200–220 g. All experiments were performed under thiopental-sodium anesthesia. In animals, limb ischemia-reperfusion and acute blood loss were simulated and these lesions were combined. In a separate group, the detected disorders were corrected with carbacetam. After 1 and 2 hours, as well as after 1, 7 and 14 days in the lungs of experimental animals was determined the content of reduced glutathione.
Results and Discussion. Under conditions of ischemia-reperfusion of the limb, the content of reduced glutathione in the lungs after 1 h of the experiment compared with the control increases significantly, which is obviously compensatory. In the future, the rate is statistically likely to decrease, but up to 14 hours again increases and reaches the level of control. In the conditions of acute blood loss, the content of reduced glutathione in all terms of observation in comparison with the control is significantly reduced and becomes smaller in comparison with the group in which only ischemia-reperfusion of the limb was modeled. After the complication of acute blood loss by ischemia-reperfusion of the limb, the disorders of the glutathione antiperoxidase system became even greater, especially after 7 and 14 days of the experiment. The use of carbacetam in conditions of ischemia-reperfusion of the limb, acute blood loss and their combination is accompanied by a pronounced positive effect, which was to reduce the cost of reduced glutathione in the neutralization of prooxidants and endotoxins in the lungs.
Conclusions. Simulation of acute blood loss and its complication by limb ischemia-reperfusion compared with control is accompanied by a decrease in the content of reduced glutathione in the lungs from 1 h to 14 days. Additional ischemia-reperfusion of the limb after 7 and 14 days causes a statistically greater decrease in the studied indicator. The use of carabcetam in these conditions compared with animals without correction in the lungs after 7 and 14 days is accompanied by less depletion of reduced glutathione.
References
Marushchak, M.I., Savchuk, S.O., Hevko, U.P., & Oliynyk, O.V. (2017). Osoblyvosti antoksydantnoi systemy zakhystu u dynamitsi rozvytku hostroho respiratornoho dystres-syndromu ta pry riznykh metodakh korektsii u shchuriv [Peculiarities of antioxidant protection system in the dynamics of acute respiratory distress syndrome development and at different methods of its correction in rats]. Scientific Journal “ScienceRise: Medical Science”, 6 (14), 18-23 [in Ukrainian].
Rubenfeld, G.D., Caldwell, E., Peabody, E., Weaver, J., Martin, D.P., Neff, M., …, & Hudson, L.D. Incidence and outcomes of acute lung injury (2005). New Engl. J. Med., 353, 5, 1685-1693.
Shlaifer, A., Yitzhak, A., Baruch, E.N., Shina, A., Satanovsky, A., Shovali, A., …, & Glassberg, E. (2017). Point of injury tourniquet application during Operation Protective Edge-What do we learn? J. Trauma Acute Care Surg., 83, 2, 278-283. DOI: https://doi.org/10.1097/TA.0000000000001403
Hudyma, A.A., Kashchak, T.V., & Shepitko, K.V. (2019). Antyoksydantno-prooksydantnyi ta tsytokinovyi balans u piznii period kombinovanoi travmy v eksperymenti [Antioxidant-prooxidant and cytokine balance in the late period of combined trauma in the experiment]. Svit medytsyny ta biolohii – World of Medicine and Biology, 1 (67), 42-47 [in Ukrainian].
Shatskyy, V. (2018). Vplyv arterialnoho dzhhuta i reperfuzii kintsivky na dynamiku aktyvnosti superoksyddysmutazy i katalazy u nyrtsi [Influence of arterial tourniquet and limb reperfusion on the dynamics of superoxide dismutase and catalase activity in the kidney]. Proceedings of the Сongress "XKHII Mizhnar. med. konhr. studentiv i molodykh vchenykh – XXII International Med. Congr. of Students and Young Scientists. (pp. 277–278). Ternopil: Ukrmedknyha [in Ukrainian].
Horban, I.I., Hudyma, A.A., Maksymiv, R.V., & Dzhavadova, N.A. (2020). Peculiarities of impairments of absorptive-excretory and glycogen synthesis functions of the liver in the presence of acute blood loss complicated by limb ischemia-reperfusion and their correction by carbacetam. Journal of Education, Health and Sport, 10 (5), 301-314. DOI: https://doi.org/10.12775/JEHS.2020.10.05.032
Maksymiv, R.V., Hudyma, A.A., & Sydorenko, V.M. (2017). Dynamika balansu antyoksydantno-prooksydantnykh mekhanizmiv u vnutrishnikh orhanakh pid vplyvom arterialnoho dzhhuta i reperfuzii kintsivky [Dynamic of balance of antioxidant-prooxidant mechanism in internal organs Under the influence of blood harnesses and limb reperfusion]. Shpytalna khirurhiia. Zhurnal imeni L.Ya. Kovalchuka – Hospital Surgery. Journal named by L.Ya. Kovalchuk, 1, 37-44 [in Ukrainian].
Volotovska, N.V., Nhokwara, T.C., & Zhulkevych, I.V. (2019). Changes in the glutathione systems activity of internal organs in the first hours of experimental limb ischemia-reperfusion syndrome, combined with blood loss and mechanical injury. Zdobutky klinich. i eksperym. medytsyny – Achievements of Clinical and Experiment. Medicine, 1, 23-27.
Popova, T.N., Pashkov, A.N., & Semenikhina, A.V. (2008). Svobodnoradikalnyye protsessy v biosistemakh [Free radical processes in biosystems]. Voronezh [in Russian].
Shatskyi, V.V., & Hudyma, A.A. (2019). Significance of lipid peroxidation processes in renal excretory dysfunction in cases of acute blood loss complicated by limb ischemia-reperfusion and its correction. Journal of Education, Health and Sport, 9, 11, 293-306. DOI: https://doi.org/10.12775/JEHS.2019.09.11.027
Ellman, G.L. (1959). Tissue sulfhydryl groups. Arch. Biochem. Biophys, 82, 1, 70-77. DOI: https://doi.org/10.1016/0003-9861(59)90090-6
Zyblev, S.L., & Dundarov, Z.A. (2013). Sostoyaniye metabolizma pri eksperimentalnoy ostroy massivnoy krovopotere v zavisimosti ot provodimoy terapii [The state of metabolism in experimental acute massive blood loss, depending on the conducted therapy]. Novosti khirurgii – Surgery News, 21, 5, 3-10 [in Russian]. DOI: https://doi.org/10.18484/2305-0047.2013.5.3
