PECULIARITIES OF CHANGES IN FATTY ACID COMPOSITION OF PHOSPHOLIPIDS AND LIVER STRUCTURES AT RESECTION OF DIFFERENT VOLUMES OF ITS PARENCHYMA
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i3.11530Keywords:
liver resection, fatty acid composition of phospholipids, liver structureAbstract
Introduction. Postresection portal hypertension leads to structural rearrangement of vessels and organs of the portal hepatic vein, as well as functional and morphological changes of the liver, which are insufficiently studied.
The aim of the study – to learn the features of changes in the fatty acid composition of phospholipids and liver structures at resection of different volumes of its parenchyma.
Research Methods. Biochemical and morphological methods were used to study the fatty acid composition of liver phospholipids and its structure in 45 laboratory adult white male rats, which were divided into 3 groups. Group 1 had 15 intact animals (control), 2 – 15 rats after resection of the left lateral lobe – 31.5 % of the liver parenchyma, 3 – 15 animals after removal of the right and left lateral lobes of the liver (58.1 %). Euthanasia of rats was performed by bloodletting under conditions of thiopental anesthesia 1 month after the start of the experiment. Вiochemically determined the fatty acid composition of some phospholipids of the liver. Histological micronutrients were made from the liver. Morphometry of hepatocytes and branches of the hepatic portal vein was performed. Quantitative values were processed statistically.
Results and Discussion. It was found that the removal of the left and right lobes of the liver in white rats leads to postresection portal hypertension, pronounced changes in the fatty acid composition of phospholipids and liver structures. The most pronounced changes were found in the branches of the portal hepatic vein. Correlations between the fatty acid composition of phospholipids and liver structures have been revealed.
Conclusions. Removal of the left and right lobes of liver in white rats leads to postresection portal hypertension, marked changes in fatty acid composition of phospholipids and liver structures, which are morphometrically characterized by a significant increase in hepatocytes, their nuclei, nucleo-cytoplasmic and stromal parenchymal relation relative volume damage parenchymal cells, endotheliocytes, infiltrative and sclerotic processes. Structural changes in the branches of the hepatic portal vein dominated.
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