ENDOGENIC INTOXICATION IN RATS WITH EXPERIMENTAL CARCINOGENESIS AFTER APPLICATION OF CYTOSTATICS ON THE BACKGROUND OF SORPTION THERAPY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i2.11356Keywords:
oxidative stress, antioxidant system, dimethylhydrazine, enterosorbent AUT, cytostatic VincristineAbstract
Introduction. Every year the number of cancer patients with colorectal cancer is growing. The severity of the condition of patients is often due to hyperfunction of reactive oxygen species with subsequent violation of the body's antioxidant defenses. Therefore, it is important to study the effectiveness of cytostatics on the background of enterosorption therapy in conditions of endogenous intoxication.
The aim of the study – to evaluate the effectiveness of the use of the cytostatic Vincristine on the background of enterosorption therapy under conditions of oxidative stress in the body of rats affected by 1,2-dimethylhydrazine
Research Methods. The experiments were performed on white male rats simulated for colon cancer by weekly subcutaneous administration of 1,2-dimethylhydrazine at a dose of 7.2 mg/kg body weight for 30 weeks. Enterosorbent AUT was administered intragastrically daily for 21 days after modeling carcinogenesis at a dose of 1 ml of suspension (corresponding to 0.2 g of net weight of the drug) per 100 g of body weight of the animal. The cytostatic Vincristine was administered intragastrically daily to animals with induced carcinogenesis after 21 days of detoxification therapy for 14 days at a dose of 0.23 mg/kg body weight. The development of oxidative stress was studied by the activity of oxidative modification of proteins (OMB), the concentration of lipid peroxidation products (LPO) by the activity of superoxide dismutase (SOD), catalase.
Results and Discussion. The results of the study indicate that a 30-week administration of the xenobiotic 1,2-DMG promotes the development of oxidative stress. In experimental animals, the peroxidant and antioxidant balance is disturbed, which is accompanied by a decrease in the antioxidant system. The use of enterosorbent AUT contributed to the normalization of these parameters, and the cytostatic therapy had a negligible effect on the course of oxidative processes in the body of experimental animals.
Conclusion. The obtained results indicate the absence of a pronounced side effect of the cytostatic Vincristine and confirm the positive dynamics of the use of detoxification therapy with AUR sorbent during the progressive development of oxidative stress under the conditions of simulated carcinogenesis.
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