DEVELOPMENT OF A SPECTROPHOTOMETRIC METHOD OF DETERMINATION OF SUM OF FLAVONOIDS IN THYME CREEPING EXTRACTS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i2.11366Keywords:
thyme creeping, flavonoids, extract, quantification, spectrophotometry, acceptability criteriaAbstract
Introduction. Nowadays, there is a growing scientific interest in the study of flavonoids in medicinal plant raw material, which is associated with their high biological activity and diverse pharmacological action [1]. An accessible and promising source of biologically active substances is the well-known thyme creeping (TC). Analyzing the literary data, medicines based on it are widely used in folk and practical medicine, which makes thyme creeping relevant for the development of new domestic plant extracts. The development of a new mucolytic means on the basis of thick extract of thyme creeping and essential oil of thyme ordinary envisaged the receipt of liquid and thick thyme creeping extract. Last one got on technology, which resulted in extracts with satisfactory pharmaco-technological properties and maximum and stable content of BAS of the studied raw material. In previous researches the analysis of thyme creeping was conducted, and, in order to standardize this raw material as an indicator of quality and among others, the composition and content of flavonoids and hydroxycitric acids was chosen. The complex combinations of BASs available in extracts require the use of modern methods of analysis for their standardization and, in the future, ready-to-use medical products extracts. After realization of quality analysis of flavonoids and hydroxybutyric acids in these extracts, it is also necessary to define their quantitative content, that will allow to offer a quantitative index therefore appropriate is realization of authentication and quantitative determination of investigated BAS as basic indexes of quality of the quality and acceptability criteria in carrying out the standardization of the received extracts of TC.
The aim of the study – to work out methodology of quantative determination of flavonoids in liquid and thick (TC) extracts and to select appropriate acceptability criteria.
Research Мethods. Cary-50 spectrophotometer, (FSZ) apigenin (Fluka), liquid and thick thyme creeping extract, ethyl alcohol 60% (v / v), 3 % aluminum chloride solution R were used in the study.
Results and Discussion. The content of flavonoids in the obtained TC extracts was determined by differential spectrophotometry by the reaction of formation of a photometer compound with aluminum chloride. The quantitative content of flavonoids in the investigated TC extracts varies within certain limits and is determined by their content in the feedstock and reproducibility of the extract technology. For the standardization of the got thyme creeping extracts it is possible to offer quantitative quality criteria of flavonoids content not less than 0.03 %, thick extract – not less than 2.5 % in terms of apigenin.
Conclusions. A method for quantifying the amount of flavonoids in thyme creeping extracts was worked out. As a result, their quantitative content is determined and acceptability criteria were proposed.
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