DETOXIFYING AND ANTIOXIDANT EFFECT OF AMINOACIDS IN RATS INTOXICATED WITH SODIUM NITRITE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i3.11475Keywords:
aminoacids, antioxidantsystem, blood, enzymes, sodium nitrite, erythrocytesAbstract
Introduction. The growing use of nitrogen fertilizers is a major source of nitrates and nitrites in the environment. Nitrites in animals change the valence of iron in hemoglobin, converting it to methemoglobin, which is unable to combine with oxygen in the lungs and be converted to oxyhemoglobin. Accordingly, the main function of hemoglobin is lost – to bind oxygen and deliver it to the tissues. As a result, hypoxia develops. Nitrates and nitrites are antispasmodic poisons. The destructive effect of nitrites and nitrates on the body is due to the initiation of free radical processes and lipid peroxidation, which leads to disruption of cell membranes, reduced activity of the immune system.
The aim of the study – to learn the effect of amino acids on individual indicators of the antioxidant system of rats under the action of sodium nitrite.
Research Methods. The studies were performed on laboratory white male rats injected intraperitoneally with sodium nitrite and additionally glutamic acid and cysteine. The animals were fed standard feed for laboratory rats. All rats had a free access to a standard rodent diet and water ad libitum. They were housed in cages under standard laboratory conditions with a 12-h light/12-h dark cycle. A number of antioxidant protection enzymes were determined in erythrocytes.
Results and Discussion. It was found that the use of sodium nitrite led to changes in indicators of the antioxidant defense system. The result ssuggest that the level of GSH and activity of glutathione peroxidase and catalase decreased in the red cells of animals. Animals of the experimental groups that received glutamic acid and cysteine after the sodium nitrite underwent less noticeable changes compared to the control.
Conclusions. The results suggest that the level of GSH and activity of glutathione peroxidase and catalase decreased in the red cells of animals. Less noticeable changes were found in animals that additionally received the studied amino acids.
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