YOUDEN’S TEST OF THE CHROMATOGRAPHIC DETERMINATION OF ATENOLOL IN PHARMACEUTICALS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i2.11361Keywords:
atenolol, high-performance liquid chromatography, robustness, quantitative analysis, Youden’s testAbstract
Introduction. Robustness tests were originally introduced to avoid problems in interlaboratory studies and to identify the potentially responsible factors. Performing a robustness test late in the validation procedure involves the risk that when a method is found not to be robust, it should be redeveloped and optimised. At this stage much effort and money have already been spent in the optimisation and validation, and therefore one wants to avoid this.
The aim of the study – to evaluate the rubustness of HPLC determination of atenolol in tablets using Youden’s test.
Research Methods. An efficient method to assess the robustness of analytical methods is by Youden’s test, by means of an experiment design which involves seven analytical parameters combined in eight tests. In the recent studies, we assessed the robustness of a chromatographic method to quantify enalapril in tablets using Youden’s test.
Results and Discussion. By using the Youden’s test criteria, HPLC method showed to be greatly robust concerning atenolol content, at the introduction of variation in seven analytic parameters. The lowest variation in atenolol content was 0.96 %, when was used column Zorbax C8 (4.6 mm i.d. ×150 mm, 5 μm). For the first time, a holistic approach involving simultaneous innovations in particle technology and instrument design was endeavored to meet and tackle the issues of the analytical laboratory. This was done in order to make analytical scientists more successful and businesses more profitable and productive.
Conclusion. Youden’s test proved to be an efficient and helpful tool for the robustness evaluation for assay of atenolol by HPLC. Youden’s test can be applied successfully for the robustness evaluation in validation process of analytical methods.
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